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UBC9 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6504411

MBS6504411 is an antibody targeting UBC9. Also known as UBC9, NT (SUMO-conjugating Enzyme UBC9, SUMO-protein Ligase, Ubiquitin-conjugating Enzyme E2 I, Ubiquitin-protein Ligase I, Ubiquitin Carrier Protein I, Ubiquitin Carrier Protein 9, p18, UBE2I, UBC9,… More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
UBC9, Polyclonal Antibody
Full Product Name
UBC9, NT (SUMO-conjugating Enzyme UBC9, SUMO-protein Ligase, Ubiquitin-conjugating Enzyme E2 I, Ubiquitin-protein Ligase I, Ubiquitin Carrier Protein I, Ubiquitin Carrier Protein 9, p18, UBE2I, UBC9, UBCE9) (AP)
Product Gene Name
anti-UBC9 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Specificity
Recognizes human UBC9.
Preparation and Storage
May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
18 kDa (158 aa), confirmed by MALDI-TOF.
OMIM
601661
3D Structure
ModBase 3D Structure for P63279
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human UBC19, UniProt Accession #P63279.
Conjugate
AP
Note
Preservative Free

Product Categories/Family for anti-UBC9 antibody

Antibodies; Ubiquitin Ligases
Applications Tested/Suitable for anti-UBC9 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-UBC9 antibody
IHC: 1:50-1:100
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-UBC9 antibody

Western blot analysis of A2058 cell line lysates (35ug/lane) usingMBS626003. UBE2I (arrow) was detected using theMBS616215.
Rabbit Anti-UBC9 Polyclonal Antibody – Cat# MBS6504411

Immunohistochemistry (IHC) of anti-UBC9 antibody

Formalin-fixed and paraffin-embedded human cancer tissue usingMBS626003, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-UBC9 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6504411

Western Blot (WB) of anti-UBC9 antibody

Western blot analysis of 293 cell lysates (2ug/lane) using MBS626003 on either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2I gene.
Rabbit Anti-UBC9 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6504411

Product References and Citations for anti-UBC9 antibody

1. Biochem Biophys Res Commun. 2002 Aug 30;296(4):870-6. 2. Genomics. 1996 Oct 15;37(2):183-6. 3. Cytogenet Cell Genet. 1996;75(4):222-3. 4. Cytogenet Cell Genet. 1996;72(1):86-9.

Other Notes

Small volumes of anti-UBC9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for UBC9. It may not necessarily be applicable to this product.

NCBI GI #
4507785
NCBI GeneID
7329
NCBI Accession #
NP_003336
UniProt Primary Accession #
P63279
UniProt Secondary Accession #
P50550; Q15698; Q59GX1; Q86VB3; D3DU69
UniProt Related Accession #
P63279
GenBank Nucleotide #
NM_003345.4
NCBI Official Full Name
SUMO-conjugating enzyme UBC9
NCBI Official Synonym Full Names
ubiquitin conjugating enzyme E2 I
NCBI Official Symbol
UBE2I
NCBI Official Synonym Symbols
P18; UBC9; C358B7.1
UniProt Protein Name
SUMO-conjugating enzyme UBC9
UniProt Synonym Protein Names
SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I; p18
Protein Family
SUMO-conjugating enzyme
UniProt Gene Name
UBE2I
UniProt Synonym Gene Names
UBC9; UBCE9
UniProt Entry Name
UBC9_HUMAN
NCBI Protein Information
SUMO-conjugating enzyme UBC9

NCBI Summary for UBC9

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
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Research Articles on UBC9

1. Ubc9 is an essential regulator of ADAP where it is required for TCR-induced membrane recruitment of the small GTPase Rap1 and its effector protein RapL and for activation of the small GTPase Rac1 in T cell adhesion.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-UBC9 antibody

 Reference Product  PubMed Publications
 SUMO2 antibody  >3 publications with UBC9 and SUMO2
 SUMO1 antibody  >3 publications with UBC9 and SUMO1
 SUMO3 antibody  >1 publications with UBC9 and SUMO3
 PIAS1 antibody  >1 publications with UBC9 and PIAS1
 UBA2 antibody  >1 publications with UBC9 and UBA2
 TP53 antibody  >1 publications with UBC9 and TP53

Pathways associated with anti-UBC9 antibody

 Products by Pathway  Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Coregulation Of Androgen Receptor Activity Pathway antibodies  Coregulation Of Androgen Receptor Activity Pathway Diagram
 DNA Double Strand Break Response Pathway antibodies  DNA Double Strand Break Response Pathway Diagram
 DNA Double-Strand Break Repair Pathway antibodies  DNA Double-Strand Break Repair Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 Formation Of Incision Complex In GG-NER Pathway antibodies  Formation Of Incision Complex In GG-NER Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram

Diseases associated with anti-UBC9 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >4 publications with UBC9 and Neoplasms
 Nervous System Diseases Antibodies  >2 publications with UBC9 and Nervous System Diseases
 Brain Diseases Antibodies  >1 publications with UBC9 and Brain Diseases
 Carcinoma Antibodies  >1 publications with UBC9 and Carcinoma

Organs/Tissues associated with anti-UBC9 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with UBC9 and Brain
 Heart Antibodies  >2 publications with UBC9 and Heart
 Blood Antibodies  >2 publications with UBC9 and Blood
 Bone Marrow Antibodies  >1 publications with UBC9 and Bone Marrow
 Muscle Antibodies  >1 publications with UBC9 and Muscle
 Liver Antibodies  >1 publications with UBC9 and Liver
 Vascular Antibodies  >1 publications with UBC9 and Vascular
 Nerve Antibodies  >1 publications with UBC9 and Nerve
 Testis Antibodies  >1 publications with UBC9 and Testis
 Ovary Antibodies  >1 publications with UBC9 and Ovary
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