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TCF7L1 Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177921

Description: Rabbit IgG polyclonal antibody for Transcription factor 7-like 1(TCF7L1) detection. Tested with WB, IHC-P in Human.Background: Transcription factor 7-like 1 (T-cell specific, HMG-box), also known as TCF7L1, is a human gene. This gene encodes a… More details

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Product Overview

Online Datasheet in PDF
Product Name
TCF7L1, Polyclonal Antibody
Full Product Name
Anti-TCF7L1 Antibody
Product Gene Name
anti-TCF7L1 antibody
Product Synonym Names
Transcription factor 7-like 1; bHLHb21; HMG box transcription factor 3; HMG box transcription factor; OTTMUSP00000023419; T cell factor 3; TCF 3; TCF-3; TCF3; Tcf7l1; TF7L1_HUMAN; Transcription factor 7 like 1 (T-cell specific, HMG-box); Transcription factor 7 like 1; Transcription factor 7-like 1; transcription factor 7 like 1
Clonality
Polyclonal
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Specificity
No cross reactivity with other proteins.
Preparation and Storage
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
OMIM
604652
3D Structure
ModBase 3D Structure for Q9HCS4
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TCF7L1 (561-588aa SFPATLHAHQALPVLQAQPLSLVTKSAH), different from the related mouse sequence by one amino acid.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TCF7L1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-TCF7L1 antibody
Western Blot

Concentration :0.1-0.5 ug/ml
Tested Species: Hu


Immunohistochemistry (Paraffin-embedded Section) :

Concentration: 0.5-1ug/ml
Tested Species: Hu
Antigen Retrieval: By Heat


Tested Species: In house tested species with positive results.

By Heat: Boiling the paraffin sections in 10 mm citrate buffer, pH 6.0 for 20 mins is required for the staining of formalin/paraffin sections.

Other applications have not been tested. .

Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-TCF7L1 antibody

Western blot analysis of TCF7L1 expression in COLO320 whole cell lysates (lane 1). TCF7L1 at 63KD was detected using rabbit anti- TCF7L1 Antigen Affinity purified polyclonal antibody at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.
Anti-TCF7L1 Polyclonal Antibody – Cat# MBS177921

Immunohistochemistry (IHC) of anti-TCF7L1 antibody

TCF7L1 was detected in paraffin-embedded sections of human oesophagus squama cancer tissues using rabbit anti- TCF7L1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Anti-TCF7L1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177921

Product References and Citations for anti-TCF7L1 antibody

1. "Entrez Gene: transcription factor 7-like 1 (T-cell specific, HMG-box)". 2. Castrop, J., van Norren, K., Clevers, H. A gene family of HMG-box transcription factors with homology to TCF-1. Nucleic Acids Res. 20: 611 only, 1992. 3. Sagara, N., Katoh, M. Mitomycin C resistance induced by TCF-3 overexpression in gastric cancer cell line MKN28 is associated with DT-diaphorase down-regulation. Cancer Res. 60: 5959-5962, 2000.

Other Notes

Small volumes of anti-TCF7L1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TCF7L1 purchase

MBS177921 is an antibody targeting TCF7L1. Reactive in Human.

Additional Details

NCBI/Uniprot data below describe general gene information for TCF7L1. It may not necessarily be applicable to this product.

NCBI GI #
13786123
NCBI GeneID
83439
NCBI Accession #
NP_112573.1
UniProt Primary Accession #
Q9HCS4
UniProt Secondary Accession #
Q53R97; Q6PD70; Q9NP00
UniProt Related Accession #
Q9HCS4
GenBank Nucleotide #
NM_031283.2
NCBI Official Full Name
transcription factor 7-like 1
NCBI Official Synonym Full Names
transcription factor 7 like 1
NCBI Official Symbol
TCF7L1
NCBI Official Synonym Symbols
TCF3; TCF-3
UniProt Protein Name
Transcription factor 7-like 1
UniProt Synonym Protein Names
HMG box transcription factor 3; TCF-3
Protein Family
Transcription factor
UniProt Gene Name
TCF7L1
UniProt Synonym Gene Names
TCF3; TCF-3
UniProt Entry Name
TF7L1_HUMAN
NCBI Protein Information
transcription factor 7-like 1

