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RSK1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6498257

MBS6498257 is an antibody targeting RSK1. Also known as RSK1 (Ser732) (Ribosomal S6 Kinase 1, RSK-1, 90kD Ribosomal Protein S6 Kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP Kinase 1a,… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
RSK1, Polyclonal Antibody
Full Product Name
RSK1 (Ser732) (Ribosomal S6 Kinase 1, RSK-1, 90kD Ribosomal Protein S6 Kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP Kinase 1a, MAPKAPK1A, MAPKAPK-1a, Ribosomal Protein S6 Kinase alp
Product Gene Name
anti-RSK1 antibody
Product Synonym Names
Ribosomal Protein S6 Kinase 90kDa Polypeptide 1; EC=2.7.11.1; HU-1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Immunoaffinity chromatography.
Specificity
Recognizes human RPS6KA1.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
82.723
OMIM
601684
3D Structure
ModBase 3D Structure for Q15418
Immunogen
Synthetic peptide corresponding to amino acid residues surrounding S732 of human RPS6KA1 (KLH).
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-RSK1 antibody

Antibodies; Protein Kinases; Ribosomal
Applications Tested/Suitable for anti-RSK1 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-RSK1 antibody
IHC: 1:10-1:50
ELISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-RSK1 antibody

Western blot analysis of RPS6KA1 (arrow) using rabbit polyclonal RPS6KA1 Antibody (S732). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RPS6KA1 gene (Lane 2) (Origene Technologies).
Rabbit Anti-RSK1 Polyclonal Antibody – Cat# MBS6498257

Immunohistochemistry (IHC) of anti-RSK1 antibody

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with RPS6KA1 Antibody (S732), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-RSK1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6498257

Product References and Citations for anti-RSK1 antibody

1. Cavet, M.E., et al., J. Biol. Chem. 278(20):18376-18383 (2003). 2. Versteeg, H.H., et al., J. Biol. Chem. 277(30):27065-27072 (2002). 3. Fingar, D.C., et al., Genes Dev. 16(12):1472-1487 (2002). 4. Lu, H., et al., J. Pharmacol. Exp. Ther. 300(3):818-823 (2002). 5. Saitoh, M., et al., J. Biol. Chem. 277(22):20104-20112 (2002).

Other Notes

Small volumes of anti-RSK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RSK1. It may not necessarily be applicable to this product.

NCBI GI #
20149547
NCBI GeneID
6195
NCBI Accession #
NP_002944
UniProt Primary Accession #
Q15418
UniProt Related Accession #
Q15418
GenBank Nucleotide #
NP_002944.2
NCBI Official Full Name
ribosomal protein S6 kinase alpha-1 isoform a
NCBI Official Synonym Full Names
ribosomal protein S6 kinase A1
NCBI Official Symbol
RPS6KA1
NCBI Official Synonym Symbols
RSK; HU-1; RSK1; p90Rsk; MAPKAPK1; MAPKAPK1A
UniProt Protein Name
Ribosomal protein S6 kinase alpha-1
UniProt Synonym Protein Names
90 kDa ribosomal protein S6 kinase 1; p90-RSK 1; p90RSK1; p90S6K; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; Ribosomal S6 kinase 1
UniProt Gene Name
RPS6KA1
UniProt Synonym Gene Names
MAPKAPK1A; RSK1; S6K-alpha-1; p90-RSK 1; p90RSK1; p90S6K; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; RSK-1
UniProt Entry Name
KS6A1_HUMAN
NCBI Protein Information
ribosomal protein S6 kinase alpha-1

NCBI Summary for RSK1

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
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UniProt Comments for RSK1

p90RSK: an AGC kinase of the RSK family. Phosphorylated and activated by Erk1 and -2 in response to many growth factors, polypeptide hormones and neurotransmitters. Several phosphorylation sites are important for its activation. Possesses two kinase domains connected by a regulator linker region. Phosphorylates a wide range of substrates including ribosomal protein S6. Prominently expressed in brain structures essential for cognitive function and learning.

Protein type: EC 2.7.11.1; Protein kinase, AGC; Kinase, protein; Translation; Protein kinase, Ser/Thr (non-receptor); AGC group; RSK family; RSK subfamily

Chromosomal Location of Human Ortholog: 1p

Cellular Component: nucleoplasm; cytoplasm; spindle; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; caspase inhibitor activity; magnesium ion binding; protein serine/threonine/tyrosine kinase activity; ATP binding

Biological Process: regulation of transcription in response to stress; axon guidance; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; regulation of translation in response to stress; negative regulation of caspase activity; toll-like receptor 3 signaling pathway; signal transduction; cell cycle; positive regulation of cell growth; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; synaptic transmission; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; positive regulation of cell differentiation; toll-like receptor 4 signaling pathway; negative regulation of apoptosis
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Research Articles on RSK1

1. combination antiretroviral therapy increased monocyte/macrophage sensitivity to reactive oxygen species- in HIV(+) individuals by suppressing NRF2-ARE activity via p90RSK-mediated ERK5 S496 phosphorylation, which coordinately elicited senescent phenotypes and proinflammatory responses

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RSK1 antibody

 Reference Product  PubMed Publications
 MAPK1 antibody  >31 publications with RSK1 and MAPK1
 MAPK3 antibody  >28 publications with RSK1 and MAPK3
 RPS6 antibody  >7 publications with RSK1 and RPS6
 TSC2 antibody  >7 publications with RSK1 and TSC2
 TSC1 antibody  >5 publications with RSK1 and TSC1
 CREB1 antibody  >4 publications with RSK1 and CREB1
 EEF2K antibody  >3 publications with RSK1 and EEF2K

Pathways associated with anti-RSK1 antibody

 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies  Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 CREB Phosphorylation Pathway antibodies  CREB Phosphorylation Pathway Diagram
 CREB Phosphorylation Through The Activation Of Ras Pathway antibodies  CREB Phosphorylation Through The Activation Of Ras Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Cytoplasmic Ribosomal Proteins Pathway antibodies  Cytoplasmic Ribosomal Proteins Pathway Diagram

Diseases associated with anti-RSK1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >16 publications with RSK1 and Inflammation
 Nervous System Diseases Antibodies  >13 publications with RSK1 and Nervous System Diseases
 Hypertrophy Antibodies  >11 publications with RSK1 and Hypertrophy
 Disease Models, Animal Antibodies  >11 publications with RSK1 and Disease Models, Animal
 Liver Diseases Antibodies  >10 publications with RSK1 and Liver Diseases
 Breast Neoplasms Antibodies  >8 publications with RSK1 and Breast Neoplasms
 Necrosis Antibodies  >7 publications with RSK1 and Necrosis
 Hyperplasia Antibodies  >6 publications with RSK1 and Hyperplasia
 Adenocarcinoma Antibodies  >5 publications with RSK1 and Adenocarcinoma
 Cell Transformation, Neoplastic Antibodies  >5 publications with RSK1 and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-RSK1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >50 publications with RSK1 and Muscle
 Blood Antibodies  >32 publications with RSK1 and Blood
 Liver Antibodies  >27 publications with RSK1 and Liver
 Brain Antibodies  >25 publications with RSK1 and Brain
 Heart Antibodies  >23 publications with RSK1 and Heart
 Lung Antibodies  >10 publications with RSK1 and Lung
 Vascular Antibodies  >9 publications with RSK1 and Vascular
 Connective Tissue Antibodies  >7 publications with RSK1 and Connective Tissue
 Bone Antibodies  >6 publications with RSK1 and Bone
 Embryonic Tissue Antibodies  >5 publications with RSK1 and Embryonic Tissue
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