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UBQLN2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8124129

Ubiquilin-2 (UBQLN2) is a 66?kDa protein member of the ubiquilin family. UBQLN2 has a ubiquitin-like domain (UBL) in its N-terminal that can interact with the proteasome, and a ubiquitin-associated domain (UBA) on the C-terminal which recognizes the… More details

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Product Overview

Online Datasheet in PDF
Product Name
UBQLN2, Polyclonal Antibody
Full Product Name
Anti-UBQLN2 Antibody, Rabbit Polyclonal
Product Gene Name
anti-UBQLN2 antibody
Product Synonym Names
UBQLN2 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-UBQLN2 Antibody; Anti-UBQLN2 Antibody
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Purity/Purification
Protein A & Antigen Affinity
Specificity
Human UBQLN2
Preparation and Storage
This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Conjugation
Unconjugated
Immunogen
E Coli-derived Human UBQLN2 fragment
Preparation
Produced in rabbits immunized with E Coli-derived Human UBQLN2 fragment, and purified by antigen affinity chromatography.
Applications Tested/Suitable for anti-UBQLN2 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes for anti-UBQLN2 antibody
WB: 1:500-1:1000
IHC-P: 1:50-1:200
IP: 1-5 uL/mg of lysate

Western Blot (WB) of anti-UBQLN2 antibody

Anti-UBQLN2 rabbit polyclonal antibody at 1:500 dilution Lane A: 293T Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:66 kDa Observed band size:70 kDa
Rabbit Anti-UBQLN2 Polyclonal Antibody – Cat# MBS8124129

Immunohistochemistry (IHC)-Paraffin of anti-UBQLN2 antibody

Immunochemical staining of human UBQLN2 in human breast with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Rabbit Anti-UBQLN2 Polyclonal Antibody, Immunohistochemistry (IHC)-Paraffin – Cat# MBS8124129

Immunohistochemistry (IHC)-Paraffin of anti-UBQLN2 antibody

Immunochemical staining of human UBQLN2 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Rabbit Anti-UBQLN2 Polyclonal Antibody, Immunohistochemistry (IHC)-Paraffin – Cat# MBS8124129

Immunoprecipitation (IP) of anti-UBQLN2 antibody

UBQLN2 was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate Lane B:0.5 mg Jurkat Whole Cell Lysate Lane C:0.5 mg HepG2 Whole Cell Lysate 4 uL anti- UBQLN2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti- UBQLN2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 66kDa Observed band size :66 kDa
Rabbit Anti-UBQLN2 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS8124129

Product References and Citations for anti-UBQLN2 antibody

Siddique N, et al. (1993) Amyotrophic lateral sclerosis overview. In: Adam MP, Ardinger HH, Pagon RA, Wallace SE, Bean LJH, Stephens K, Amemiya A (eds) Genereviews(). University of Washington, SeattleRenton AE, et al. (2014) State of play in amyotrophic lateral sclerosis genetics. Nat Neurosci 17 (1): 17-23.Renaud L, et al. (2019) Key role of ubqln2 in pathogenesis of amyotrophic lateral sclerosis and frontotemporal dementia. Acta Neuropathol Commun 7 (1): 103.

Other Notes

Small volumes of anti-UBQLN2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UBQLN2 purchase

MBS8124129 is an antibody targeting UBQLN2. Also known as Anti-UBQLN2 Antibody, Rabbit Polyclonal. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for UBQLN2. It may not necessarily be applicable to this product.

Protein Family
Ubiquilin

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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