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SOX4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6350080

SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The protein may act as a transcriptional regulator after forming a protein complex… More details

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Product Overview

Online Datasheet in PDF
Product Name
SOX4, Polyclonal Antibody
Full Product Name
SOX4, NT (SOX4, Transcription factor SOX-4) (MaxLight 490)
Product Gene Name
anti-SOX4 antibody
Product Synonym Names
SOX4; Transcription factor SOX-4
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
Predicted: 52 kDa

Observed: 520 kDa
OMIM
184430
3D Structure
ModBase 3D Structure for Q06945
Immunogen
SOX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93~122 amino acids from the N-terminal region of human SOX4.
Conjugate
MaxLight490
Note
Preservative Free

Product Categories/Family for anti-SOX4 antibody

Antibodies; Transcription Factors
Applications Tested/Suitable for anti-SOX4 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-SOX4 antibody
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:50-100
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-SOX4 antibody

Western blot analysis of SOX4 antibody (N-term) in A2058 cell line lysates (35ug/lane). SOX4 (arrow) was detected using the purified Pab.
Rabbit Anti-SOX4 Polyclonal Antibody – Cat# MBS6350080

Immunohistochemistry (IHC) of anti-SOX4 antibody

Formalin-fixed and paraffin-embedded human brain reacted with SOX4 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-SOX4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6350080

Flow Cytometry (FC/FACS) of anti-SOX4 antibody

SOX4 Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-SOX4 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6350080

Immunofluorescence (IF) of anti-SOX4 antibody

Fluorescent confocal image of Hela cell stained with SOX4 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX4 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SOX4 immunoreactivity is localized to Mitochondria significantly and Nucleus weakly.
Rabbit Anti-SOX4 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6350080

Product References and Citations for anti-SOX4 antibody

Farr C.J., Easty D.J.Mamm. Genome 4:577-584(1993) Pan X., Li H., Zhang P.Biochem. Biophys. Res. Commun. 344:727-734(2006).

Other Notes

Small volumes of anti-SOX4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SOX4 purchase

MBS6350080 is an antibody targeting SOX4. Also known as SOX4, NT (SOX4, Transcription factor SOX-4) (MaxLight 490). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SOX4. It may not necessarily be applicable to this product.

NCBI GI #
4507163
NCBI GeneID
6659
NCBI Accession #
NP_003098
UniProt Primary Accession #
Q06945
UniProt Related Accession #
Q06945
GenBank Nucleotide #
NM_003107.2
NCBI Official Full Name
transcription factor SOX-4
NCBI Official Synonym Full Names
SRY (sex determining region Y)-box 4
NCBI Official Symbol
SOX4
NCBI Official Synonym Symbols
EVI16
UniProt Protein Name
Transcription factor SOX-4
Protein Family
Transcription factor
UniProt Gene Name
SOX4
UniProt Entry Name
SOX4_HUMAN
NCBI Protein Information
transcription factor SOX-4; SRY-related HMG-box gene 4; ecotropic viral integration site 16

NCBI Summary for SOX4

This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein. [provided by RefSeq, Jul 2008]
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UniProt Comments for SOX4

SOX4: Transcriptional activator that binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3' motif.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus

Molecular Function: protein binding; protein heterodimerization activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; negative regulation of protein export from nucleus; positive regulation of translation; somatic stem cell maintenance; positive regulation of apoptosis; heart development; positive regulation of transcription, DNA-dependent; regulation of protein stability; Wnt receptor signaling pathway through beta-catenin; glucose homeostasis; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; glial cell proliferation; negative regulation of cell proliferation; sympathetic nervous system development; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of Wnt receptor signaling pathway; skeletal development; T cell differentiation; glial cell development; pro-B cell differentiation; protein stabilization; positive regulation of insulin secretion; DNA damage response, detection of DNA damage; spinal cord motor neuron differentiation; endocrine pancreas development; limb bud formation; spinal cord development; negative regulation of protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter; neural tube formation
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Research Articles on SOX4

1. SOX4 is associated with poor prognosis in prostate cancer and promotes epithelial-mesenchymal transition in vitro.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SOX4 antibody

 Reference Product  PubMed Publications
 MEF2C antibody  >3 publications with SOX4 and MEF2C
 JUP antibody  >2 publications with SOX4 and JUP
 EGFR antibody  >2 publications with SOX4 and EGFR
 DICER1 antibody  >2 publications with SOX4 and DICER1
 SDCBP antibody  >2 publications with SOX4 and SDCBP
 TP53 antibody  >2 publications with SOX4 and TP53
 CREBBP antibody  >1 publications with SOX4 and CREBBP
 TBX3 antibody  >1 publications with SOX4 and TBX3

Pathways associated with anti-SOX4 antibody

 Products by Pathway  Pathway Diagram
 IL-5 Signaling Pathway antibodies  IL-5 Signaling Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram

Diseases associated with anti-SOX4 antibody

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >19 publications with SOX4 and Adenocarcinoma
 Nervous System Diseases Antibodies  >17 publications with SOX4 and Nervous System Diseases
 Breast Neoplasms Antibodies  >15 publications with SOX4 and Breast Neoplasms
 Disease Models, Animal Antibodies  >14 publications with SOX4 and Disease Models, Animal
 Lung Neoplasms Antibodies  >13 publications with SOX4 and Lung Neoplasms
 Cell Transformation, Neoplastic Antibodies  >12 publications with SOX4 and Cell Transformation, Neoplastic
 Neoplasms, Experimental Antibodies  >9 publications with SOX4 and Neoplasms, Experimental
 Prostatic Neoplasms Antibodies  >9 publications with SOX4 and Prostatic Neoplasms
 Liver Diseases Antibodies  >8 publications with SOX4 and Liver Diseases
 Liver Neoplasms Antibodies  >7 publications with SOX4 and Liver Neoplasms

Organs/Tissues associated with anti-SOX4 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >34 publications with SOX4 and Blood
 Brain Antibodies  >30 publications with SOX4 and Brain
 Embryonic Tissue Antibodies  >28 publications with SOX4 and Embryonic Tissue
 Bone Antibodies  >26 publications with SOX4 and Bone
 Lung Antibodies  >25 publications with SOX4 and Lung
 Heart Antibodies  >19 publications with SOX4 and Heart
 Liver Antibodies  >13 publications with SOX4 and Liver
 Bone Marrow Antibodies  >12 publications with SOX4 and Bone Marrow
 Nerve Antibodies  >12 publications with SOX4 and Nerve
 Prostate Antibodies  >11 publications with SOX4 and Prostate
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