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SAR1A Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS624191

SAR1A is involved in transport from the endoplasmic reticulum to the Golgi apparatus (By similarity) and required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of… More details

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Product Overview

Online Datasheet in PDF
Product Name
SAR1A, Polyclonal Antibody
Full Product Name
SAR1A, ID (GTP-binding Protein SAR1a, COPII-associated Small GTPase, SARA1, SARA, SAR1)
Product Gene Name
Product Synonym Names
Anti -SAR1A, ID (GTP-binding Protein SAR1a, COPII-associated Small GTPase, SARA1, SARA, SAR1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human SAR1A.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
22,367 Da
Chromosome Location
Chromosome: 10; NC_000010.10 (71909960..71930285, complement). Location: 10q22.1
3D Structure
ModBase 3D Structure for Q5SQT9
Immunogen
Synthetic peptide selected from the Center region of human SAR1A (KLH).
Applications Tested/Suitable for anti-SAR1A antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-SAR1A antibody
Suitable for use in ELISA, Western Blot, Flow Cytometry, and Immunohistochemistry.
Dilution: Western Blot: 1:100-1:500
ELISA: 1:1,000
Immunohistochemistry: 1:50-1:100
Flow Cytometry: 1:10-1:50

Western Blot (WB) of anti-SAR1A antibody

Western Blot analysis of MBS624191 in 293 (lane 1) and mouse lung tissue(lane 2) lysates (35ug/lane). SAR1A (arrow) was detected using the purified Pab.
Rabbit Anti-SAR1A Polyclonal Antibody – Cat# MBS624191

Western Blot (WB) of anti-SAR1A antibody

Western Blot analysis of SAR1A (arrow) using MBS624191 . 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SAR1A gene.
Rabbit Anti-SAR1A Polyclonal Antibody, Western Blot (WB) – Cat# MBS624191

Immunohistochemistry (IHC) of anti-SAR1A antibody

Formalin-fixed and paraffin-embedded human lung carcinoma with MBS624191, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Rabbit Anti-SAR1A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS624191

Flow Cytometry (FC/FACS) of anti-SAR1A antibody

Flow Cytometric analysis of NCI-H292 cells using MBS624191 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-SAR1A Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS624191

Other Notes

Small volumes of anti-SAR1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SAR1A purchase

MBS624191 is an antibody targeting SAR1A. Also known as SAR1A, ID (GTP-binding Protein SAR1a, COPII-associated Small GTPase, SARA1, SARA, SAR1). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SAR1A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
SAR1 gene homolog A (S. cerevisiae)
NCBI Official Synonym Full Names
SAR1 homolog A (S. cerevisiae)
NCBI Official Symbol
SAR1A
NCBI Official Synonym Symbols
SAR1; Sara; SARA1; masra2
UniProt Protein Name
SAR1 gene homolog A (S. cerevisiae)
Protein Family
UniProt Gene Name
SAR1A
UniProt Entry Name
Q5SQT9_HUMAN
NCBI Protein Information
GTP-binding protein SAR1a; OTTHUMP00000019743; OTTHUMP00000019744; OTTHUMP00000019745; OTTHUMP00000019748; SAR1 gene homolog A; SAR1a gene homolog 1; COPII-associated small GTPase

UniProt Comments for SAR1A

SAR1A: Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES). Belongs to the small GTPase superfamily. SAR1 family.

Protein type: G protein, monomeric, SAR1; G protein; G protein, monomeric

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: Golgi apparatus; sarcoplasmic reticulum

Molecular Function: GTP binding

Biological Process: vesicle-mediated transport; intracellular protein transport
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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