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SPAG1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6350261

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved identification and treatment of immunologic infertility, as well as identification of proteins for… More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SPAG1, Polyclonal Antibody
Full Product Name
SPAG1, NT (SPAG1, Sperm-associated antigen 1, HSD-3.8, Infertility-related sperm protein Spag-1) (AP)
Product Gene Name
anti-SPAG1 antibody
Product Synonym Names
SPAG1; Sperm-associated antigen 1; HSD-3.8; Infertility-related sperm protein Spag-1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
48,031 Da
OMIM
gene 615505
3D Structure
ModBase 3D Structure for Q07617
Immunogen
SPAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-159 amino acids from the N-terminal region of human SPAG1.
Conjugate
AP
Note
Preservative Free

Product Categories/Family for anti-SPAG1 antibody

Antibodies; Spermatogenesis; Testis Proteins
Applications Tested/Suitable for anti-SPAG1 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-SPAG1 antibody
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-SPAG1 antibody

Western Blot analysis using MBS645181 in Jurkat cell line lysates (35ug/lane). This demonstrates the SPAG1 antibody detected the SPAG1 protein (arrow).
Rabbit Anti-SPAG1 Polyclonal Antibody – Cat# MBS6350261

Immunohistochemistry (IHC) of anti-SPAG1 antibody

Immunohistochemistry analysis using MBS645181 in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Rabbit Anti-SPAG1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6350261

Product References and Citations for anti-SPAG1 antibody

Lin, W., et al. Mol. Hum. Reprod. 7(9):811-818(2001). Diekman, A.B., et al. Am. J. Reprod. Immunol. 37(1):111-117(1997). Zhang, M.L., et al. Chin. Med. J. 105(12):998-1003(1992).

Other Notes

Small volumes of anti-SPAG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPAG1 purchase

MBS6350261 is an antibody targeting SPAG1. Also known as SPAG1, NT (SPAG1, Sperm-associated antigen 1, HSD-3.8, Infertility-related sperm protein Spag-1) (AP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SPAG1. It may not necessarily be applicable to this product.

NCBI GI #
27262636
NCBI GeneID
6674
NCBI Accession #
NP_003105.2
UniProt Primary Accession #
Q07617
UniProt Secondary Accession #
Q7Z5G1; A6NP70; B3KQ58; G3XAM3
UniProt Related Accession #
Q07617
GenBank Nucleotide #
NM_003114.4
NCBI Official Full Name
sperm-associated antigen 1
NCBI Official Synonym Full Names
sperm associated antigen 1
NCBI Official Symbol
SPAG1
NCBI Official Synonym Symbols
SP75; TPIS; CT140; CILD28; HSD-3.8; HEL-S-268
UniProt Protein Name
Sperm-associated antigen 1
UniProt Synonym Protein Names
HSD-3.8; Infertility-related sperm protein Spag-1
Protein Family
Sperm-associated antigen
UniProt Gene Name
SPAG1
UniProt Entry Name
SPAG1_HUMAN
NCBI Protein Information
sperm-associated antigen 1

NCBI Summary for SPAG1

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. Furthermore, immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombinant polypeptide binds GTP and exhibits GTPase activity. Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein. [provided by RefSeq, Jul 2008]
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UniProt Comments for SPAG1

SPAG1: Plays a role in fertilization. Binds GTP and has GTPase activity.

Protein type: Cancer Testis Antigen (CTA); Microtubule-binding

Chromosomal Location of Human Ortholog: 8q22.2

Cellular Component: nucleoplasm; microtubule cytoskeleton; cytoplasm

Molecular Function: GTP binding; hydrolase activity

Biological Process: metabolic process; single fertilization

Disease: Ciliary Dyskinesia, Primary, 28
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Research Articles on SPAG1

1. SPAG1 probably plays a role in the cytoplasmic assembly.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-SPAG1 antibody

 Disease Name  Pubmed Publications
 Infertility, Female Antibodies  >2 publications with SPAG1 and Infertility, Female
 Congenital Abnormalities Antibodies  >1 publications with SPAG1 and Congenital Abnormalities
 Lung Neoplasms Antibodies  >1 publications with SPAG1 and Lung Neoplasms
 Breast Neoplasms Antibodies  >1 publications with SPAG1 and Breast Neoplasms

Organs/Tissues associated with anti-SPAG1 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >7 publications with SPAG1 and Testis
 Skin Antibodies  >1 publications with SPAG1 and Skin
 Embryonic Tissue Antibodies  >1 publications with SPAG1 and Embryonic Tissue
 Lung Antibodies  >1 publications with SPAG1 and Lung
 Kidney Antibodies  >1 publications with SPAG1 and Kidney
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