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SMURF2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6349148

SMURF2 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein interacts with SMAD1, SMAD2 and SMAD7 in order… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SMURF2, Polyclonal Antibody
Full Product Name
SMURF2, CT (SMURF2, E3 ubiquitin-protein ligase SMURF2, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2) (Azide free) (HRP)
Product Gene Name
anti-SMURF2 antibody
Product Synonym Names
SMURF2; E3 ubiquitin-protein ligase SMURF2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein G Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
Predicted: 82 kDa

Observed: 85 kDa
OMIM
605532
3D Structure
ModBase 3D Structure for Q9HAU4
Immunogen
SMURF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 714~743 amino acids from the C-terminal region of human SMURF2.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-SMURF2 antibody

Antibodies; Ubiquitin Ligases
Applications Tested/Suitable for anti-SMURF2 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-SMURF2 antibody
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-SMURF2 antibody

Western blot analysis of anti-SMURF2 Pab in 293 cell line lysate (35ug/lane). SMURF2(arrow) was detected using the purified Pab
Rabbit Anti-SMURF2 Polyclonal Antibody – Cat# MBS6349148

Immunohistochemistry (IHC) of anti-SMURF2 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-SMURF2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6349148

Testing Data of anti-SMURF2 antibody

Hippocampal neurons were fixed at stage 3, stained with anti-Smurf2 (red) and anti-Kinesin-2 (green) antibodies, and analyzed by confocal microscopy. The panels show single confocal planes. (J. Biol. Chem. 2007 Nov 30;282(48):35259-35268)
Rabbit Anti-SMURF2 Polyclonal Antibody, Testing Data – Cat# MBS6349148

Testing Data of anti-SMURF2 antibody

Hippocampal neurons were transfected 2 h after plating with expression vectors for EGFP, EGFP-tagged Par3-4N/2, Par3-PDZ2, Par3-PDZ3, Smurf2-HECT (HECT), Smurf2-HECT-C716A (HECT CA), and shRNA directed against mPar3 (Par3 RNAi), or vectors for the anti-Par3 shRNA and human Myc-Par3 (RNAi + h Par3) (green). Transfected cells were analyzed at 3 d.i.v. by staining with an anti-Smurf2 antibody (red). Axons are marked by arrowheads. The marked growth cones are shown at a higher magnification. Scale bars, 40 and 10 ?. (J. Biol. Chem. 2007 Nov 30;282(48):35259-35268)
Rabbit Anti-SMURF2 Polyclonal Antibody, Testing Data – Cat# MBS6349148

Product References and Citations for anti-SMURF2 antibody

Tajima, Y., et al., J. Biol. Chem. 278(12):10716-10721 (2003). Suzuki, C., et al., J. Biol. Chem. 277(42):39919-39925 (2002). Ebisawa, T., et al., J. Biol. Chem. 276(16):12477-12480 (2001). Zhu, H., et al., Nature 400(6745):687-693 (1999). Lambris, J., et al., J. Immunol. Methods 27(1):55-59 (1979).

Other Notes

Small volumes of anti-SMURF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SMURF2 purchase

MBS6349148 is an antibody targeting SMURF2. Also known as SMURF2, CT (SMURF2, E3 ubiquitin-protein ligase SMURF2, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2) (Azide free) (HRP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SMURF2. It may not necessarily be applicable to this product.

NCBI GI #
12232397
NCBI GeneID
64750
NCBI Accession #
NP_073576
UniProt Primary Accession #
Q9HAU4
UniProt Secondary Accession #
Q52LL1; Q9H260
UniProt Related Accession #
Q9HAU4
GenBank Nucleotide #
NM_022739.3
NCBI Official Full Name
E3 ubiquitin-protein ligase SMURF2
NCBI Official Synonym Full Names
SMAD specific E3 ubiquitin protein ligase 2
NCBI Official Symbol
SMURF2
UniProt Protein Name
E3 ubiquitin-protein ligase SMURF2
UniProt Synonym Protein Names
SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2
Protein Family
E3 ubiquitin-protein ligase
UniProt Gene Name
SMURF2
UniProt Synonym Gene Names
hSMURF2
UniProt Entry Name
SMUF2_HUMAN
NCBI Protein Information
E3 ubiquitin-protein ligase SMURF2; hSMURF2; E3 ubiquitin ligase SMURF2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2

UniProt Comments for SMURF2

SMURF2: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.

