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SMAD9 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6348806

The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different… More details

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Product Overview

Online Datasheet in PDF
Product Name
SMAD9, Polyclonal Antibody
Full Product Name
SMAD9, ID (SMAD9, MADH6, MADH9, Mothers against decapentaplegic homolog 9, Madh6, SMAD family member 9) (FITC)
Product Gene Name
anti-SMAD9 antibody
Product Synonym Names
SMAD9; MADH6; MADH9; Mothers against decapentaplegic homolog 9; Madh6; SMAD family member 9
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
48,640 Da
OMIM
gene 615342
3D Structure
ModBase 3D Structure for O15198
Immunogen
SMAD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 199-228 amino acids from the Central region of human SMAD9.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-SMAD9 antibody

Antibodies; Smad Proteins
Applications Tested/Suitable for anti-SMAD9 antibody
Western Blot (WB), FLISA
Application Notes for anti-SMAD9 antibody
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-SMAD9 antibody

SMAD9 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SMAD9 antibody detected the SMAD9 protein (arrow).
Rabbit Anti-SMAD9 Polyclonal Antibody – Cat# MBS6348806

Immunofluorescence (IF) of anti-SMAD9 antibody

Fluorescent confocal image of MCF-7 cell stained with SMAD9 Antibody (Center). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD9 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).SMAD9 immunoreactivity is localized to cytoplasm and nucleus significantly.
Rabbit Anti-SMAD9 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6348806

Product References and Citations for anti-SMAD9 antibody

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Liu, Y., et al. FASEB J. 23(7):2299-2306(2009). Su, D., et al. J. Biol. Chem. 284(18):12153-12164(2009). Shintani, M., et al. J. Med. Genet. 46(5):331-337(2009). Hoover, L.L., et al. Sci Signal 1 (46), PE48 (2008).

Other Notes

Small volumes of anti-SMAD9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SMAD9 purchase

MBS6348806 is an antibody targeting SMAD9. Also known as SMAD9, ID (SMAD9, MADH6, MADH9, Mothers against decapentaplegic homolog 9, Madh6, SMAD family member 9) (FITC). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SMAD9. It may not necessarily be applicable to this product.

NCBI GI #
5174519
NCBI GeneID
4093
NCBI Accession #
NP_005896.1
NCBI Related Accession #
NP_001120689.1
UniProt Primary Accession #
O15198
UniProt Secondary Accession #
O14989; Q5TBA1; A2A2Y6
UniProt Related Accession #
O15198
GenBank Nucleotide #
NM_005905.5
NCBI Official Full Name
mothers against decapentaplegic homolog 9 isoform b
NCBI Official Synonym Full Names
SMAD family member 9
NCBI Official Symbol
SMAD9
NCBI Official Synonym Symbols
PPH2; MADH6; MADH9; SMAD8; SMAD8A; SMAD8B; SMAD8/9
UniProt Protein Name
Mothers against decapentaplegic homolog 9
UniProt Synonym Protein Names
Madh6; SMAD family member 9; SMAD 9; Smad9
Protein Family
Mothers against decapentaplegic
UniProt Gene Name
SMAD9
UniProt Synonym Gene Names
MADH6; MADH9; MAD homolog 9; Mothers against DPP homolog 9; SMAD 9; Smad9
UniProt Entry Name
SMAD9_HUMAN
NCBI Protein Information
mothers against decapentaplegic homolog 9

UniProt Comments for SMAD9

Smad8: transcription factor phosphorylated and activated by bone morphogenetic protein receptor kinases. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where they regulate transcription of target genes. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. Two alternatively spliced products have been described.

Protein type: Transcription, coactivator/corepressor; DNA-binding

Chromosomal Location of Human Ortholog: 13q12-q14

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; intracellular; cytosol; nucleus

Molecular Function: protein binding; DNA binding; metal ion binding; transcription factor activity; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity

Biological Process: BMP signaling pathway; Mullerian duct regression; transcription, DNA-dependent; ureteric bud development; positive regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; cartilage development; midbrain development; response to hypoxia; positive regulation of cell differentiation; protein amino acid phosphorylation; hindbrain development

Disease: Pulmonary Hypertension, Primary, 2
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Research Articles on SMAD9

1. Case Report: hamartomatous olyposis and gastrointestinal ganglioneuromas with SMAD9 germline mutation that reduces PTEN expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SMAD9 antibody

 Reference Product  PubMed Publications
 BMPR2 antibody  >15 publications with SMAD9 and BMPR2
 BMP2 antibody  >13 publications with SMAD9 and BMP2
 SMAD4 antibody  >13 publications with SMAD9 and SMAD4
 BMP4 antibody  >10 publications with SMAD9 and BMP4
 BMPR1A antibody  >9 publications with SMAD9 and BMPR1A
 BMPR1B antibody  >4 publications with SMAD9 and BMPR1B
 BMP7 antibody  >3 publications with SMAD9 and BMP7
 ACVRL1 antibody  >1 publications with SMAD9 and ACVRL1
 ACVR1 antibody  >1 publications with SMAD9 and ACVR1
 GDF2 antibody  >1 publications with SMAD9 and GDF2

Pathways associated with anti-SMAD9 antibody

 Products by Pathway  Pathway Diagram
 ALK1 Signaling Events Pathway antibodies  ALK1 Signaling Events Pathway Diagram
 ALK2 Signaling Events Pathway antibodies  ALK2 Signaling Events Pathway Diagram
 BMP Receptor Signaling Pathway antibodies  BMP Receptor Signaling Pathway Diagram
 BMP Signaling Pathway antibodies  BMP Signaling Pathway Diagram
 BMP Signaling Pathway antibodies  BMP Signaling Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By BMP Pathway antibodies  Signaling By BMP Pathway Diagram
 Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies  Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
 Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies  Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
 TGF Beta Signaling Pathway antibodies  TGF Beta Signaling Pathway Diagram

Diseases associated with anti-SMAD9 antibody

 Disease Name  Pubmed Publications
 Hypertension Antibodies  >6 publications with SMAD9 and Hypertension
 Hypertension, Pulmonary Antibodies  >6 publications with SMAD9 and Hypertension, Pulmonary
 Lung Diseases Antibodies  >5 publications with SMAD9 and Lung Diseases
 Carcinoma Antibodies  >2 publications with SMAD9 and Carcinoma
 Inflammation Antibodies  >2 publications with SMAD9 and Inflammation
 Heart Defects, Congenital Antibodies  >1 publications with SMAD9 and Heart Defects, Congenital
 Atherosclerosis Antibodies  >1 publications with SMAD9 and Atherosclerosis
 Fibrosis Antibodies  >1 publications with SMAD9 and Fibrosis
 Edema Antibodies  >1 publications with SMAD9 and Edema
 Brain Diseases Antibodies  >1 publications with SMAD9 and Brain Diseases

Organs/Tissues associated with anti-SMAD9 antibody

 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >8 publications with SMAD9 and Embryonic Tissue
 Muscle Antibodies  >6 publications with SMAD9 and Muscle
 Liver Antibodies  >5 publications with SMAD9 and Liver
 Heart Antibodies  >4 publications with SMAD9 and Heart
 Lung Antibodies  >3 publications with SMAD9 and Lung
 Brain Antibodies  >3 publications with SMAD9 and Brain
 Kidney Antibodies  >2 publications with SMAD9 and Kidney
 Uterus Antibodies  >2 publications with SMAD9 and Uterus
 Eye Antibodies  >2 publications with SMAD9 and Eye
 Stomach Antibodies  >1 publications with SMAD9 and Stomach
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