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TTC39A Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1759472

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TTC39A, Polyclonal Antibody
Full Product Name
Anti-TTC39A Antibody Picoband
Product Gene Name
anti-TTC39A antibody
Product Synonym Names
Transmembrane protein 240; kelch repeat and BTB domain containing 2; Kelch repeat and BTB domain-containing protein 2; BTB and kelch domain-containing protein 1; KBTBD2; BKLHD1; KIAA1489
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
69,778 Da
3D Structure
ModBase 3D Structure for Q5SRH9
Immunogen
E coli-derived human TTC39A recombinant protein (Position: E18-E489).
Subcellular Localization
Cul3-RING ubiquitin ligase complex
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.

Product Categories/Family for anti-TTC39A antibody

Primary Antibodies; Cell Biology
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TTC39A antibody
Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-TTC39A antibody
WB: 0.25-0.5ug/ml, Human
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-TTC39A antibody

Figure 1. Western blot analysis of TTC39A using anti-TTC39A antibody (MBS1759472).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTC39A antigen affinity purified polyclonal antibody (#MBS1759472) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for TTC39A at approximately 72 kDa. The expected band size for TTC39A is at 70 kDa.
Rabbit Anti-TTC39A Polyclonal Antibody – Cat# MBS1759472

Flow Cytometry (FC/FACS) of anti-TTC39A antibody

Figure 2. Flow Cytometry analysis of 293T cells using anti-TTC39A antibody (MBS1759472).
Overlay histogram showing 293T cells stained with MBS1759472 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTC39A Antibody (MBS1759472, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-TTC39A Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1759472

Product References and Citations for anti-TTC39A antibody

1. Gross, M. B. Personal Communication. Baltimore, Md. 5/24/2022.2. Seki, N., Ohira, M., Nagase, T., Ishikawa, K., Miyajima, N., Nakajima, D., Nomura, N., Ohara, O. Characterization of cDNA clones in size-fractionated cDNA libraries from human brain. DNA Res. 4: 345-349, 1997.

Other Notes

Small volumes of anti-TTC39A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TTC39A purchase

MBS1759472 is an antibody targeting TTC39A. Also known as Anti-TTC39A Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TTC39A. It may not necessarily be applicable to this product.

NCBI GI #
122937436
NCBI GeneID
22996
NCBI Accession #
NP_001073963.1
UniProt Primary Accession #
Q5SRH9
UniProt Secondary Accession #
O43417; O75040; Q5SRH5; Q5SRH6; Q5SRH7; Q5SRH8; Q5SRI0; Q5SRI1; B7Z782; E7EQY9; G3XAF8
UniProt Related Accession #
Q5SRH9
GenBank Nucleotide #
NM_001080494.3
NCBI Official Full Name
tetratricopeptide repeat protein 39A isoform 2
NCBI Official Synonym Full Names
tetratricopeptide repeat domain 39A
NCBI Official Symbol
TTC39A
NCBI Official Synonym Symbols
DEME-6; C1orf34
UniProt Protein Name
Tetratricopeptide repeat protein 39A
UniProt Synonym Protein Names
Differentially expressed in MCF7 with estradiol protein 6; DEME-6
Protein Family
Tetratricopeptide repeat protein
UniProt Gene Name
TTC39A
UniProt Synonym Gene Names
C1orf34; KIAA0452; TPR repeat protein 39A; DEME-6
UniProt Entry Name
TT39A_HUMAN
NCBI Protein Information
tetratricopeptide repeat protein 39A; TPR repeat protein 39A; differentially expressed in MCF7 with estradiol protein 6

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-TTC39A antibody

 Disease Name  Pubmed Publications
 Skin Diseases Antibodies  >1 publications with TTC39A and Skin Diseases
 Breast Neoplasms Antibodies  >1 publications with TTC39A and Breast Neoplasms
 Neoplasms Antibodies  >1 publications with TTC39A and Neoplasms
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