Rabbit Anti-ESPL1 Polyclonal Antibody – Cat# MBS6498943

Separin (ESPL1) Antibody – Rabbit Polyclonal

Name: ESPL1 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6498943
Price: 1025 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6498943

MBS6498943 is an antibody targeting ESPL1. Also known as Separin, Phosphorylated (Ser801) (ESPL1, Separase, Caspase-like Protein ESPL1, Extra Spindle Poles-like 1 Protein, ESP1, KIAA0165) (MaxLight 750). Reactive in Human. Host: Rabbit. More details

$1025 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Separin (ESPL1), Polyclonal Antibody

Full Product Name

Separin, Phosphorylated (Ser801) (ESPL1, Separase, Caspase-like Protein ESPL1, Extra Spindle Poles-like 1 Protein, ESP1, KIAA0165) (MaxLight 750)

Product Gene Name

anti-ESPL1 antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

Purity/Purification

Purified by Immunoaffinity chromatography.

Specificity

Recognizes human ESPL1 when phosphorylated at Ser801.

Preparation and Storage

Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Molecular Weight

233.113

OMIM

604143

3D Structure

ModBase 3D Structure for Q14674

Immunogen

Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser801 of human ESPL1 (KLH).

Conjugate

MaxLight750

Antigen Modification

Phosphorylated

Note

Preservative Free

Product Categories/Family for anti-ESPL1 antibody

Antibodies; Apoptosis; Caspase

Applications Tested/Suitable for anti-ESPL1 antibody

Immunohistochemistry (IHC), FLISA

Application Notes for anti-ESPL1 antibody

IHC: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Product References and Citations for anti-ESPL1 antibody

1. Beausoleil, S.A., et al., Proc. Natl. Acad. Sci. USA 101(33):12130-12135 (2004). 2. Chestukhin, A., et al., Proc. Natl. Acad. Sci. USA 100(8):4574-4579 (2003). 3. Chen, F., et al., J. Biol. Chem. 277(19):16775-16781 (2002). 4. Waizenegger, I., et al., Curr. Biol. 12(16):1368-1378 (2002). 5. Hauf, S., et al., Science 293(5533):1320-1323 (2001).

Other Notes

Small volumes of anti-ESPL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ESPL1. It may not necessarily be applicable to this product.

NCBI GI #

134276943

NCBI GeneID

9700

NCBI Accession #

NP_036423

UniProt Primary Accession #

Q14674

UniProt Related Accession #

Q14674

GenBank Nucleotide #

NP_036423.4

NCBI Official Full Name

separin

NCBI Official Synonym Full Names

extra spindle pole bodies like 1, separase

NCBI Official Symbol

ESPL1

NCBI Official Synonym Symbols

ESP1; SEPA

UniProt Protein Name

Separin

UniProt Synonym Protein Names

Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase

Protein Family

Separin

UniProt Gene Name

ESPL1

UniProt Synonym Gene Names

ESP1; KIAA0165

UniProt Entry Name

ESPL1_HUMAN

NCBI Protein Information

separin

NCBI Summary for ESPL1

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM, Nov 2010]

UniProt Comments for ESPL1

separase: a caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms. Regulated by at least two independent mechanisms. First, it is inactivated via its interaction with securin/PTTG1, which probably covers its active site. Second, phosphorylation inactivates it. It is completely phosphorylated during mitosis and dephosphorylated during anaphase. Two alternatively spliced isoforms have been described.

Protein type: EC 3.4.22.49; Protease

Chromosomal Location of Human Ortholog: 12q

Cellular Component: centrosome; nucleus; cytosol

Molecular Function: protein binding; catalytic activity; cysteine-type peptidase activity

Biological Process: establishment of mitotic spindle localization; homologous chromosome segregation; mitotic sister chromatid segregation; apoptosis; negative regulation of sister chromatid cohesion; positive regulation of mitotic metaphase/anaphase transition; cytokinesis; mitotic cell cycle; proteolysis; meiotic spindle organization and biogenesis

Research Articles on ESPL1

1. LPE motif on the Scc1 substrate is required for rapid and specific cleavage by separase.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ESPL1 antibody

Reference Product	PubMed Publications
PTTG1 antibody	>2 publications with ESPL1 and PTTG1

Pathways associated with anti-ESPL1 antibody

Products by Pathway	Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
M Phase Pathway antibodies	M Phase Pathway Diagram
Mitotic Anaphase Pathway antibodies	Mitotic Anaphase Pathway Diagram
Mitotic Metaphase And Anaphase Pathway antibodies	Mitotic Metaphase And Anaphase Pathway Diagram
Oocyte Meiosis Pathway antibodies	Oocyte Meiosis Pathway Diagram
Oocyte Meiosis Pathway antibodies	Oocyte Meiosis Pathway Diagram

Diseases associated with anti-ESPL1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>6 publications with ESPL1 and Neoplasms
Breast Neoplasms Antibodies	>3 publications with ESPL1 and Breast Neoplasms
Prostatic Neoplasms Antibodies	>1 publications with ESPL1 and Prostatic Neoplasms

Organs/Tissues associated with anti-ESPL1 antibody

Organ/Tissue Name	Pubmed Publications
Bone Marrow Antibodies	>2 publications with ESPL1 and Bone Marrow
Eye Antibodies	>1 publications with ESPL1 and Eye
Prostate Antibodies	>1 publications with ESPL1 and Prostate
Lymph Antibodies	>1 publications with ESPL1 and Lymph
Thymus Antibodies	>1 publications with ESPL1 and Thymus
Mammary Gland Antibodies	>1 publications with ESPL1 and Mammary Gland
Kidney Antibodies	>1 publications with ESPL1 and Kidney
Lymph Node Antibodies	>1 publications with ESPL1 and Lymph Node