Rabbit Anti-SLP2/SYTL2 Polyclonal Antibody – Cat# MBS1758905

Figure 1. Western blot analysis of SLP2/SYTL2 using anti-SLP2/SYTL2 antibody (MBS1758905).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLP2/SYTL2 antigen affinity purified polyclonal antibody (#MBS1758905) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for SLP2/SYTL2 at approximately 120-130 kDa. The expected band size for SLP2/SYTL2 is at 105 kDa.

SLP2/SYTL2 Antibody – Rabbit Polyclonal

Name: SLP2/SYTL2 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1758905
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758905

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SLP2/SYTL2, Polyclonal Antibody

Full Product Name

Anti-SLP2/SYTL2 Antibody Picoband

Product Gene Name

anti-SLP2/SYTL2 antibody

Product Synonym Names

neuropeptides B and W receptor 1; Neuropeptides B/W receptor type 1; G-protein coupled receptor 7; NPBWR1; GPR7

Clonality

Polyclonal

Isotype

Rabbit IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purity/Purification

Immunogen affinity purified.

Specificity

Found in cerebellum and frontal cortex. Detected at high levels in hippocampus, amygdala and trachea; at moderate levels in fetal brain, pituitary gland and prostate. Not in caudate, accumbens, kidney or liver. Also detected at high levels in lung carcinoma.

Preparation and Storage

At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.

Molecular Weight

84,651 Da

OMIM

EU035829 mRNA

3D Structure

ModBase 3D Structure for Q9HCH5

Immunogen

E coli-derived human SLP2/SYTL2 recombinant protein (Position: Q522-K934).

Subcellular Localization

Cell membrane. Multi-pass membrane protein

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.

Cross Reactivity

No cross-reactivity with other proteins.

Protein Function

Interacts specifically with a number of opioid ligands. Receptor for neuropeptides B and W, which may be involved in neuroendocrine system regulation, food intake and the organization of other signals. Has a higher affinity for neuropeptide B.

Product Categories/Family for anti-SLP2/SYTL2 antibody

Primary Antibodies; Immune System Diseases; Immunology; Protein Trafficking; Signal Transduction; Signaling Pathway

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-SLP2/SYTL2 antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-SLP2/SYTL2 antibody

WB: 0.25-0.5ug/ml, Human
ELISA: 0.1-0.5ug/ml

Product References and Citations for anti-SLP2/SYTL2 antibody

1. Kuroda, T. S., Fukuda, M. Rab27A-binding protein Slp2-a is required for peripheral melanosome distribution and elongated cell shape in melanocytes. Nature Cell Biol. 6: 1195-1203, 2004. 2. Kuroda, T. S., Fukuda, M., Ariga, H., Mikoshiba, K. The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem. 277: 9212-9218, 2002.3. Menasche, G., Menager, M. M., Lefebvre, J. M., Deutsch, E., Athman, R., Lambert, N., Mahlaoui, N., Court, M., Garin, J., Fischer, A., de Saint Basile, G. A newly identified isoform of Slp2a associates with Rab27a in cytotoxic T cells and participates in cytotoxic granule selection. Blood 112: 5052-5062, 2008.

Other Notes

Small volumes of anti-SLP2/SYTL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SLP2/SYTL2 purchase

MBS1758905 is an antibody targeting SLP2/SYTL2. Also known as Anti-SLP2/SYTL2 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SLP2/SYTL2. It may not necessarily be applicable to this product.

NCBI GI #

244790013

NCBI GeneID

54843

NCBI Accession #

NP_001156423.1

UniProt Primary Accession #

Q9HCH5

UniProt Secondary Accession #

Q2YDA7; Q6TV07; Q6ZN59; Q6ZVC5; B3KRS3; B4DJT5; B4DKW3; B4DQ26; B7SA85; B7ZLX6; B7ZLX7

UniProt Related Accession #

Q9HCH5

GenBank Nucleotide #

NM_001162951.2

NCBI Official Full Name

synaptotagmin-like protein 2 isoform g

NCBI Official Synonym Full Names

synaptotagmin like 2

NCBI Official Symbol

SYTL2

NCBI Official Synonym Symbols

EXO4; SLP2; SLP2A; SGA72M; CHR11SYT; PPP1R151

UniProt Protein Name

Synaptotagmin-like protein 2

UniProt Synonym Protein Names

Breast cancer-associated antigen SGA-72M; Exophilin-4

UniProt Gene Name

SYTL2

UniProt Synonym Gene Names

KIAA1597; SGA72M; SLP2; SLP2A

NCBI Protein Information

synaptotagmin-like protein 2

NCBI Summary for SLP2/SYTL2

The protein encoded by this gene is a synaptotagmin-like protein (SLP) that belongs to a C2 domain-containing protein family. The SLP homology domain (SHD) of this protein has been shown to specifically bind the GTP-bound form of Ras-related protein Rab-27A (RAB27A). This protein plays a role in RAB27A-dependent vesicle trafficking and controls melanosome distribution in the cell periphery. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jun 2009]

UniProt Comments for SLP2/SYTL2

Isoform 1 acts as a RAB27A effector protein and plays a role in cytotoxic granule exocytosis in lymphocytes. It is required for cytotoxic granule docking at the immunologic synapse. Isoform 4 binds phosphatidylserine (PS) and phosphatidylinositol-4,5-bisphosphate (PIP2) and promotes the recruitment of glucagon-containing granules to the cell membrane in pancreatic alpha cells. Binding to PS is inhibited by Ca2+ while binding to PIP2 is Ca2+ insensitive.

Research Articles on SLP2/SYTL2

1. SLP2 may play an important role in tumorigenesis of esophageal squamous cell carcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SLP2/SYTL2 antibody

Reference Product	PubMed Publications
RAB27A antibody	>13 publications with SLP2/SYTL2 and RAB27A
MLPH antibody	>3 publications with SLP2/SYTL2 and MLPH
RAB27B antibody	>3 publications with SLP2/SYTL2 and RAB27B
MYRIP antibody	>1 publications with SLP2/SYTL2 and MYRIP
CLDN2 antibody	>1 publications with SLP2/SYTL2 and CLDN2

Pathways associated with anti-SLP2/SYTL2 antibody

Products by Pathway	Pathway Diagram
Deregulation Of Rab And Rab Effector Genes In Bladder Cancer Pathway antibodies	Deregulation Of Rab And Rab Effector Genes In Bladder Cancer Pathway Diagram

Diseases associated with anti-SLP2/SYTL2 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>2 publications with SLP2/SYTL2 and Inflammation
Cell Transformation, Neoplastic Antibodies	>1 publications with SLP2/SYTL2 and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-SLP2/SYTL2 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>3 publications with SLP2/SYTL2 and Muscle
Brain Antibodies	>3 publications with SLP2/SYTL2 and Brain
Uncharacterized Tissue Antibodies	>2 publications with SLP2/SYTL2 and Uncharacterized Tissue
Kidney Antibodies	>2 publications with SLP2/SYTL2 and Kidney
Stomach Antibodies	>1 publications with SLP2/SYTL2 and Stomach
Pancreas Antibodies	>1 publications with SLP2/SYTL2 and Pancreas
Bladder Antibodies	>1 publications with SLP2/SYTL2 and Bladder