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SMPD1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6349028

MBS6349028 is an antibody targeting SMPD1. Also known as SMPD1, CT (SMPD1, ASM, Sphingomyelin phosphodiesterase, Acid sphingomyelinase) (MaxLight 405). Reactive in Human. Host: Rabbit. More details

$1025 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SMPD1, Polyclonal Antibody
Full Product Name
SMPD1, CT (SMPD1, ASM, Sphingomyelin phosphodiesterase, Acid sphingomyelinase) (MaxLight 405)
Product Gene Name
anti-SMPD1 antibody
Product Synonym Names
SMPD1; ASM; Sphingomyelin phosphodiesterase; Acid sphingomyelinase
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Specificity
Recognizes human SMPD1.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
629
Chromosome Location
Chromosome: 11; NC_000011.9 (6411644..6416228). Location: 11p15.4-p15.1
OMIM
257200
3D Structure
ModBase 3D Structure for P17405
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 391-419 in the C-terminal region of human SMPD1. Species sequence homology: bovine
Conjugate
MaxLight405
Note
Preservative Free

Product Categories/Family for anti-SMPD1 antibody

Antibodies; Enzymes; Phosphodiesterase
Applications Tested/Suitable for anti-SMPD1 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
Application Notes for anti-SMPD1 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-SMPD1 antibody

Western blot analysis of K562 cell line lysates (35ug/lane) usingMBS641963. This demonstrates MBS641963 detected the SMPD1 protein (arrow).
Rabbit Anti-SMPD1 Polyclonal Antibody – Cat# MBS6349028

Immunohistochemistry (IHC) of anti-SMPD1 antibody

Immunohistochemistry analysis of MBS641963 in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS641963 for immunohistochemistry.
Rabbit Anti-SMPD1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6349028

Testing Data of anti-SMPD1 antibody

All lanes : MBS641963 (1:4000) Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: A431 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 70 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-SMPD1 Polyclonal Antibody, Testing Data – Cat# MBS6349028

Product References and Citations for anti-SMPD1 antibody

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Desnick, J.P., et al. Mol. Med. 16 (7-8), 316-321 (2010): Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Sleat, D.E., et al. Mol. Cell Proteomics 5(4):686-701(2006).

Other Notes

Small volumes of anti-SMPD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SMPD1. It may not necessarily be applicable to this product.

NCBI GI #
56117840
NCBI GeneID
6609
NCBI Accession #
NP_000534.3
UniProt Primary Accession #
P17405
UniProt Secondary Accession #
P17406; Q13811; Q16837; Q16841; A8K8M3; E9PKS3
UniProt Related Accession #
P17405
GenBank Nucleotide #
NM_000543.4
NCBI Official Full Name
sphingomyelin phosphodiesterase isoform 1
NCBI Official Synonym Full Names
sphingomyelin phosphodiesterase 1, acid lysosomal
NCBI Official Symbol
SMPD1
NCBI Official Synonym Symbols
ASM; NPD; ASMASE
UniProt Protein Name
Sphingomyelin phosphodiesterase
UniProt Synonym Protein Names
Acid sphingomyelinase
Protein Family
Sphingomyelin phosphodiesterase
UniProt Gene Name
SMPD1
UniProt Synonym Gene Names
ASM; aSMase
UniProt Entry Name
ASM_HUMAN
NCBI Protein Information
sphingomyelin phosphodiesterase; acid sphingomyelinase

UniProt Comments for SMPD1

SMPD1: Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. Monomer. Belongs to the acid sphingomyelinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - sphingolipid; EC 3.1.4.12; Phosphodiesterase

Chromosomal Location of Human Ortholog: 11p15.4-p15.1

Cellular Component: extracellular space; lysosomal lumen; lysosome; plasma membrane; endosome; lamellar body

Molecular Function: protein binding; sphingomyelin phosphodiesterase activity; hydrolase activity, acting on glycosyl bonds

Biological Process: response to drug; negative regulation of MAP kinase activity; nervous system development; sphingomyelin metabolic process; sphingolipid metabolic process; positive regulation of apoptosis; ceramide biosynthetic process; glycosphingolipid metabolic process; sphingomyelin catabolic process; response to cocaine; signal transduction; positive regulation of protein amino acid dephosphorylation

Disease: Niemann-pick Disease, Type B; Niemann-pick Disease, Type A
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SMPD1 antibody

 Reference Product  PubMed Publications
 GBA antibody  >1 publications with SMPD1 and GBA
 SMPD2 antibody  >1 publications with SMPD1 and SMPD2

Pathways associated with anti-SMPD1 antibody

 Products by Pathway  Pathway Diagram
 Ceramide Signaling Pathway antibodies  Ceramide Signaling Pathway Diagram
 FAS (CD95) Signaling Pathway antibodies  FAS (CD95) Signaling Pathway Diagram
 Glycosphingolipid Metabolism Pathway antibodies  Glycosphingolipid Metabolism Pathway Diagram
 IL2 Signaling Events Mediated By PI3K Pathway antibodies  IL2 Signaling Events Mediated By PI3K Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Ovarian Infertility Genes Pathway antibodies  Ovarian Infertility Genes Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram

Diseases associated with anti-SMPD1 antibody

 Disease Name  Pubmed Publications
 Niemann-Pick Diseases Antibodies  >30 publications with SMPD1 and Niemann-Pick Diseases
 Niemann-Pick Disease, Type B Antibodies  >10 publications with SMPD1 and Niemann-Pick Disease, Type B
 Neoplasms Antibodies  >4 publications with SMPD1 and Neoplasms
 Inflammation Antibodies  >4 publications with SMPD1 and Inflammation
 Disease Models, Animal Antibodies  >3 publications with SMPD1 and Disease Models, Animal
 Niemann-Pick Disease, Type A Antibodies  >3 publications with SMPD1 and Niemann-Pick Disease, Type A
 Necrosis Antibodies  >2 publications with SMPD1 and Necrosis
 Liver Diseases Antibodies  >1 publications with SMPD1 and Liver Diseases
 Hepatomegaly Antibodies  >1 publications with SMPD1 and Hepatomegaly
 Fibrosis Antibodies  >1 publications with SMPD1 and Fibrosis

Organs/Tissues associated with anti-SMPD1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >10 publications with SMPD1 and Blood
 Brain Antibodies  >9 publications with SMPD1 and Brain
 Liver Antibodies  >6 publications with SMPD1 and Liver
 Skin Antibodies  >5 publications with SMPD1 and Skin
 Bone Antibodies  >5 publications with SMPD1 and Bone
 Vascular Antibodies  >3 publications with SMPD1 and Vascular
 Lung Antibodies  >3 publications with SMPD1 and Lung
 Adipose Tissue Antibodies  >2 publications with SMPD1 and Adipose Tissue
 Nerve Antibodies  >2 publications with SMPD1 and Nerve
 Connective Tissue Antibodies  >2 publications with SMPD1 and Connective Tissue
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