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TLE4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9602257

Function: Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be… More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TLE4, Polyclonal Antibody
Full Product Name
TLE4 Antibody
Product Gene Name
anti-TLE4 antibody
Product Synonym Names
B lymphocyte gene 1; E(sp1) homolog Drosophila; E(spI); enhancer of split groucho 4; ESG; ESG4; GRG4; KIAA1261; TIM; TIM1; timeless (Drosophila) homolog; TLE4; Transducin like enhancer protein 4; transducin-like enhancer of split 4
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
TLE4 antibody detects endogenous levels of total TLE4
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
TLE4 peptide (MBS9619711) is used for blocking the activity of TLE4 antibody (MBS9602257)
Molecular Weight
Observed: 83 kDa
Predicted: 84 kDa
3D Structure
ModBase 3D Structure for Q04727
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human TLE4
Subcellular Location
Nuclear
Tissue Specificity
In all tissues examined, mostly in brain, and muscle.
Predicted Cross Reactivity
Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Similarity
Bovine (90%), Horse (90%), Sheep (90%), Rabbit (90%), Dog (100%), Xenopus (90%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-TLE4 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-TLE4 antibody
WB: 1:1000
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-TLE4 antibody

MBS9602257 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Rabbit Anti-TLE4 Polyclonal Antibody – Cat# MBS9602257

Western Blot (WB) of anti-TLE4 antibody

Western blot analysis of TLE4 using Jurkat whole cell lysates
Rabbit Anti-TLE4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9602257

Other Notes

Small volumes of anti-TLE4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TLE4 purchase

MBS9602257 is an antibody targeting TLE4. Also known as TLE4 Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TLE4. It may not necessarily be applicable to this product.

NCBI GI #
544224628
NCBI GeneID
7091
NCBI Accession #
NP_001269677.1
UniProt Primary Accession #
Q04727
UniProt Secondary Accession #
Q3ZCS1; Q5T1Y2; Q6PCB3; Q9BZ07; Q9BZ08; Q9BZ09; Q9NSL3; Q9ULF9; F8W6T6
UniProt Related Accession #
Q04727
GenBank Nucleotide #
NM_001282748.1
NCBI Official Full Name
transducin-like enhancer protein 4 isoform 1
NCBI Official Synonym Full Names
transducin like enhancer of split 4
NCBI Official Symbol
TLE4
NCBI Official Synonym Symbols
ESG; BCE1; ESG4; GRG4; BCE-1; Grg-4; E(spI)
UniProt Protein Name
Transducin-like enhancer protein 4
UniProt Synonym Protein Names
Grg-4; Groucho-related protein 4
Protein Family
Transducin-like enhancer protein
UniProt Gene Name
TLE4
UniProt Synonym Gene Names
GRG4; KIAA1261
NCBI Protein Information
transducin-like enhancer protein 4

UniProt Comments for TLE4

Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression ().
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Research Articles on TLE4

1. Genes TGFB1, TLE4 and MUC22 are associated with the risk of childhood asthma in Chinese population.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TLE4 antibody

 Products by Pathway  Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 FBXW7 Mutants And NOTCH1 In Cancer Pathway antibodies  FBXW7 Mutants And NOTCH1 In Cancer Pathway Diagram
 NOTCH1 Intracellular Domain Regulates Transcription Pathway antibodies  NOTCH1 Intracellular Domain Regulates Transcription Pathway Diagram
 Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway antibodies  Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By NOTCH Pathway antibodies  Signaling By NOTCH Pathway Diagram
 Signaling By NOTCH1 Pathway antibodies  Signaling By NOTCH1 Pathway Diagram
 Signaling By NOTCH1 HD Domain Mutants In Cancer Pathway antibodies  Signaling By NOTCH1 HD Domain Mutants In Cancer Pathway Diagram
 Signaling By NOTCH1 HD+PEST Domain Mutants In Cancer Pathway antibodies  Signaling By NOTCH1 HD+PEST Domain Mutants In Cancer Pathway Diagram

Diseases associated with anti-TLE4 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >12 publications with TLE4 and Neoplasms
 Lung Diseases Antibodies  >2 publications with TLE4 and Lung Diseases
 Respiratory Tract Diseases Antibodies  >2 publications with TLE4 and Respiratory Tract Diseases
 Intestinal Neoplasms Antibodies  >2 publications with TLE4 and Intestinal Neoplasms
 Carcinoma Antibodies  >2 publications with TLE4 and Carcinoma
 Adenoma Antibodies  >1 publications with TLE4 and Adenoma
 Disease Models, Animal Antibodies  >1 publications with TLE4 and Disease Models, Animal
 Embryo Loss Antibodies  >1 publications with TLE4 and Embryo Loss
 Neoplasms, Experimental Antibodies  >1 publications with TLE4 and Neoplasms, Experimental
 Lymphoma Antibodies  >1 publications with TLE4 and Lymphoma

Organs/Tissues associated with anti-TLE4 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >8 publications with TLE4 and Brain
 Embryonic Tissue Antibodies  >7 publications with TLE4 and Embryonic Tissue
 Nerve Antibodies  >4 publications with TLE4 and Nerve
 Blood Antibodies  >3 publications with TLE4 and Blood
 Eye Antibodies  >3 publications with TLE4 and Eye
 Kidney Antibodies  >3 publications with TLE4 and Kidney
 Lung Antibodies  >2 publications with TLE4 and Lung
 Pancreas Antibodies  >2 publications with TLE4 and Pancreas
 Bone Antibodies  >2 publications with TLE4 and Bone
 Heart Antibodies  >2 publications with TLE4 and Heart
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