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SHARP Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3901641

SHARP functions as a transcriptional repressor by interacting with proteins involved in deacetylation such as the NuRD (nucleosome remodeling) complex. SHARP can also repress transcription by interacting with corepressors such as SMRT/NCoR (silencing mediator… More details

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Product Overview

Online Datasheet in PDF
Product Name
SHARP, Polyclonal Antibody
Full Product Name
Rabbit anti-SHARP Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
SPEN
Product Synonym Names
SHARP; spen homolog, transcriptional regulator; SMART/HDAC1-associated repressor protein; RBM15C; nuclear receptor transcription cofactor; msx2-interacting protein; Msx2 interacting nuclear target (MINT) homolog; MINT; HIAA0929; SPEN homolog; Msx2-interacting protein; spen family transcriptional repressor
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
402,248 Da
OMIM
613484
3D Structure
ModBase 3D Structure for Q96T58
Immunogen
Between 3400 and 3450
Conjugation
Unconjugated

Product Categories/Family for anti-SHARP antibody

Applications Tested/Suitable for anti-SHARP antibody
Immunoprecipitation (IP)
Application Notes for anti-SHARP antibody
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-SHARP antibody

Detection of human SHARP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SHARP antibody MBS3901641 used for IP at 3 ug/mg lysate. SHARP was also immunoprecipitated by rabbit anti-SHARP antibody MBS3901640, which recognizes an upstream epitope. For blotting immunoprecipitated SHARP, MBS3901640 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-SHARP Polyclonal Antibody – Cat# MBS3901641

Other Notes

Small volumes of anti-SHARP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SHARP purchase

MBS3901641 is an antibody targeting SHARP. Also known as Rabbit anti-SHARP Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SHARP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
msx2-interacting protein
NCBI Official Synonym Full Names
spen family transcriptional repressor
NCBI Official Symbol
SPEN
NCBI Official Synonym Symbols
MINT; SHARP; RBM15C; HIAA0929
UniProt Protein Name
Msx2-interacting protein
UniProt Synonym Protein Names
SMART/HDAC1-associated repressor protein; SPEN homolog
Protein Family
UniProt Gene Name
SPEN
UniProt Synonym Gene Names
KIAA0929; MINT; SHARP
UniProt Entry Name
MINT_HUMAN
NCBI Protein Information
msx2-interacting protein

NCBI Summary for SHARP

This gene encodes a hormone inducible transcriptional repressor. Repression of transcription by this gene product can occur through interactions with other repressors, by the recruitment of proteins involved in histone deacetylation, or through sequestration of transcriptional activators. The product of this gene contains a carboxy-terminal domain that permits binding to other corepressor proteins. This domain also permits interaction with members of the NuRD complex, a nucleosome remodeling protein complex that contains deacetylase activity. In addition, this repressor contains several RNA recognition motifs that confer binding to a steroid receptor RNA coactivator; this binding can modulate the activity of both liganded and nonliganded steroid receptors. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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