Goat Anti-TRPC4AP Polyclonal Antibody – Cat# MBS421770

(1ug/ml) staining of U937 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TRPC4AP Antibody – Goat Polyclonal

Name: TRPC4AP Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS421770
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421770

MBS421770 is an antibody targeting TRPC4AP. Also known as Goat anti-TRPC4AP Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

TRPC4AP, Polyclonal Antibody

Full Product Name

Goat anti-TRPC4AP Antibody

Product Gene Name

anti-TRPC4AP antibody

Product Synonym Names

TRPC4AP; transient receptor potential cation channel, subfamily C, member 4 associated protein; C20orf188; TRRP4AP; TRUSS; TRP4-associated protein; TRPC4-associated protein; short transient receptor potential channel 4 associated protein; tumor necrosis; TRPC4AP antibody; transient receptor potential cation channel; subfamily C; member 4 associated protein antibody; C20orf188; TRRP4AP; TRUSS antibody; TRP4-associated protein antibody; TRPC4-associated protein antibody; short transient receptor potential channel 4 associated protein antibody; tumor necrosis factor receptor-associated ubiquitous scaffolding and signaling protein antibody

Immunogen Sequence

KLTQETTYPN TYIFD

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

TRPC4AP peptide (MBS426966) is used for blocking the activity of TRPC4AP antibody (MBS421770)

Molecular Weight

90,043 Da

OMIM

608430

Immunogen

Peptide with sequence C-KLTQETTYPNTYIFD, from the internal region of the protein sequence according to NP_056453.1; NP_955400.1.

Epitope

Internal region

Note

This antibody is expected to recognize both reported isoforms (NP_955400.1; NP_056453.1).

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-TRPC4AP antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-TRPC4AP antibody

Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Approx 85kDa band observed in lysates of cell line U937 (calculated MW of 90. 0kDa according to human NP_056453.1). Recommended concentration: 1-3ug/ml.

Other Notes

Small volumes of anti-TRPC4AP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TRPC4AP. It may not necessarily be applicable to this product.

NCBI GI #

18158416

NCBI GeneID

26133

NCBI Accession #

NP_056453.1

NCBI Related Accession #

NP_955400.1

UniProt Secondary Accession #

Q96H82; Q9BVB8; Q9H429; Q9UFS6; E1P5Q0; E1P5Q1

UniProt Related Accession #

Q8TEL6

GenBank Nucleotide #

NM_015638.2

NCBI Official Full Name

short transient receptor potential channel 4-associated protein isoform a

NCBI Official Synonym Full Names

transient receptor potential cation channel subfamily C member 4 associated protein

NCBI Official Symbol

TRPC4AP

NCBI Official Synonym Symbols

TRUSS; TRRP4AP; PPP1R158; C20orf188

UniProt Protein Name

Short transient receptor potential channel 4-associated protein

UniProt Synonym Protein Names

Protein TAP1; TNF-receptor ubiquitous scaffolding/signaling protein; Protein TRUSS

Protein Family

Short transient receptor potential channel 4-associated protein

UniProt Gene Name

TRPC4AP

UniProt Synonym Gene Names

C20orf188; TRRP4AP; Trp4-associated protein; Trpc4-associated protein; Protein TRUSS

UniProt Entry Name

TP4AP_HUMAN

NCBI Protein Information

short transient receptor potential channel 4-associated protein

UniProt Comments for TRPC4AP

TRPC4AP: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. The DCX(TRUSS) complex specifically mediates the polyubiquitination and subsequent degradation of MYC. Also participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. Involved in JNK activation via its interaction with TRAF2. Also involved in elevation of endoplasmic reticulum Ca(2+) storage reduction in response to CHRM1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: plasma membrane

Molecular Function: calcium channel activity; phosphatase binding; protein binding

Biological Process: protein ubiquitination; ubiquitin-dependent protein catabolic process

Research Articles on TRPC4AP

1. The latent classification method of analysis showed that the TRPC4AP H1 haplotype was characteristic of Alzheimer's patients, with ages-of-onset between 66 and 80 years.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TRPC4AP antibody

Products by Pathway	Pathway Diagram
Ion Channel Transport Pathway antibodies	Ion Channel Transport Pathway Diagram
Stimuli-sensing Channels Pathway antibodies	Stimuli-sensing Channels Pathway Diagram
TNF-alpha/NF-kB Signaling Pathway antibodies	TNF-alpha/NF-kB Signaling Pathway Diagram
TRP Channels Pathway antibodies	TRP Channels Pathway Diagram
Transmembrane Transport Of Small Molecules Pathway antibodies	Transmembrane Transport Of Small Molecules Pathway Diagram

Diseases associated with anti-TRPC4AP antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>3 publications with TRPC4AP and Necrosis
Liver Neoplasms Antibodies	>1 publications with TRPC4AP and Liver Neoplasms

Organs/Tissues associated with anti-TRPC4AP antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>2 publications with TRPC4AP and Brain
Liver Antibodies	>1 publications with TRPC4AP and Liver
Skin Antibodies	>1 publications with TRPC4AP and Skin
Embryonic Tissue Antibodies	>1 publications with TRPC4AP and Embryonic Tissue