Protein type: EC 6.3.2.-; EC 6.3.2.19; Ligase; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 17q22-q23

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol; nucleus; ubiquitin ligase complex; lipid raft

Molecular Function: identical protein binding; protein binding; ubiquitin-protein ligase activity; SMAD binding; transforming growth factor beta receptor binding; ligase activity

Biological Process: BMP signaling pathway; ubiquitin-dependent protein catabolic process; transcription initiation from RNA polymerase II promoter; regulation of transforming growth factor beta receptor signaling pathway; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; protein ubiquitination during ubiquitin-dependent protein catabolic process; gene expression; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; ubiquitin-dependent SMAD protein catabolic process
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Research Articles on SMURF2

1. The SMURF2:UBCH5 complex is critical in maintaining KRAS protein stability.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SMURF2 antibody

 Reference Product  PubMed Publications
 SMAD7 antibody  >60 publications with SMURF2 and SMAD7
 SMAD2 antibody  >52 publications with SMURF2 and SMAD2
 SMURF1 antibody  >48 publications with SMURF2 and SMURF1
 SMAD3 antibody  >38 publications with SMURF2 and SMAD3
 SMAD1 antibody  >11 publications with SMURF2 and SMAD1
 SMAD5 antibody  >6 publications with SMURF2 and SMAD5
 RNF11 antibody  >3 publications with SMURF2 and RNF11
 TGFBR1 antibody  >1 publications with SMURF2 and TGFBR1

Pathways associated with anti-SMURF2 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Asymmetric Localization Of PCP Proteins Pathway antibodies  Asymmetric Localization Of PCP Proteins Pathway Diagram
 BMP Receptor Signaling Pathway antibodies  BMP Receptor Signaling Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downregulation Of SMAD2/3:SMAD4 Transcriptional Activity Pathway antibodies  Downregulation Of SMAD2/3:SMAD4 Transcriptional Activity Pathway Diagram
 Downregulation Of TGF-beta Receptor Signaling Pathway antibodies  Downregulation Of TGF-beta Receptor Signaling Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram

Diseases associated with anti-SMURF2 antibody

 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >22 publications with SMURF2 and Fibrosis
 Kidney Diseases Antibodies  >15 publications with SMURF2 and Kidney Diseases
 Breast Neoplasms Antibodies  >13 publications with SMURF2 and Breast Neoplasms
 Disease Models, Animal Antibodies  >11 publications with SMURF2 and Disease Models, Animal
 Skin Diseases Antibodies  >10 publications with SMURF2 and Skin Diseases
 Necrosis Antibodies  >9 publications with SMURF2 and Necrosis
 Inflammation Antibodies  >7 publications with SMURF2 and Inflammation
 Nervous System Diseases Antibodies  >5 publications with SMURF2 and Nervous System Diseases
 Proteinuria Antibodies  >4 publications with SMURF2 and Proteinuria
 Cell Transformation, Neoplastic Antibodies  >3 publications with SMURF2 and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-SMURF2 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >31 publications with SMURF2 and Bone
 Kidney Antibodies  >20 publications with SMURF2 and Kidney
 Lung Antibodies  >10 publications with SMURF2 and Lung
 Muscle Antibodies  >9 publications with SMURF2 and Muscle
 Blood Antibodies  >9 publications with SMURF2 and Blood
 Connective Tissue Antibodies  >6 publications with SMURF2 and Connective Tissue
 Embryonic Tissue Antibodies  >6 publications with SMURF2 and Embryonic Tissue
 Heart Antibodies  >5 publications with SMURF2 and Heart
 Bone Marrow Antibodies  >4 publications with SMURF2 and Bone Marrow
 Nerve Antibodies  >4 publications with SMURF2 and Nerve
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