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TRIM4 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420359

MBS420359 is an antibody targeting TRIM4. Also known as Goat anti-TRIM4 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TRIM4, Polyclonal Antibody
Full Product Name
Goat anti-TRIM4 Antibody
Product Gene Name
Product Synonym Names
TRIM4; RNF87; tripartite motif-containing 4; tripartite motif protein 4; tripartite motif protein TRIM4; tripartite motif protein TRIM4 isoform alpha; tripartite motif protein 4; TRIM4 antibody; RNF87 antibody; tripartite motif-containing 4 antibody; tripartite motif protein 4 antibody; tripartite motif protein TRIM4 antibody; tripartite motif protein TRIM4 isoform alpha antibody; tripartite motif protein 4 antibody
Immunogen Sequence
ASLVIPPVTD RK
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
TRIM4 peptide (MBS425775) is used for blocking the activity of TRIM4 antibody (MBS420359)
Molecular Weight
34,194 Da
Immunogen
Peptide with sequence C-ASLVIPPVTDRK, from the C Terminus of the protein sequence according to NP_148977.1; NP_149082.1.
Epitope
C Terminus
Note
This antibody is expected to recognise both reported human isoforms (as represented by NP_148977 and NP_149082).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-TRIM4 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-TRIM4 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 55-60kDa band observed in HepG2 whole cell lysate (predicted MW of 58kDa and 55kDa according to NP_148977 and NP_149082). Recommended for use at 1ug/ml.

Western Blot (WB) of anti-TRIM4 antibody

Staining (1ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Goat Anti-TRIM4 Polyclonal Antibody – Cat# MBS420359

Other Notes

Small volumes of anti-TRIM4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TRIM4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tripartite motif-containing protein 4 isoform alpha
NCBI Official Synonym Full Names
tripartite motif containing 4
NCBI Official Symbol
TRIM4
NCBI Official Synonym Symbols
RNF87
UniProt Protein Name
E3 ubiquitin-protein ligase TRIM4
UniProt Synonym Protein Names
RING finger protein 87; Tripartite motif-containing protein 4
UniProt Gene Name
TRIM4
UniProt Synonym Gene Names
RNF87
UniProt Entry Name
TRIM4_HUMAN
NCBI Protein Information
E3 ubiquitin-protein ligase TRIM4

NCBI Summary for TRIM4

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternatively spliced transcript variants that encode different isoforms have been described.[provided by RefSeq, Jul 2010]
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UniProt Comments for TRIM4

TRIM4: is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternatively spliced transcript variants that encode different isoforms have been described.[provided by RefSeq, Jul 2010]

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 7q22-q31.1

Cellular Component: cytoplasm; plasma membrane
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TRIM4 antibody

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