Rabbit Anti-SMEK2 Polyclonal Antibody – Cat# MBS6348947

SMEK2 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SMEK2 antibody detected the SMEK2 protein (arrow).

SMEK2 Antibody – Rabbit Polyclonal

Name: SMEK2 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6348947
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6348947

Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. More details

$1060 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SMEK2, Polyclonal Antibody

Full Product Name

SMEK2, CT (SMEK2, KIAA1387, PP4R3B, PPP4R3B, Serine/threonine-protein phosphatase 4 regulatory subunit 3B, SMEK homolog 2) (APC)

Product Gene Name

anti-SMEK2 antibody

Product Synonym Names

SMEK2; KIAA1387; PP4R3B; PPP4R3B; Serine/threonine-protein phosphatase 4 regulatory subunit 3B; SMEK homolog 2

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

Purity/Purification

Purified by Protein A Affinity Chromatography.

Preparation and Storage

Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Molecular Weight

84 kDa

OMIM

610352

3D Structure

ModBase 3D Structure for Q5MIZ7

Immunogen

SMEK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 719-748 amino acids from the C-terminal region of human SMEK2.

Conjugate

APC

Note

Preservative Free

Special Handling

Light sensitive

Product Categories/Family for anti-SMEK2 antibody

Antibodies; Enzymes; Phosphatase

Applications Tested/Suitable for anti-SMEK2 antibody

Western Blot (WB), FLISA

Application Notes for anti-SMEK2 antibody

WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Product References and Citations for anti-SMEK2 antibody

Chowdhury, D., et al. Mol. Cell 31(1):33-46(2008). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006). Gingras, A.C., et al. Mol. Cell Proteomics 4(11):1725-1740(2005). Mendoza, M.C., et al. Mol. Cell. Biol. 25(17):7839-7853(2005).

Other Notes

Small volumes of anti-SMEK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SMEK2 purchase

MBS6348947 is an antibody targeting SMEK2. Also known as SMEK2, CT (SMEK2, KIAA1387, PP4R3B, PPP4R3B, Serine/threonine-protein phosphatase 4 regulatory subunit 3B, SMEK homolog 2) (APC). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SMEK2. It may not necessarily be applicable to this product.

NCBI GI #

334302843

NCBI GeneID

57223

NCBI Accession #

NP_065196.1

NCBI Related Accession #

NP_001116436.1

UniProt Primary Accession #

Q5MIZ7

UniProt Related Accession #

Q5MIZ7

GenBank Nucleotide #

NM_020463.3

NCBI Official Full Name

Serine/threonine-protein phosphatase 4 regulatory subunit 3B

NCBI Official Synonym Full Names

protein phosphatase 4 regulatory subunit 3B

NCBI Official Symbol

PPP4R3B

NCBI Official Synonym Symbols

PSY2; smk1; FLFL2; SMEK2; PP4R3B

UniProt Protein Name

Serine/threonine-protein phosphatase 4 regulatory subunit 3B

UniProt Synonym Protein Names

SMEK homolog 2

UniProt Gene Name

SMEK2

UniProt Synonym Gene Names

KIAA1387; PP4R3B; PPP4R3B

UniProt Entry Name

P4R3B_HUMAN

NCBI Protein Information

serine/threonine-protein phosphatase 4 regulatory subunit 3B

UniProt Comments for SMEK2

SMEK2: Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Belongs to the SMEK family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein phosphatase, regulatory subunit; Cell cycle regulation

Chromosomal Location of Human Ortholog: 2p16.1

Cellular Component: nucleoplasm; cytoplasm; microtubule organizing center

Biological Process: positive regulation of gluconeogenesis; regulation of lipid metabolic process; protein amino acid dephosphorylation

Research Articles on SMEK2

1. A three-protein PP4 phosphatase complex in mammalian cells, containing PP4C, PP4R2, and PP4R3beta, specifically dephosphorylates ATR-mediated gamma-H2AX generated during DNA replication.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SMEK2 antibody

Products by Pathway	Pathway Diagram
Integrated Breast Cancer Pathway antibodies	Integrated Breast Cancer Pathway Diagram

Diseases associated with anti-SMEK2 antibody

Disease Name	Pubmed Publications
Fatty Liver Antibodies	>1 publications with SMEK2 and Fatty Liver

Organs/Tissues associated with anti-SMEK2 antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>3 publications with SMEK2 and Liver
Blood Antibodies	>1 publications with SMEK2 and Blood
Vascular Antibodies	>1 publications with SMEK2 and Vascular