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SAFB2 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421672

MBS421672 is an antibody targeting SAFB2. Also known as Goat anti-SAFB2 Antibody. Reactive in Expected from sequence similarity: Human. Host: Goat. More details

$305 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SAFB2, Polyclonal Antibody
Full Product Name
Goat anti-SAFB2 Antibody
Product Gene Name
Product Synonym Names
SAFB2; scaffold attachment factor B2; KIAA0138; SAFB2 antibody; scaffold attachment factor B2 antibody; KIAA0138 antibody
Immunogen Sequence
GEHNQRLEEH QARA
Clonality
Polyclonal
Host
Goat
Species Reactivity
Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
SAFB2 peptide (MBS426041) is used for blocking the activity of SAFB2 antibody (MBS421672)
Molecular Weight
12,169 Da
OMIM
608066
Immunogen
Peptide with sequence C-GEHNQRLEEHQARA, from the internal region of the protein sequence according to NP_055464.1.
Epitope
Internal region
Note
This antibody is not expected to cross-react with SAFB1.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-SAFB2 antibody
Peptide ELISA (EIA)
Application Notes for anti-SAFB2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Preliminary experiments gave bands at approx 75kDa and 60kDa in lysates of HeLa cell lines after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 107kDa according to NP_055464.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Other Notes

Small volumes of anti-SAFB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SAFB2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
scaffold attachment factor B2
NCBI Official Synonym Full Names
scaffold attachment factor B2
NCBI Official Symbol
SAFB2
UniProt Protein Name
Scaffold attachment factor B2
UniProt Gene Name
SAFB2
UniProt Synonym Gene Names
KIAA0138; SAF-B2
UniProt Entry Name
SAFB2_HUMAN
NCBI Protein Information
scaffold attachment factor B2

NCBI Summary for SAFB2

The protein encoded by this gene, along with its paralog (scaffold attachment factor B1), is a repressor of estrogen receptor alpha. The encoded protein binds scaffold/matrix attachment region (S/MAR) DNA and is involved in cell cycle regulation, apoptosis, differentiation, the stress response, and regulation of immune genes. [provided by RefSeq, May 2016]
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UniProt Comments for SAFB2

SAFB2: Binds to scaffold/matrix attachment region (S/MAR) DNA. Can function as an estrogen receptor corepressor and can also inhibit cell proliferation.

Protein type: RNA-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytoplasm; intracellular membrane-bound organelle; nucleoplasm

Molecular Function: DNA binding; identical protein binding; nucleotide binding; protein binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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