• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

SLAMF6 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758587

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SLAMF6, Polyclonal Antibody
Full Product Name
Anti-SLAMF6 Antibody Picoband
Product Gene Name
anti-SLAMF6 antibody
Product Synonym Names
Serine/threonine-protein kinase AtPK1/AtPK6; 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Expressed in all tissues.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
24,976 Da
OMIM
606446
3D Structure
ModBase 3D Structure for Q96DU3
Immunogen
E coli-derived human SLAMF6 recombinant protein (Position: Q22-V332). Human SLAMF6 shares 43.1% amino acid (aa) sequence identity with mouse SLAMF6.
Subcellular Localization
Cytoplasm, Nucleus
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross reactivity with other proteins.
Protein Function
Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.

Product Categories/Family for anti-SLAMF6 antibody

Primary Antibodies; Signal Transduction; Signaling Pathway; Small G Proteins
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-SLAMF6 antibody
Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-SLAMF6 antibody
WB: 0.1-0.25ug/ml, Human
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-SLAMF6 antibody

Figure 1. Western blot analysis of SLAMF6 using anti-SLAMF6 antibody (MBS1758587).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Daudi whole cell lysates,
Lane 2: human Ramos whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human MOLT-4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLAMF6 antigen affinity purified polyclonal antibody (#MBS1758587) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for SLAMF6 at approximately 60 kDa. The expected band size for SLAMF6 is at 37 kDa.
Rabbit Anti-SLAMF6 Polyclonal Antibody – Cat# MBS1758587

Flow Cytometry (FC/FACS) of anti-SLAMF6 antibody

Figure 2. Flow Cytometry analysis of JK cells using anti-SLAMF6 antibody (MBS1758587).
Overlay histogram showing JK cells stained with MBS1758587 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLAMF6 Antibody (MBS1758587, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-SLAMF6 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758587

Flow Cytometry (FC/FACS) of anti-SLAMF6 antibody

Figure 3. Flow Cytometry analysis of THP-1 cells using anti-SLAMF6 antibody (MBS1758587).
Overlay histogram showing THP-1 cells stained with MBS1758587 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLAMF6 Antibody (MBS1758587, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-SLAMF6 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758587

Product References and Citations for anti-SLAMF6 antibody

1. Bottino, C., Falco, M., Parolini, S., Marcenaro, E., Augugliaro, R., Sivori, S., Landi, E., Biassoni, R., Notarangelo, L. D., Moretta, L., Moretta, A. NTB-A, a novel SH2D1A-associated surface molecule contributing to the inability of natural killer cells to kill Epstein-Barr virus-infected B cells in X-linked lymphoproliferative disease. J. Exp. Med. 194: 235-246, 2001. Note: Erratum: J. Exp. Med. 194: 705 only, 2001.2. Cao, E., Ramagopal, U. A., Fedorov, A., Federov, E., Yan, Q., Lary, J. W., Cole, J. L., Nathenson, S. G., Almo, S. C. NTB-A receptor crystal structure: insights into homophilic interactions in the signaling lymphocytic activation molecule receptor family. Immunity 25: 559-570, 2006. 3. Flaig, R. M., Stark, S., Watzl, C. Cutting edge: NTB-A activates NK cells via homophilic interaction. J. Immun. 172: 6524-6527, 2004.

Other Notes

Small volumes of anti-SLAMF6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SLAMF6 purchase

MBS1758587 is an antibody targeting SLAMF6. Also known as Anti-SLAMF6 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SLAMF6. It may not necessarily be applicable to this product.

NCBI GI #
296040491
NCBI GeneID
114836
NCBI Accession #
NP_001171643.1
UniProt Primary Accession #
Q96DU3
UniProt Secondary Accession #
Q14CF0; Q5TAS4; Q5TAS6; Q5TAT3; Q96DV0; A6NMW2; B2R8X8
UniProt Related Accession #
Q96DU3
GenBank Nucleotide #
NM_001184714.1
NCBI Official Full Name
SLAM family member 6 isoform 1
NCBI Official Synonym Full Names
SLAM family member 6
NCBI Official Symbol
SLAMF6
NCBI Official Synonym Symbols
KALI; NTBA; CD352; KALIb; Ly108; NTB-A; SF2000
UniProt Protein Name
SLAM family member 6
UniProt Synonym Protein Names
Activating NK receptor; NK-T-B-antigen; NTB-A; CD_antigen: CD352
Protein Family
SLAM family
UniProt Gene Name
SLAMF6
UniProt Synonym Gene Names
KALI; NTB-A
UniProt Entry Name
SLAF6_HUMAN
NCBI Protein Information
SLAM family member 6; NK-T-B-antigen; NTBA receptor; activating NK receptor; natural killer-, T- and B-cell antigen

NCBI Summary for SLAMF6

The protein encoded by this gene is a type I transmembrane protein, belonging to the CD2 subfamily of the immunoglobulin superfamily. This encoded protein is expressed on Natural killer (NK), T, and B lymphocytes. It undergoes tyrosine phosphorylation and associates with the Src homology 2 domain-containing protein (SH2D1A) as well as with SH2 domain-containing phosphatases (SHPs). It functions as a coreceptor in the process of NK cell activation. It can also mediate inhibitory signals in NK cells from X-linked lymphoproliferative patients. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, May 2010]
Show more

UniProt Comments for SLAMF6

SLAMF6: Triggers cytolytic activity only in natural killer cells (NK) expressing high surface densities of natural cytotoxicity receptors. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1q23.2

Cellular Component: integral to membrane; plasma membrane

Molecular Function: receptor activity
Show more

Research Articles on SLAMF6

1. our data reveal how SAP nucleates a previously unknown signaling complex involving NTB-A and LCK to potentiate restimulation-induced cell death of activated human T cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SLAMF6 antibody

 Reference Product  PubMed Publications
 CD48 antibody  >3 publications with SLAMF6 and CD48
 SH2D1A antibody  >2 publications with SLAMF6 and SH2D1A
 PTPN11 antibody  >1 publications with SLAMF6 and PTPN11

Diseases associated with anti-SLAMF6 antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >2 publications with SLAMF6 and Necrosis
 Lung Diseases Antibodies  >1 publications with SLAMF6 and Lung Diseases
 Lymphoma Antibodies  >1 publications with SLAMF6 and Lymphoma
 Lung Injury Antibodies  >1 publications with SLAMF6 and Lung Injury
 Inflammation Antibodies  >1 publications with SLAMF6 and Inflammation

Organs/Tissues associated with anti-SLAMF6 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >16 publications with SLAMF6 and Blood
 Bone Marrow Antibodies  >6 publications with SLAMF6 and Bone Marrow
 Thymus Antibodies  >3 publications with SLAMF6 and Thymus
 Lung Antibodies  >2 publications with SLAMF6 and Lung
 Pharynx Antibodies  >2 publications with SLAMF6 and Pharynx
 Spleen Antibodies  >2 publications with SLAMF6 and Spleen
 Lymph Node Antibodies  >1 publications with SLAMF6 and Lymph Node
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000