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UBE2D4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6360652

UBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
UBE2D4, Polyclonal Antibody
Full Product Name
UBE2D4, CT (UBE2D4, UBCH5D, Ubiquitin-conjugating enzyme E2 D4, HBUCE1, Ubiquitin carrier protein D4, Ubiquitin-protein ligase D4) (APC)
Product Gene Name
Product Synonym Names
UBE2D4; UBCH5D; Ubiquitin-conjugating enzyme E2 D4; HBUCE1; Ubiquitin carrier protein D4; Ubiquitin-protein ligase D4
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
16,649 Da
3D Structure
ModBase 3D Structure for Q9Y2X8
Immunogen
UBE2D4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the C-terminal region of human UBE2D4.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-UBE2D4 antibody

Applications Tested/Suitable for anti-UBE2D4 antibody
Western Blot (WB), FLISA
Application Notes for anti-UBE2D4 antibody
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-UBE2D4 antibody

UBE2D4 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the UBE2D4 antibody detected the UBE2D4 protein (arrow).
Rabbit Anti-UBE2D4 Polyclonal Antibody – Cat# MBS6360652

Product References and Citations for anti-UBE2D4 antibody

Markson, G., et al. Genome Res. 19(10):1905-1911(2009). van Wijk, S.J., et al. Mol. Syst. Biol. 5, 295 (2009): Lamesch, P., et al. Genomics 89(3):307-315(2007). Colland, F., et al. Genome Res. 14(7):1324-1332(2004).

Other Notes

Small volumes of anti-UBE2D4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UBE2D4 purchase

MBS6360652 is an antibody targeting UBE2D4. Also known as UBE2D4, CT (UBE2D4, UBCH5D, Ubiquitin-conjugating enzyme E2 D4, HBUCE1, Ubiquitin carrier protein D4, Ubiquitin-protein ligase D4) (APC). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for UBE2D4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ubiquitin-conjugating enzyme E2 D4
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2D 4 (putative)
NCBI Official Symbol
UBE2D4
NCBI Official Synonym Symbols
HBUCE1
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 D4
UniProt Synonym Protein Names
HBUCE1; Ubiquitin carrier protein D4; Ubiquitin-protein ligase D4
UniProt Gene Name
UBE2D4
UniProt Synonym Gene Names
UBCH5D
UniProt Entry Name
UB2D4_HUMAN
NCBI Protein Information
ubiquitin-conjugating enzyme E2 D4; ubiquitin carrier protein D4; ubiquitin-conjugating enzyme HBUCE1; ubiquitin-protein ligase D4

UniProt Comments for UBE2D4

UBE2D4: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Ligase; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 7p13

Molecular Function: protein binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: protein ubiquitination
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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