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RYR2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9148715

This gene encodes a ryanodine receptor found in cardiac muscle sarcoplasmic reticulum. The encoded protein is one of the components of a calcium channel, composed of a tetramer of the ryanodine receptor proteins and a tetramer of FK506 binding protein 1B… More details

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Product Overview

Online Datasheet in PDF
Product Name
RYR2, Polyclonal Antibody
Full Product Name
RYR2 Rabbit pAb
Product Gene Name
anti-RYR2 antibody
Product Synonym Names
RyR; ARVC2; ARVD2; RYR-2; VTSIP; VACRDS
Immunogen Sequence
ADDRDDYDFL MQTSTYYYSV RIFPGQEPAN VWVGWITSDF HQYDTGFDLD RVRTVTVTLG DEKGKVHESI KRSNCYMVCA GESMSPGQGR NNNGLEIGCV
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Purity/Purification
Affinity purification
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Molecular Weight
Calculated MW: 565kDa
Observed MW: 565kDa
OMIM
180902
3D Structure
ModBase 3D Structure for Q92736
Cross Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1411-1510 of human RYR2 (NP_001026.2).

Product Categories/Family for anti-RYR2 antibody

Cancer; Signal Transduction; Endocrine Metabolism; Neuroscience; Calcium Signaling; Cardiovascular; Hypoxia; Heart; Contractility; Cardiac arrhythmias; Antibodies
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-RYR2 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-RYR2 antibody
WB: 1:500-1:1000
IHC-P: 1:100-1:500
IF/ICC: 1:50-1:200
ELISA: Recommended starting concentration is 1ug/mL.
Please optimize the concentration based on your specific assay requirements.

Western Blot (WB) of anti-RYR2 antibody

Western blot analysis of lysates from Mouse heart using RYR2 Rabbit pAb (MBS9148715) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:90s.
Rabbit Anti-RYR2 Polyclonal Antibody – Cat# MBS9148715

Immunohistochemistry (IHC) of anti-RYR2 antibody

Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using RYR2 Rabbit pAb (MBS9148715) at a dilution of  1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Rabbit Anti-RYR2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9148715

Immunohistochemistry (IHC) of anti-RYR2 antibody

Immunohistochemistry analysis of paraffin-embedded Human heart tissue using RYR2 Rabbit pAb (MBS9148715) at a dilution of  1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Rabbit Anti-RYR2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9148715

Immunofluorescence (IF) of anti-RYR2 antibody

Immunofluorescence analysis of 293T cells using RYR2 Rabbit pAb (MBS9148715) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Rabbit Anti-RYR2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9148715

Other Notes

Small volumes of anti-RYR2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RYR2 purchase

MBS9148715 is an antibody targeting RYR2. Also known as RYR2 Rabbit pAb. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RYR2. It may not necessarily be applicable to this product.

NCBI GI #
112799847
NCBI GeneID
6262
NCBI Accession #
NP_001026.2
UniProt Primary Accession #
Q92736
UniProt Secondary Accession #
Q15411; Q546N8; Q5T3P2
UniProt Related Accession #
Q92736
GenBank Nucleotide #
NM_001035.2
UniProt Protein Name
Ryanodine receptor 2
UniProt Synonym Protein Names
Cardiac muscle ryanodine receptor; Cardiac muscle ryanodine receptor-calcium release channel; Type 2 ryanodine receptor
Protein Family
Ryanodine receptor
UniProt Gene Name
RYR2
UniProt Synonym Gene Names
RYR-2; RyR2; hRYR-2
UniProt Entry Name
RYR2_HUMAN

UniProt Comments for RYR2

RYR2: ryanodine receptor calcium release channel. Regulates communication between transverse-tubules and sarcoplasmic reticulum. Contraction of cardiac muscle is triggered by release of calcium ions from SR following depolarization of T-tubules. Phosphorylation by PKA dissociates FKBP12.6 from RYR2. Two splice isoforms have been described.

Protein type: Transporter, ion channel; Membrane protein, integral; Membrane protein, multi-pass; Channel, ligand-gated; Transporter

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: sarcoplasmic reticulum membrane; protein complex; smooth endoplasmic reticulum; membrane; sarcoplasmic reticulum; plasma membrane; Z disc

Molecular Function: calmodulin binding; calcium-induced calcium release activity; identical protein binding; protein binding; enzyme binding; protein self-association; calcium-release channel activity; calcium channel activity; calcium ion binding; intracellular ligand-gated calcium channel activity; ryanodine-sensitive calcium-release channel activity

Biological Process: positive regulation of sequestering of calcium ion; cytosolic calcium ion homeostasis; regulation of heart rate; calcium-mediated signaling; positive regulation of heart rate; response to redox state; Wnt receptor signaling pathway through beta-catenin; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; response to caffeine; BMP signaling pathway; cellular calcium ion homeostasis; release of sequestered calcium ion into cytosol; calcium ion transport; response to hypoxia; detection of calcium ion; transmembrane transport; cardiac muscle contraction

Disease: Ventricular Tachycardia, Catecholaminergic Polymorphic, 1, With Or Without Atrial Dysfunction And/or Dilated Cardiomyopathy; Arrhythmogenic Right Ventricular Dysplasia, Familial, 2
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RYR2 antibody

 Reference Product  PubMed Publications
 RYR3 antibody  >5076 publications with RYR2 and RYR3
 RYR1 antibody  >5075 publications with RYR2 and RYR1
 ITPR1 antibody  >81 publications with RYR2 and ITPR1
 SRI antibody  >11 publications with RYR2 and SRI
 AKAP6 antibody  >5 publications with RYR2 and AKAP6
 HOMER1 antibody  >4 publications with RYR2 and HOMER1
 CACNA1C antibody  >4 publications with RYR2 and CACNA1C
 FKBP1B antibody  >3 publications with RYR2 and FKBP1B

Pathways associated with anti-RYR2 antibody

 Products by Pathway  Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Circadian Entrainment Pathway antibodies  Circadian Entrainment Pathway Diagram
 Circadian Entrainment Pathway antibodies  Circadian Entrainment Pathway Diagram

Diseases associated with anti-RYR2 antibody

 Disease Name  Pubmed Publications
 VENTRICULAR TACHYCARDIA, CATECHOLAMINERGIC POLYMORPHIC, 1, WITH OR WITHOUT ATRIAL DYSFUNCTION AND/OR DILATED CARDIOMYOPATHY Antibodies  >17421 publications with RYR2 and VENTRICULAR TACHYCARDIA, CATECHOLAMINERGIC POLYMORPHIC, 1, WITH OR WITHOUT ATRIAL DYSFUNCTION AND/OR DILATED CARDIOMYOPATHY
 Heart Diseases Antibodies  >849 publications with RYR2 and Heart Diseases
 Arrhythmias, Cardiac Antibodies  >485 publications with RYR2 and Arrhythmias, Cardiac
 Nervous System Diseases Antibodies  >410 publications with RYR2 and Nervous System Diseases
 Heart Failure Antibodies  >402 publications with RYR2 and Heart Failure
 Tachycardia Antibodies  >307 publications with RYR2 and Tachycardia
 Tachycardia, Ventricular Antibodies  >291 publications with RYR2 and Tachycardia, Ventricular
 Disease Models, Animal Antibodies  >201 publications with RYR2 and Disease Models, Animal
 Cardiomyopathies Antibodies  >139 publications with RYR2 and Cardiomyopathies
 Drug Toxicity Antibodies  >104 publications with RYR2 and Drug Toxicity
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