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SA1/STAG1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758721

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SA1/STAG1, Polyclonal Antibody
Full Product Name
Anti-SA1/STAG1 Antibody Picoband
Product Gene Name
anti-SA1/STAG1 antibody
Product Synonym Names
mediator complex subunit 4; Mediator of RNA polymerase II transcription subunit 4; Activator-recruited cofactor 36 kDa component; ARC36; Mediator complex subunit 4; TRAP/SMCC/PC2 subunit p36 subunit; Vitamin D3 receptor-interacting protein complex 36 kDa component; DRIP36; MED4; ARC36; DRIP36; VDRIP; HSPC126
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
1258
OMIM
604358
3D Structure
ModBase 3D Structure for Q8WVM7
Immunogen
E coli-derived human SA1/STAG1 recombinant protein (Position: Q748-L1224).
Subcellular Localization
Nucleus
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Product Categories/Family for anti-SA1/STAG1 antibody

Primary Antibodies; Epigenetics and Nuclear Signaling; Mediator Complex; Pol II Transcription; Polymerase Associated Factors; Transcription
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-SA1/STAG1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-SA1/STAG1 antibody
WB: 0.25-0.5ug/ml, Human
ICC/IF: 5ug/ml, Human
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-SA1/STAG1 antibody

Figure 1. Western blot analysis of SA1/STAG1 using anti-SA1/STAG1 antibody (MBS1758721).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SA1/STAG1 antigen affinity purified polyclonal antibody (#MBS1758721) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for SA1/STAG1 at approximately 155 kDa. The expected band size for SA1/STAG1 is at 144 kDa.
Rabbit Anti-SA1/STAG1 Polyclonal Antibody – Cat# MBS1758721

Immunofluorescence (IF) of anti-SA1/STAG1 antibody

Figure 2. IF analysis of SA1/STAG1 using anti-SA1/STAG1 antibody (MBS1758721) and anti-Tubulin Alpha antibody (M03989-3).
SA1/STAG1 was detected in immunocytochemical section of T47D cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (#MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-SA1/STAG1 Antibody (MBS1758721) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Rabbit Anti-SA1/STAG1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1758721

Flow Cytometry (FC/FACS) of anti-SA1/STAG1 antibody

Figure 3. Flow Cytometry analysis of U937 cells using anti-SA1/STAG1 antibody (MBS1758721).
Overlay histogram showing U937 cells stained with MBS1758721 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SA1/STAG1 Antibody (MBS1758721, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Rabbit Anti-SA1/STAG1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758721

Product References and Citations for anti-SA1/STAG1 antibody

1. Carramolino, L., Lee, B. C., Zaballos. A., Peled, A., Barthelemy, I., Shav-Tal, Y., Prieto, I., Carmi, P., Gothelf, Y., Gonzalez de Buitrago, G., Aracil, M., Marquez, G., Barbero, J. L., Zipori, D. SA-1, a nuclear protein encoded by one member of a novel gene family: molecular cloning and detection in hemopoietic organs. Gene 195: 151-159, 1997. Note: Erratum: Gene 206: 283 only, 1998. 2. Davidson, I. F., Bauer, B., Goetz, D., Tang, W., Wutz, G., Peters, J. M. DNA loop extrusion by human cohesin. Science 366: 1338-1345, 2019. 3. Lehalle, D., Mosca-Boidron, A.-L., Begtrup, A., Boute-Benejean, O., Charles, P., Cho, M. T., Clarkson, A., Devinsky, O., Duffourd, Y., Duplomb-Jego, L., Gerard, B., Jacquette, A., and 25 others. STAG1 mutations cause a novel cohesinopathy characterised by unspecific syndromic intellectual disability. J. Med. Genet. 54: 479-488, 2017.

Other Notes

Small volumes of anti-SA1/STAG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SA1/STAG1 purchase

MBS1758721 is an antibody targeting SA1/STAG1. Also known as Anti-SA1/STAG1 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SA1/STAG1. It may not necessarily be applicable to this product.

