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RRP8 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4504916

MBS4504916 is an antibody targeting RRP8. Also known as Rabbit anti-RRP8 Antibody. Reactive in Human. Host: Rabbit. More details

$235 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RRP8, Polyclonal Antibody
Full Product Name
Rabbit anti-RRP8 Antibody
Product Gene Name
anti-RRP8 antibody
Product Synonym Names
KIAA0409; NML; Ribosomal RNA-processing protein 8; Cerebral protein 1; Nucleomethylin
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL (lot specific)
Preparation and Storage
Store at -20 degree C. Avoid repeated freeze/thaw cycles.
Molecular Weight
50,715 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (6621144..6624880, complement). Location: 11p15.4
3D Structure
ModBase 3D Structure for O43159
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RRP8. The exact sequence is proprietary.

Product Categories/Family for anti-RRP8 antibody

Primary antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-RRP8 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-RRP8 antibody
WB: 1:500-1:1000
IHC: 1:100-1:200

Western Blot (WB) of anti-RRP8 antibody

Western blot analysis of RRP8 expression in Hela (A), MCF7 (B), Hela (C) whole cell lysates.
Rabbit Anti-RRP8 Polyclonal Antibody – Cat# MBS4504916

Immunohistochemistry (IHC) of anti-RRP8 antibody

Immunohistochemical analysis of RRP8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-RRP8 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4504916

Other Notes

Small volumes of anti-RRP8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RRP8. It may not necessarily be applicable to this product.

NCBI GI #
12758125
NCBI GeneID
23378
NCBI Accession #
NP_056139.1
UniProt Primary Accession #
O43159
UniProt Secondary Accession #
Q7KZ78; Q9BVM6
UniProt Related Accession #
O43159
GenBank Nucleotide #
NM_015324.3
NCBI Official Full Name
ribosomal RNA-processing protein 8
NCBI Official Synonym Full Names
ribosomal RNA processing 8, methyltransferase, homolog (yeast)
NCBI Official Symbol
RRP8
NCBI Official Synonym Symbols
KIAA0409
UniProt Protein Name
Ribosomal RNA-processing protein 8
UniProt Synonym Protein Names
Cerebral protein 1; Nucleomethylin
Protein Family
25S rRNA
UniProt Gene Name
RRP8
UniProt Synonym Gene Names
KIAA0409; NML
UniProt Entry Name
RRP8_HUMAN
NCBI Protein Information
ribosomal RNA-processing protein 8; nucleomethylin; cerebral protein 1; RRP8 methyltransferase homolog

UniProt Comments for RRP8

RRP8: Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys- 9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown. Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8. Belongs to the methyltransferase superfamily. RRP8 family.

Protein type: EC 2.1.1.-; RNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: nucleoplasm; chromatin silencing complex; cytoplasm; plasma membrane; nucleolus; nucleus

Molecular Function: protein binding; S-adenosylmethionine-dependent methyltransferase activity; methylated histone residue binding

Biological Process: methylation; chromatin silencing at rDNA; cellular response to glucose starvation; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; gene expression; chromatin modification; regulation of gene expression, epigenetic; regulation of transcription by glucose; rRNA processing
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Research Articles on RRP8

1. These observations suggest that NML may regulate consumption of hepatic triglyceride in liver regeneration after partial hepatectomy due to storage of excess ATP.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-RRP8 antibody

 Products by Pathway  Pathway Diagram
 Epigenetic Regulation Of Gene Expression Pathway antibodies  Epigenetic Regulation Of Gene Expression Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Negative Epigenetic Regulation Of RRNA Expression Pathway antibodies  Negative Epigenetic Regulation Of RRNA Expression Pathway Diagram
 SIRT1 Negatively Regulates RRNA Expression Pathway antibodies  SIRT1 Negatively Regulates RRNA Expression Pathway Diagram
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