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SRC3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3900950

Steroid receptor coactivator 3 (SRC3) belongs to the SRC family of proteins that interacts with nuclear receptors to facilitate transcription. The 3 homologous members of this family are SRC-1 (NCoA-1), SRC-2 (GRIP1, TIF2, or NcoA-2), and SRC-3 (p/CIP, RAC3,… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SRC3, Polyclonal Antibody
Full Product Name
Rabbit anti-SRC3 Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
NCOA3
Product Synonym Names
TRAM-1; RAC3; RAC-3; receptor-associated coactivator 3; SRC3; SRC-3; steroid receptor coactivator protein 3; thyroid hormone receptor activator molecule 1; pCIP; TNRC16; class E basic helix-loop-helix protein 42; TNRC14; nuclear receptor coactivator 3; NCoA-3; CTG26; CBP-interacting protein; CAGH16; bHLHe42; amplified in breast cancer 1 protein; AIB-1; AIB1; ACTR; KAT13B; Nuclear receptor coactivator 3; nuclear receptor coactivator 3
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
154,892 Da
OMIM
601937
3D Structure
ModBase 3D Structure for Q9Y6Q9
Immunogen
Between 575 and 625
Conjugation
Unconjugated

Product Categories/Family for anti-SRC3 antibody

Applications Tested/Suitable for anti-SRC3 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-SRC3 antibody
WB: 1:1000-1:10,000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-SRC3 antibody

Detection of human SRC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SRC3 antibody MBS3900950 (lot MBS3900950-2) used for IP at 6 ug per reaction. SRC3 was also immunoprecipitated by a previous lot of this antibody (lot MBS3900950-1). For blotting immunoprecipitated SRC3, MBS3900950 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-SRC3 Polyclonal Antibody – Cat# MBS3900950

Western Blot (WB) of anti-SRC3 antibody

Detection of human SRC3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SRC3 antibody MBS3900950 (lot MBS3900950-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-SRC3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3900950

Other Notes

Small volumes of anti-SRC3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SRC3 purchase

MBS3900950 is an antibody targeting SRC3. Also known as Rabbit anti-SRC3 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SRC3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens nuclear receptor coactivator 3 (NCOA3), transcript variant 1, mRNA
NCBI Official Synonym Full Names
nuclear receptor coactivator 3
NCBI Official Symbol
NCOA3
NCBI Official Synonym Symbols
ACTR; AIB1; RAC3; SRC3; pCIP; AIB-1; CTG26; SRC-3; CAGH16; KAT13B; TNRC14; TNRC16; TRAM-1; bHLHe42
UniProt Protein Name
Nuclear receptor coactivator 3
UniProt Synonym Protein Names
ACTR; Amplified in breast cancer 1 protein; AIB-1; CBP-interacting protein; pCIP; Class E basic helix-loop-helix protein 42; bHLHe42; Receptor-associated coactivator 3; RAC-3; Steroid receptor coactivator protein 3; SRC-3; Thyroid hormone receptor activator molecule 1
UniProt Gene Name
NCOA3
UniProt Synonym Gene Names
AIB1; BHLHE42; RAC3; TRAM1; NCoA-3; AIB-1; pCIP; bHLHe42; RAC-3; SRC-3; TRAM-1
UniProt Entry Name
NCOA3_HUMAN
NCBI Protein Information
nuclear receptor coactivator 3; CBP-interacting protein; amplified in breast cancer 1 protein; class E basic helix-loop-helix protein 42; receptor-associated coactivator 3; steroid receptor coactivator protein 3; thyroid hormone receptor activator molecul

NCBI Summary for SRC3

The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein. [provided by RefSeq, Mar 2010]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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