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TREF1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6358028

TREF1 encodes a zinc-finger transcriptional regulating protein which interacts with CBP/p300 to regulate the human gene CYP11A1. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
TREF1, Polyclonal Antibody
Full Product Name
TREF1, CT (TRERF1, BCAR2, RAPA, TREP132, Transcriptional-regulating factor 1, Breast cancer anti-estrogen resistance 2, Transcriptional-regulating protein 132, Zinc finger protein rapa, Zinc finger transcription factor TReP-132) (MaxLight 490)
Product Gene Name
anti-TREF1 antibody
Product Synonym Gene Name
TRERF1
Product Synonym Names
TRERF1; BCAR2; RAPA; TREP132; Transcriptional-regulating factor 1; Breast cancer anti-estrogen resistance 2; Transcriptional-regulating protein 132; Zinc finger protein rapa; Zinc finger transcription factor TReP-132
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Specificity
Recognizes human TREF1.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
132,256 Da
Chromosome Location
Chromosome: 6; NC_000006.11 (42192669..42419783, complement). Location: 6p21.1-p12.1
OMIM
610322
3D Structure
ModBase 3D Structure for Q96PN7
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1136-1164 in the C-terminal region of human TREF1.
Conjugate
MaxLight490
Note
Preservative Free

Product Categories/Family for anti-TREF1 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger
Applications Tested/Suitable for anti-TREF1 antibody
Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
Application Notes for anti-TREF1 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-TREF1 antibody

Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS649623. TREF1 (arrow) was detected usingMBS649623.
Rabbit Anti-TREF1 Polyclonal Antibody – Cat# MBS6358028

Immunofluorescence (IF) of anti-TREF1 antibody

Fluorescent image of MCF-7 cell stained withMBS649623. MCF-7 cells were fixed with 4% PFA (20mins), permeabilized with Triton X-100 (0.1%, 10mins), then incubated with MBS649623 (1:25, 1hr at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50mins at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).TREF1 immunoreactivity is localized to Nucleus significantly.
Rabbit Anti-TREF1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6358028

Product References and Citations for anti-TREF1 antibody

1. Gizard, F. Mol. Cell. Biol. 26 (20), 7632-7644 (2006). 2. Gizard, F. Mol. Cell. Biol. 25 (11), 4335-4348 (2005). 3. Beausoleil, S.A. Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135 (2004). 4. Gizard, F. J. Mol. Endocrinol. 32 (2), 557-569 (2004).

Other Notes

Small volumes of anti-TREF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TREF1 purchase

MBS6358028 is an antibody targeting TREF1. Also known as TREF1, CT (TRERF1, BCAR2, RAPA, TREP132, Transcriptional-regulating factor 1, Breast cancer anti-estrogen resistance 2, Transcriptional-regulating protein 132, Zinc finger protein rapa, Zinc finger transcription factor TReP-132) (MaxLight 490). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TREF1. It may not necessarily be applicable to this product.

NCBI GI #
15812226
NCBI GeneID
55809
NCBI Accession #
NP_277037.1
UniProt Primary Accession #
Q96PN7
UniProt Secondary Accession #
Q05GC6; Q7Z6T2; Q7Z6T3; Q9NQ72; Q9NQ73; Q9NUN9
UniProt Related Accession #
Q96PN7
GenBank Nucleotide #
NM_033502.2
NCBI Official Full Name
transcriptional-regulating factor 1
NCBI Official Synonym Full Names
transcriptional regulating factor 1
NCBI Official Symbol
TRERF1
NCBI Official Synonym Symbols
RAPA; BCAR2; TReP-132; HSA277276; dJ139D8.5; RP1-139D8.5
UniProt Protein Name
Transcriptional-regulating factor 1
UniProt Synonym Protein Names
Breast cancer anti-estrogen resistance 2; Transcriptional-regulating protein 132; Zinc finger protein rapa; Zinc finger transcription factor TReP-132
UniProt Gene Name
TRERF1
UniProt Synonym Gene Names
BCAR2; RAPA; TREP132
UniProt Entry Name
TREF1_HUMAN
NCBI Protein Information
transcriptional-regulating factor 1; rapa-1; rapa-2; zinc finger protein rapa; transcriptional regulating protein 132; transcriptional-regulating protein 132; breast cancer anti-estrogen resistance 2; zinc finger transcription factor TReP-132

NCBI Summary for TREF1

This gene encodes a zinc-finger transcriptional regulating protein which interacts with CBP/p300 to regulate the human gene CYP11A1. [provided by RefSeq, Jul 2008]

UniProt Comments for TREF1

TReP-132: Binds DNA and activates transcription of CYP11A1. Interaction with CREBBP and EP300 results in a synergistic transcriptional activation of CYP11A1

Protein type: C2H2-type zinc finger protein; Transcription factor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 6p21.1-p12.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: ligand-dependent nuclear receptor transcription coactivator activity; metal ion binding; chromatin binding; DNA bending activity; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; homeostatic process; positive regulation of transcription, DNA-dependent; multicellular organismal development; cholesterol catabolic process; regulation of hormone biosynthetic process; steroid biosynthetic process
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Research Articles on TREF1

1. Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TREF1 antibody

 Reference Product  PubMed Publications
 NR5A1 antibody  >5 publications with TREF1 and NR5A1
 EP300 antibody  >1 publications with TREF1 and EP300

Diseases associated with anti-TREF1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >6 publications with TREF1 and Neoplasms
 Breast Neoplasms Antibodies  >4 publications with TREF1 and Breast Neoplasms
 Carcinoma Antibodies  >2 publications with TREF1 and Carcinoma
 Kidney Diseases Antibodies  >1 publications with TREF1 and Kidney Diseases

Organs/Tissues associated with anti-TREF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Adrenal Gland Antibodies  >4 publications with TREF1 and Adrenal Gland
 Testis Antibodies  >4 publications with TREF1 and Testis
 Thymus Antibodies  >3 publications with TREF1 and Thymus
 Lymph Node Antibodies  >1 publications with TREF1 and Lymph Node
 Ganglia Antibodies  >1 publications with TREF1 and Ganglia
 Mammary Gland Antibodies  >1 publications with TREF1 and Mammary Gland
 Placenta Antibodies  >1 publications with TREF1 and Placenta
 Kidney Antibodies  >1 publications with TREF1 and Kidney
 Brain Antibodies  >1 publications with TREF1 and Brain
 Embryonic Tissue Antibodies  >1 publications with TREF1 and Embryonic Tissue
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