NCBI GI #
209572720
NCBI GeneID
10274
NCBI Accession #
Q8WVM7.3
UniProt Primary Accession #
Q8WVM7
UniProt Secondary Accession #
O00539; Q6P275
UniProt Related Accession #
Q8WVM7
NCBI Official Full Name
Cohesin subunit SA-1
NCBI Official Synonym Full Names
stromal antigen 1
NCBI Official Symbol
STAG1
NCBI Official Synonym Symbols
SA1; SCC3A
UniProt Protein Name
Cohesin subunit SA-1
UniProt Synonym Protein Names
SCC3 homolog 1; Stromal antigen 1
UniProt Gene Name
STAG1
UniProt Synonym Gene Names
SA1
UniProt Entry Name
STAG1_HUMAN
NCBI Protein Information
cohesin subunit SA-1; SCC3 homolog 1; nuclear protein stromal antigen 1

NCBI Summary for SA1/STAG1

This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase. [provided by RefSeq, Jul 2008]
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UniProt Comments for SA1/STAG1

STAG1: Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Belongs to the SCC3 family.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 3q22.3

Cellular Component: nucleoplasm; chromosome; nucleus; cytosol; chromatin; chromosome, pericentric region

Molecular Function: protein binding; chromatin binding

Biological Process: mitosis; cell division; mitotic cell cycle; chromosome segregation
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Research Articles on SA1/STAG1

1. Mutations in STAG1 is associated with acute myeloid leukemia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SA1/STAG1 antibody

 Reference Product  PubMed Publications
 STAG2 antibody  >8 publications with SA1/STAG1 and STAG2
 RAD21 antibody  >6 publications with SA1/STAG1 and RAD21
 SMC3 antibody  >6 publications with SA1/STAG1 and SMC3
 SMC1A antibody  >2 publications with SA1/STAG1 and SMC1A
 ESPL1 antibody  >1 publications with SA1/STAG1 and ESPL1

Pathways associated with anti-SA1/STAG1 antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cohesin Loading Onto Chromatin Pathway antibodies  Cohesin Loading Onto Chromatin Pathway Diagram
 Establishment Of Sister Chromatid Cohesion Pathway antibodies  Establishment Of Sister Chromatid Cohesion Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Meiosis Pathway antibodies  Meiosis Pathway Diagram
 Meiotic Synapsis Pathway antibodies  Meiotic Synapsis Pathway Diagram
 Mitotic Anaphase Pathway antibodies  Mitotic Anaphase Pathway Diagram

Diseases associated with anti-SA1/STAG1 antibody

 Disease Name  Pubmed Publications
 Prostatic Neoplasms Antibodies  >3 publications with SA1/STAG1 and Prostatic Neoplasms
 Kidney Diseases Antibodies  >3 publications with SA1/STAG1 and Kidney Diseases
 Anemia, Hemolytic Antibodies  >1 publications with SA1/STAG1 and Anemia, Hemolytic
 Anemia Antibodies  >1 publications with SA1/STAG1 and Anemia
 Hyperplasia Antibodies  >1 publications with SA1/STAG1 and Hyperplasia
 Ovarian Neoplasms Antibodies  >1 publications with SA1/STAG1 and Ovarian Neoplasms
 Lymphoma Antibodies  >1 publications with SA1/STAG1 and Lymphoma
 Breast Neoplasms Antibodies  >1 publications with SA1/STAG1 and Breast Neoplasms
 Pancreatic Neoplasms Antibodies  >1 publications with SA1/STAG1 and Pancreatic Neoplasms

Organs/Tissues associated with anti-SA1/STAG1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5 publications with SA1/STAG1 and Blood
 Prostate Antibodies  >4 publications with SA1/STAG1 and Prostate
 Bone Antibodies  >3 publications with SA1/STAG1 and Bone
 Kidney Antibodies  >3 publications with SA1/STAG1 and Kidney
 Connective Tissue Antibodies  >2 publications with SA1/STAG1 and Connective Tissue
 Ovary Antibodies  >2 publications with SA1/STAG1 and Ovary
 Nerve Antibodies  >2 publications with SA1/STAG1 and Nerve
 Mouth Antibodies  >1 publications with SA1/STAG1 and Mouth
 Thymus Antibodies  >1 publications with SA1/STAG1 and Thymus
 Embryonic Tissue Antibodies  >1 publications with SA1/STAG1 and Embryonic Tissue
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