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RSPO3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS7004635

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors,… More details

$230 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RSPO3, Polyclonal Antibody
Full Product Name
RSPO3 Antibody
Product Gene Name
Product Synonym Names
R-spondin-3; RSPO3; PWTSR; THSD2; Protein with TSP type-1 repeat; hPWTSR; Roof plate-specific spondin-3; hRspo3; Thrombospondin type-1 domain-containing protein 2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
Affinity Purified
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
Matching Pairs
Unconjugated Antibody: RSPO3 (MBS7004635)
FITC Conjugated Antibody: RSPO3 (MBS1492985)
Matching Pairs
Unconjugated Antibody: RSPO3 (MBS7004635)
HRP Conjugated Antibody: RSPO3 (MBS1493602)
Molecular Weight
33,233 Da
OMIM
610574
3D Structure
ModBase 3D Structure for Q9BXY4
Immunogen
Recombinant human R-spondin-3 protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-RSPO3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-RSPO3 antibody
Recommended dilution: WB:1:500-2000, IHC:1:20-1:200

Western Blot (WB) of anti-RSPO3 antibody

Western blot
All lanes: QTRT1 antibody at 6ug/ml
Lane 1: MCF7 whole cell lysate
Lane 2: PC-3 whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: A431 whole cell lysate
Secondary
Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 24,44 kDa
Observed band size: 44 kDa
Rabbit Anti-RSPO3 Polyclonal Antibody – Cat# MBS7004635

Immunohistochemistry (IHC) of anti-RSPO3 antibody

Immunohistochemistry of paraffin-embedded human testis using MBS7004635 at dilution of 1:100
Rabbit Anti-RSPO3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS7004635

Other Notes

Small volumes of anti-RSPO3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RSPO3 purchase

MBS7004635 is an antibody targeting RSPO3. Also known as RSPO3 Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RSPO3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
R-spondin-3
NCBI Official Synonym Full Names
R-spondin 3
NCBI Official Symbol
RSPO3
NCBI Official Synonym Symbols
PWTSR; THSD2; CRISTIN1
UniProt Protein Name
R-spondin-3
UniProt Synonym Protein Names
Protein with TSP type-1 repeat; hPWTSR; Roof plate-specific spondin-3; hRspo3; Thrombospondin type-1 domain-containing protein 2
Protein Family
UniProt Gene Name
RSPO3
UniProt Synonym Gene Names
PWTSR; THSD2; hPWTSR; hRspo3
UniProt Entry Name
RSPO3_HUMAN
NCBI Protein Information
R-spondin-3

NCBI Summary for RSPO3

This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development. [provided by RefSeq, Jul 2013]
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UniProt Comments for RSPO3

R-spondin-3: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Belongs to the R-spondin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Cell development/differentiation; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6q22.33

Cellular Component: extracellular region

Molecular Function: frizzled binding; heparin binding; receptor binding

Biological Process: angiogenesis; Wnt receptor signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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