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SPAG1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS7127705

MBS7127705 is an antibody targeting SPAG1. Also known as SPAG1 Antibody. Reactive in Human. Host: Rabbit. More details

$230 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SPAG1, Polyclonal Antibody
Full Product Name
SPAG1 Antibody
Product Gene Name
anti-SPAG1 antibody
Product Synonym Names
Sperm associated antigen 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid; In Phosphate Buffered Saline, pH 7.4, 0.05% NaN3, and 40% Glycerol
Purity/Purification
Antigen Affinity Purification
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
Molecular Weight
926
OMIM
gene 615505
3D Structure
ModBase 3D Structure for Q07617
Immunogen
Synthetic peptide of human SPAG1
Immunogen Species
Human
Conjugate
Non-conjugated

Product Categories/Family for anti-SPAG1 antibody

Developmental Biology
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-SPAG1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-SPAG1 antibody
ELISA: 1:1000-1:5000
WB: 1:500-1:2000
IHC: 1:25-1:100

Immunohistochemistry (IHC)-Paraffin of anti-SPAG1 antibody

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using MBS7127705(SPAG1 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)
Rabbit Anti-SPAG1 Polyclonal Antibody – Cat# MBS7127705

Immunohistochemistry (IHC)-Paraffin of anti-SPAG1 antibody

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using MBS7127705(SPAG1 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200)
Rabbit Anti-SPAG1 Polyclonal Antibody, Immunohistochemistry (IHC)-Paraffin – Cat# MBS7127705

SDS-Page of anti-SPAG1 antibody

Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human colon tissue, Primary antibody: MBS7127705(SPAG1 Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
Rabbit Anti-SPAG1 Polyclonal Antibody, SDS-Page – Cat# MBS7127705

Other Notes

Small volumes of anti-SPAG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SPAG1. It may not necessarily be applicable to this product.

NCBI GI #
223634718
NCBI GeneID
6674
NCBI Accession #
Q07617.3
UniProt Primary Accession #
Q07617
UniProt Secondary Accession #
Q7Z5G1; A6NP70; B3KQ58; G3XAM3
UniProt Related Accession #
Q07617
NCBI Official Full Name
Sperm-associated antigen 1
NCBI Official Synonym Full Names
sperm associated antigen 1
NCBI Official Symbol
SPAG1
NCBI Official Synonym Symbols
SP75; TPIS; CT140; CILD28; HSD-3.8
UniProt Protein Name
Sperm-associated antigen 1
UniProt Synonym Protein Names
HSD-3.8; Infertility-related sperm protein Spag-1
Protein Family
Sperm-associated antigen
UniProt Gene Name
SPAG1
UniProt Entry Name
SPAG1_HUMAN
NCBI Protein Information
sperm-associated antigen 1; infertility-related sperm protein Spag-1; tetratricopeptide repeat-containing protein; TPR-containing protein involved in spermatogenesis

NCBI Summary for SPAG1

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. Furthermore, immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombinant polypeptide binds GTP and exhibits GTPase activity. Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein. [provided by RefSeq, Jul 2008]
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UniProt Comments for SPAG1

SPAG1: Plays a role in fertilization. Binds GTP and has GTPase activity.

Protein type: Cancer Testis Antigen (CTA); Microtubule-binding

Chromosomal Location of Human Ortholog: 8q22.2

Cellular Component: nucleoplasm; microtubule cytoskeleton; cytoplasm

Molecular Function: GTP binding; hydrolase activity

Biological Process: metabolic process; single fertilization

Disease: Ciliary Dyskinesia, Primary, 28
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Research Articles on SPAG1

1. SPAG1 probably plays a role in the cytoplasmic assembly.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-SPAG1 antibody

 Disease Name  Pubmed Publications
 Infertility, Female Antibodies  >2 publications with SPAG1 and Infertility, Female
 Heart Diseases Antibodies  >1 publications with SPAG1 and Heart Diseases
 Genital Diseases, Male Antibodies  >1 publications with SPAG1 and Genital Diseases, Male
 Cardiovascular Diseases Antibodies  >1 publications with SPAG1 and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >1 publications with SPAG1 and Breast Neoplasms
 Congenital Abnormalities Antibodies  >1 publications with SPAG1 and Congenital Abnormalities

Organs/Tissues associated with anti-SPAG1 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >7 publications with SPAG1 and Testis
 Skin Antibodies  >1 publications with SPAG1 and Skin
 Embryonic Tissue Antibodies  >1 publications with SPAG1 and Embryonic Tissue
 Lung Antibodies  >1 publications with SPAG1 and Lung
 Kidney Antibodies  >1 publications with SPAG1 and Kidney
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