NCBI Summary for TCF7L1

This gene encodes a member of the T cell factor/lymphoid enhancer factor family of transcription factors. These transcription factors are activated by beta catenin, mediate the Wnt signaling pathway and are antagonized by the transforming growth factor beta signaling pathway. The encoded protein contains a high mobility group-box DNA binding domain and participates in the regulation of cell cycle genes and cellular senescence. [provided by RefSeq, Nov 2010]
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UniProt Comments for TCF7L1

TCF3: Participates in the Wnt signaling pathway. Binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. Necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis. Down-regulates NQO1, leading to increased mitomycin c resistance. Belongs to the TCF/LEF family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: nucleoplasm; nucleus; transcription factor complex

Molecular Function: beta-catenin binding; DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: establishment and/or maintenance of chromatin architecture; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; regulation of Wnt receptor signaling pathway; transcription, DNA-dependent; Wnt receptor signaling pathway through beta-catenin
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Research Articles on TCF7L1

1. Data indicate that median methylation levels of BCAN, HOXD1, KCTD8, KLF11, NXPH1, POU4F1, SIM1, and TCF7L1 were >/=30% higher than in normal samples, representing potential biomarkers for tumor diagnosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TCF7L1 antibody

 Reference Product  PubMed Publications
 CTNNB1 antibody  >46 publications with TCF7L1 and CTNNB1
 EP300 antibody  >8 publications with TCF7L1 and EP300
 CREBBP antibody  >3 publications with TCF7L1 and CREBBP
 CCND1 antibody  >2 publications with TCF7L1 and CCND1
 AXIN2 antibody  >2 publications with TCF7L1 and AXIN2
 TLE3 antibody  >1 publications with TCF7L1 and TLE3
 NLK antibody  >1 publications with TCF7L1 and NLK
 SMAD4 antibody  >1 publications with TCF7L1 and SMAD4

Pathways associated with anti-TCF7L1 antibody

 Products by Pathway  Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Beta-catenin Independent WNT Signaling Pathway antibodies  Beta-catenin Independent WNT Signaling Pathway Diagram

Diseases associated with anti-TCF7L1 antibody

 Disease Name  Pubmed Publications
 Cell Transformation, Neoplastic Antibodies  >14 publications with TCF7L1 and Cell Transformation, Neoplastic
 Pancreatic Neoplasms Antibodies  >6 publications with TCF7L1 and Pancreatic Neoplasms
 Disease Models, Animal Antibodies  >5 publications with TCF7L1 and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >5 publications with TCF7L1 and Neoplasms, Experimental
 Lung Diseases Antibodies  >4 publications with TCF7L1 and Lung Diseases
 Breast Neoplasms Antibodies  >4 publications with TCF7L1 and Breast Neoplasms
 Lung Neoplasms Antibodies  >3 publications with TCF7L1 and Lung Neoplasms
 Necrosis Antibodies  >3 publications with TCF7L1 and Necrosis
 Atrophy Antibodies  >2 publications with TCF7L1 and Atrophy
 Hypertrophy Antibodies  >1 publications with TCF7L1 and Hypertrophy

Organs/Tissues associated with anti-TCF7L1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >109 publications with TCF7L1 and Blood
 Muscle Antibodies  >52 publications with TCF7L1 and Muscle
 Bone Antibodies  >28 publications with TCF7L1 and Bone
 Embryonic Tissue Antibodies  >27 publications with TCF7L1 and Embryonic Tissue
 Pancreas Antibodies  >21 publications with TCF7L1 and Pancreas
 Bone Marrow Antibodies  >18 publications with TCF7L1 and Bone Marrow
 Brain Antibodies  >14 publications with TCF7L1 and Brain
 Heart Antibodies  >10 publications with TCF7L1 and Heart
 Intestine Antibodies  >7 publications with TCF7L1 and Intestine
 Skin Antibodies  >7 publications with TCF7L1 and Skin
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