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TKT Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8103055

Transketolase (TKT) is a thiamine pyrophosphate (vitamin B1)-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway by feeding excess sugar phosphates into the main carbohydrate metabolic pathways to generate biosynthetic… More details

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Product Overview

Online Datasheet in PDF
Product Name
TKT, Polyclonal Antibody
Full Product Name
Anti-TKT Antibody, Rabbit Polyclonal
Product Gene Name
anti-TKT antibody
Product Synonym Names
TKT Antibody, Rabbit PAb, Antigen Affinity Purified; transketolase; Anti-HEL-S-48 Antibody; Anti-HEL107 Antibody; Anti-TK Antibody; Anti-TKT1 Antibody
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid; 0.2um filtered solution in PBS
Purity/Purification
Protein A & Antigen Affinity
Specificity
Human TKT
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Molecular Weight
68,814 Da
OMIM
606781
3D Structure
ModBase 3D Structure for P29401
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human TKT
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human TKT, and purified by antigen affinity chromatography.
Applications Tested/Suitable for anti-TKT antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-TKT antibody
WB: 1:500-1:2000
ICC/IF: 1:300-1:10,000

Western Blot (WB) of anti-TKT antibody

Anti-TKT rabbit polyclonal antibody at 1:500 dilution
Lane A: HepG2 Whole Cell Lysate
Lane B: HeLa Whole Cell Lysate
Lysates/proteins at 30 ug per lane.
Secondary
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:68 kDa
Observed band size:68 kDa
(We are unsure as to the identity of these extra bands.)
Rabbit Anti-TKT Polyclonal Antibody – Cat# MBS8103055

Immunofluorescence (IF) of anti-TKT antibody

Immunofluorescence staining of TKT in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TKT polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.
Rabbit Anti-TKT Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS8103055

Product References and Citations for anti-TKT antibody

Yang H, et al. (2016) Microrna-497 regulates cisplatin chemosensitivity of cervical cancer by targeting transketolase. Am J Cancer Res 6 (11): 2690-2699.Riyapa D, et al. (2019) Transketolase and vitamin b1 influence on ros-dependent neutrophil extracellular traps (nets) formation. PLoS One 14 (8): e0221016.Markert B, et al. (2014) Characterization of two transketolases encoded on the chromosome and the plasmid pbm19 of the facultative ribulose monophosphate cycle methylotroph bacillus methanolicus. BMC Microbiol 14 7.Li M, et al. (2019) Transketolase deficiency protects the liver from DNA damage by increasing levels of ribose 5-phosphate and nucleotides. Cancer Res 79 (14): 3689-3701.

Other Notes

Small volumes of anti-TKT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TKT purchase

MBS8103055 is an antibody targeting TKT. Also known as Anti-TKT Antibody, Rabbit Polyclonal. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TKT. It may not necessarily be applicable to this product.

NCBI GI #
4507521
NCBI GeneID
7086
NCBI Accession #
NP_001055.1
UniProt Primary Accession #
P29401
UniProt Secondary Accession #
Q8TBA3; Q96HH3; A8K089; B4DE31; E7EPA7
UniProt Related Accession #
P29401
GenBank Nucleotide #
NM_001064.3
NCBI Official Full Name
transketolase isoform 1
NCBI Official Synonym Full Names
transketolase
NCBI Official Symbol
TKT
NCBI Official Synonym Symbols
TK; TKT1; HEL107
UniProt Protein Name
Transketolase
Protein Family
Transketolase
UniProt Gene Name
TKT
UniProt Synonym Gene Names
TK
UniProt Entry Name
TKT_HUMAN
NCBI Protein Information
transketolase; epididymis luminal protein 107

NCBI Summary for TKT

This gene encodes a thiamine-dependent enzyme which plays a role in the channeling of excess sugar phosphates to glycolysis in the pentose phosphate pathway. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012]

UniProt Comments for TKT

TKT: Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate. Homodimer. Belongs to the transketolase family.

Protein type: Transferase; EC 2.2.1.1; Carbohydrate Metabolism - pentose phosphate pathway

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: nucleoplasm; peroxisome; cytosol; nucleus; vesicle

Molecular Function: transketolase activity; protein homodimerization activity; metal ion binding; cofactor binding

Biological Process: pentose-phosphate shunt; regulation of growth; carbohydrate metabolic process; energy reserve metabolic process; glyceraldehyde-3-phosphate biosynthetic process; pentose-phosphate shunt, non-oxidative branch; pathogenesis; xylulose biosynthetic process

Disease: Wernicke-korsakoff Syndrome
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Research Articles on TKT

1. Akt1 phosphorylates TKT on Thr382, markedly enhancing enzyme activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TKT antibody

 Reference Product  PubMed Publications
 PKM2 antibody  >3 publications with TKT and PKM2
 ALDOA antibody  >3 publications with TKT and ALDOA
 RPE antibody  >3 publications with TKT and RPE
 RBKS antibody  >1 publications with TKT and RBKS
 UBC antibody  >1 publications with TKT and UBC
 FBP1 antibody  >1 publications with TKT and FBP1
 RPIA antibody  >1 publications with TKT and RPIA
 TALDO1 antibody  >1 publications with TKT and TALDO1

Pathways associated with anti-TKT antibody

 Products by Pathway  Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Glycogen Storage Diseases Pathway antibodies  Glycogen Storage Diseases Pathway Diagram
 Insulin Effects Increased Synthesis Of Xylulose-5-Phosphate Pathway antibodies  Insulin Effects Increased Synthesis Of Xylulose-5-Phosphate Pathway Diagram
 Integration Of Energy Metabolism Pathway antibodies  Integration Of Energy Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram

Diseases associated with anti-TKT antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >21 publications with TKT and Carcinoma
 Kidney Diseases Antibodies  >9 publications with TKT and Kidney Diseases
 Nervous System Diseases Antibodies  >6 publications with TKT and Nervous System Diseases
 Liver Diseases Antibodies  >6 publications with TKT and Liver Diseases
 Congenital Abnormalities Antibodies  >5 publications with TKT and Congenital Abnormalities
 Inflammation Antibodies  >4 publications with TKT and Inflammation
 Liver Neoplasms Antibodies  >4 publications with TKT and Liver Neoplasms
 Fatty Liver Antibodies  >3 publications with TKT and Fatty Liver
 Hyperplasia Antibodies  >3 publications with TKT and Hyperplasia
 Necrosis Antibodies  >3 publications with TKT and Necrosis

Organs/Tissues associated with anti-TKT antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >17 publications with TKT and Blood
 Eye Antibodies  >14 publications with TKT and Eye
 Liver Antibodies  >13 publications with TKT and Liver
 Kidney Antibodies  >13 publications with TKT and Kidney
 Connective Tissue Antibodies  >9 publications with TKT and Connective Tissue
 Mouth Antibodies  >8 publications with TKT and Mouth
 Bone Antibodies  >7 publications with TKT and Bone
 Brain Antibodies  >7 publications with TKT and Brain
 Lung Antibodies  >5 publications with TKT and Lung
 Heart Antibodies  >5 publications with TKT and Heart
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