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RPS17 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8119148

Background: Human ribosomal protein S17 (RPS17) is mutated in Diamond-Blackfan Anemia (DBA), a bone marrow disorder that fails to produce sufficient red blood cells leading to anemia. Mutations in ribosomal proteins RPS19, RPS24, and RPS17 have been reported… More details

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Product Overview

Online Datasheet in PDF
Product Name
RPS17, Polyclonal Antibody
Full Product Name
Anti-RPS17 Antibody, Rabbit Polyclonal
Product Gene Name
anti-RPS17 antibody
Product Synonym Names
RPS17 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-DBA4 Antibody; Anti-RPS17L Antibody; Anti-RPS17L1 Antibody; Anti-RPS17L2 Antibody; Anti-S17 Antibody; ribosomal protein S17
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. PBS, pH7.0 with 0.03% Proclin300
Purity/Purification
Protein A & Antigen Affinity
Specificity
Human RPS17
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Immunogen
E Coli-derived Human RPS17 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human RPS17 fragment, and purified by antigen affinity chromatography.
Applications Tested/Suitable for anti-RPS17 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes for anti-RPS17 antibody
WB: 1:500-1:2000
IHC-P: 1:500-1:2000
ICC/IF: 1:50-1:200
IP: 1-5uL/mg of lysate

Immumohistochemistry (IHC) of anti-RPS17 antibody

Immunochemical staining of human RPS17 in human brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Rabbit Anti-RPS17 Polyclonal Antibody – Cat# MBS8119148

Immunofluorescence (IF) of anti-RPS17 antibody

Immunofluorescence staining of RPS17 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti- RPS17 polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor-488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Rabbit Anti-RPS17 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS8119148

Immunoprecipitation (IP) of anti-RPS17 antibody

RPS17 was immunoprecipitated using:
Lane A:0.5 mg HeLa Whole Cell Lysate
Lane B:0.5 mg NCI-H292 Whole Cell Lysate
4 uL anti- RPS17 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.
Primary antibody:
Anti- RPS17 rabbit polyclonal antibody,at 1:100 dilution
Secondary antibody:
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15kDa
Observed band size :15 kDa
Rabbit Anti-RPS17 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS8119148

Product References and Citations for anti-RPS17 antibody

Kenney SP, et al. (2015) Identification and fine mapping of nuclear and nucleolar localization signals within the human ribosomal protein s17. PLoS One 10 (4): e0124396.Chen IT, et al. (1988) The transcriptionally active human ribosomal protein s17 gene. Gene 70 (1): 107-116.Ba¤uerle A, et al. (1995) Nucleotide sequence of the gene for ribosomal protein s17 from dictyostelium discoideum. Biochim Biophys Acta 1260 (2): 223-226.

Other Notes

Small volumes of anti-RPS17 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RPS17 purchase

MBS8119148 is an antibody targeting RPS17. Also known as Anti-RPS17 Antibody, Rabbit Polyclonal. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RPS17. It may not necessarily be applicable to this product.

Protein Family
40S ribosomal protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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