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SLC12A5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS645904

MBS645904 is an antibody targeting SLC12A5. Also known as SLC12A5, NT (SLC12A5, KCC2, KIAA1176, Solute carrier family 12 member 5, Electroneutral potassium-chloride cotransporter 2, K-Cl cotransporter 2, Neuronal K-Cl cotransporter). Reactive in Human. Host:… More details

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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SLC12A5, Polyclonal Antibody
Full Product Name
SLC12A5, NT (SLC12A5, KCC2, KIAA1176, Solute carrier family 12 member 5, Electroneutral potassium-chloride cotransporter 2, K-Cl cotransporter 2, Neuronal K-Cl cotransporter)
Product Gene Name
anti-SLC12A5 antibody
Product Synonym Names
Anti -SLC12A5, NT (SLC12A5, KCC2, KIAA1176, Solute carrier family 12 member 5, Electroneutral potassium-chloride cotransporter 2, K-Cl cotransporter 2, Neuronal K-Cl cotransporter)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
126,184 Da
Chromosome Location
Chromosome: 20; NC_000020.10 (44650329..44688789). Location: 20q13.12
OMIM
606726
3D Structure
ModBase 3D Structure for Q9H2X9
Immunogen
SLC12A5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-41 amino acids from the N-terminal region of human SLC12A5.

Product Categories/Family for anti-SLC12A5 antibody

Antibodies; Abs to KIAA; orf
Applications Tested/Suitable for anti-SLC12A5 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-SLC12A5 antibody
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100

Western Blot (WB) of anti-SLC12A5 antibody

SLC12A5 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SLC12A5 antibody detected the SLC12A5 protein (arrow).
Rabbit Anti-SLC12A5 Polyclonal Antibody – Cat# MBS645904

Immunohistochemistry (IHC) of anti-SLC12A5 antibody

SLC12A5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SLC12A5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-SLC12A5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS645904

Other Notes

Small volumes of anti-SLC12A5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SLC12A5. It may not necessarily be applicable to this product.

NCBI GI #
198041678
NCBI GeneID
57468
NCBI Accession #
NP_001128243.1
UniProt Primary Accession #
Q9H2X9
UniProt Secondary Accession #
Q5VZ41; Q9H4Z0; Q9ULP4; A2RTX2
UniProt Related Accession #
Q9H2X9
GenBank Nucleotide #
NM_001134771.1
NCBI Official Full Name
solute carrier family 12 member 5 isoform 1
NCBI Official Synonym Full Names
solute carrier family 12 (potassium/chloride transporter), member 5
NCBI Official Symbol
SLC12A5
NCBI Official Synonym Symbols
KCC2
UniProt Protein Name
Solute carrier family 12 member 5
UniProt Synonym Protein Names
Electroneutral potassium-chloride cotransporter 2; K-Cl cotransporter 2; hKCC2; Neuronal K-Cl cotransporter
Protein Family
Solute carrier family
UniProt Gene Name
SLC12A5
UniProt Synonym Gene Names
KCC2; KIAA1176; hKCC2
UniProt Entry Name
S12A5_HUMAN
NCBI Protein Information
solute carrier family 12 member 5; hKCC2; K-Cl cotransporter 2; neuronal K-Cl cotransporter; erythroid K-Cl cotransporter 2; electroneutral potassium-chloride cotransporter 2

NCBI Summary for SLC12A5

K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Sep 2008]
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UniProt Comments for SLC12A5

Function: Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl- homeostasis in neurons.

Enzyme regulation: Inhibited by WNK3. Ref.6

Subunit structure: Homomultimer and heteromultimer with other K-Cl cotransporters. Interacts with AP2A1

By similarity.

Subcellular location: Membrane; Multi-pass membrane protein.

Tissue specificity: Brain specific. Detected in neuronal cells.

Miscellaneous: Inhibited by furosemide and bumetanide.

Sequence similarities: Belongs to the SLC12A transporter family.
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Research Articles on SLC12A5

1. BPA can disrupt Kcc2 gene expression through epigenetic mechanisms

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SLC12A5 antibody

 Products by Pathway  Pathway Diagram
 Cation-coupled Chloride Cotransporters Pathway antibodies  Cation-coupled Chloride Cotransporters Pathway Diagram
 GABAergic Synapse Pathway antibodies  GABAergic Synapse Pathway Diagram
 GABAergic Synapse Pathway antibodies  GABAergic Synapse Pathway Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway antibodies  Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway Diagram

Diseases associated with anti-SLC12A5 antibody

 Disease Name  Pubmed Publications
 Growth Disorders Antibodies  >2 publications with SLC12A5 and Growth Disorders
 Cognition Disorders Antibodies  >2 publications with SLC12A5 and Cognition Disorders
 Epilepsy Antibodies  >2 publications with SLC12A5 and Epilepsy
 Spinal Cord Diseases Antibodies  >1 publications with SLC12A5 and Spinal Cord Diseases
 Necrosis Antibodies  >1 publications with SLC12A5 and Necrosis
 Neoplasms Antibodies  >1 publications with SLC12A5 and Neoplasms
 Hypertension Antibodies  >1 publications with SLC12A5 and Hypertension
 Liver Diseases Antibodies  >1 publications with SLC12A5 and Liver Diseases
 Kidney Diseases Antibodies  >1 publications with SLC12A5 and Kidney Diseases
 Seizures Antibodies  >1 publications with SLC12A5 and Seizures

Organs/Tissues associated with anti-SLC12A5 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >8 publications with SLC12A5 and Brain
 Kidney Antibodies  >3 publications with SLC12A5 and Kidney
 Muscle Antibodies  >1 publications with SLC12A5 and Muscle
 Lung Antibodies  >1 publications with SLC12A5 and Lung
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