Rabbit Anti-SPON2 Polyclonal Antibody – Cat# MBS1758818

Figure 1. Western blot analysis of SPON2 using anti-SPON2 antibody (MBS1758818).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human LNCAP whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPON2 antigen affinity purified polyclonal antibody (#MBS1758818) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for SPON2 at approximately 39 kDa. The expected band size for SPON2 is at 36 kDa.

SPON2 Antibody – Rabbit Polyclonal

Name: SPON2 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1758818
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758818

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SPON2, Polyclonal Antibody

Full Product Name

Anti-SPON2 Antibody Picoband

Product Gene Name

anti-SPON2 antibody

Product Synonym Names

BAG family molecular chaperone regulator 5; BAG family molecular chaperone regulator 5; BAG family molecular chaperone regulator 5; BAG-5; Bcl-2-associated athanogene 5; BAG5; KIAA0873

Clonality

Polyclonal

Isotype

Rabbit IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purity/Purification

Immunogen affinity purified.

Specificity

Expressed in all tissues examined with lower levels in brain and testis.

Preparation and Storage

At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.

Molecular Weight

35,846 Da

Chromosome Location

Chromosome: 4; NC_000004.11 (1160720..1202750, complement). Location: 4p16.3

OMIM

605918

3D Structure

ModBase 3D Structure for Q9BUD6

Immunogen

E coli-derived human SPON2 recombinant protein (Position: Q53-V331).

Subcellular Localization

Perinuclear region

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.

Cross Reactivity

No cross-reactivity with other proteins.

Protein Function

Inhibits both auto-ubiquitination of PARK2 and ubiquitination of target proteins by PARK2 (By similarity). May function as a nucleotide exchange factor for HSP/HSP70, promoting ADP release, and activating Hsp70-mediated refolding.

Product Categories/Family for anti-SPON2 antibody

Primary Antibodies; Interleukins

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-SPON2 antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-SPON2 antibody

WB: 0.25-0.5ug/ml, Human
ELISA: 0.1-0.5ug/ml

Product References and Citations for anti-SPON2 antibody

1. He, Y.-W., Li, H., Zhang, J., Hsu, C.-L., Lin, E., Zhang, N., Guo, J., Forbush, K. A., Bevan, M. J. The extracellular matrix protein mindin is a pattern-recognition molecule for microbial pathogens. Nature Immun. 5: 88-97, 2004. 2. Manda, R., Kohno, T., Matsuno, Y., Takenoshita, S., Kuwano, H., Yokota, J. Identification of genes (SPON2 and C20orf2) differentially expressed between cancerous and noncancerous lung cells by mRNA differential display. Genomics 61: 5-14, 1999.

Other Notes

Small volumes of anti-SPON2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPON2 purchase

MBS1758818 is an antibody targeting SPON2. Also known as Anti-SPON2 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SPON2. It may not necessarily be applicable to this product.

NCBI GI #

190341087

NCBI GeneID

10417

NCBI Accession #

NP_001121797.1

UniProt Primary Accession #

Q9BUD6

UniProt Secondary Accession #

Q4W5N4; Q9ULW1

UniProt Related Accession #

Q9BUD6

GenBank Nucleotide #

NM_001128325.2

NCBI Official Full Name

spondin-2

NCBI Official Synonym Full Names

spondin 2, extracellular matrix protein

NCBI Official Symbol

SPON2

NCBI Official Synonym Symbols

DIL1; DIL-1; MINDIN; M-SPONDIN

UniProt Protein Name

Spondin-2

UniProt Synonym Protein Names

Differentially expressed in cancerous and non-cancerous lung cells 1; DIL-1; Mindin

Protein Family

Spondin

UniProt Gene Name

SPON2

UniProt Synonym Gene Names

DIL1; DIL-1

UniProt Entry Name

SPON2_HUMAN

NCBI Protein Information

spondin-2; differentially expressed in cancerous and non-cancerous lung cells 1

UniProt Comments for SPON2

SPON2: Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern- recognition molecule in the ECM for microbial pathogens. Binds bacterial lipopolysaccharide (LPS).

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: extracellular space; proteinaceous extracellular matrix

Molecular Function: lipopolysaccharide binding; metal ion binding; antigen binding

Biological Process: induction of bacterial agglutination; mast cell mediated immunity; axon guidance; innate immune response; positive regulation of interleukin-6 production; opsonization; cell adhesion; positive regulation of tumor necrosis factor production; defense response to fungus; defense response to virus

Research Articles on SPON2

1. Using tissue microarray by immunohistostaining, we found SPON2 to be over-expressed in prostate cancer (PCa). SPON2 staining was more intense in Gleason score sum 7-8 and in PCa patients with metastasis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-SPON2 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>8 publications with SPON2 and Neoplasms
Carcinoma Antibodies	>3 publications with SPON2 and Carcinoma
Ovarian Neoplasms Antibodies	>2 publications with SPON2 and Ovarian Neoplasms
Necrosis Antibodies	>2 publications with SPON2 and Necrosis
Fibrosis Antibodies	>1 publications with SPON2 and Fibrosis
Lung Neoplasms Antibodies	>1 publications with SPON2 and Lung Neoplasms
Drug Toxicity Antibodies	>1 publications with SPON2 and Drug Toxicity
Liver Neoplasms Antibodies	>1 publications with SPON2 and Liver Neoplasms
Kidney Diseases Antibodies	>1 publications with SPON2 and Kidney Diseases
Carcinoma, Hepatocellular Antibodies	>1 publications with SPON2 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-SPON2 antibody

Organ/Tissue Name	Pubmed Publications
Prostate Antibodies	>5 publications with SPON2 and Prostate
Ovary Antibodies	>1 publications with SPON2 and Ovary