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VPS52 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6362777

This gene encodes a protein that is similar to the yeast suppressor of actin mutations 2 gene. The yeast protein forms a subunit of the tetrameric Golgi-associated retrograde protein complex that is involved in vesicle trafficking from from both early and… More details

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Product Overview

Online Datasheet in PDF
Product Name
VPS52, Polyclonal Antibody
Full Product Name
VPS52, CT (VPS52, SACM2L, Vacuolar protein sorting-associated protein 52 homolog, SAC2 suppressor of actin mutations 2-like protein) (FITC)
Product Gene Name
anti-VPS52 antibody
Product Synonym Names
VPS52; SACM2L; Vacuolar protein sorting-associated protein 52 homolog; SAC2 suppressor of actin mutations 2-like protein
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
82,221 Da
Chromosome Location
Chromosome: 6; NC_000006.11 (33218049..33239723, complement). Location: 6p21.3
OMIM
603443
3D Structure
ModBase 3D Structure for Q8N1B4
Immunogen
VPS52 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 613-641 amino acids from the C-terminal region of human VPS52.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-VPS52 antibody

Antibodies; Actin Related Proteins
Applications Tested/Suitable for anti-VPS52 antibody
Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-VPS52 antibody
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-VPS52 antibody

VPS52 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the VPS52 antibody detected the VPS52 protein (arrow).
Rabbit Anti-VPS52 Polyclonal Antibody – Cat# MBS6362777

Immunohistochemistry (IHC) of anti-VPS52 antibody

VPS52 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the VPS52 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-VPS52 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6362777

Flow Cytometry (FC/FACS) of anti-VPS52 antibody

VPS52 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-VPS52 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6362777

Immunofluorescence (IF) of anti-VPS52 antibody

Confocal immunofluorescent analysis of VPS52 Antibody (C-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-VPS52 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6362777

Product References and Citations for anti-VPS52 antibody

Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009): Perez-Victoria, F.J., et al. Mol. Cell. Biol. 29(19):5251-5263(2009). Perez-Victoria, F.J., et al. Mol. Biol. Cell 19(6):2350-2362(2008). Liewen, H., et al. Exp. Cell Res. 306(1):24-34(2005). Mungall, A.J., et al. Nature 425(6960):805-811(2003).

Other Notes

Small volumes of anti-VPS52 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VPS52 purchase

MBS6362777 is an antibody targeting VPS52. Also known as VPS52, CT (VPS52, SACM2L, Vacuolar protein sorting-associated protein 52 homolog, SAC2 suppressor of actin mutations 2-like protein) (FITC). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for VPS52. It may not necessarily be applicable to this product.

NCBI GI #
73747799
NCBI GeneID
6293
NCBI Accession #
NP_072047.4
UniProt Primary Accession #
Q8N1B4
UniProt Secondary Accession #
Q53GR4; Q5JPA0; Q5SQW1; Q8IUN6; Q9NPT5; A2BF38; B0UZZ4
UniProt Related Accession #
Q8N1B4
GenBank Nucleotide #
NM_022553.4
NCBI Official Full Name
vacuolar protein sorting-associated protein 52 homolog
NCBI Official Synonym Full Names
vacuolar protein sorting 52 homolog (S. cerevisiae)
NCBI Official Symbol
VPS52
NCBI Official Synonym Symbols
ARE1; SAC2; SACM2L; RP5-1033B10; dJ1033B10.5
UniProt Protein Name
Vacuolar protein sorting-associated protein 52 homolog
UniProt Synonym Protein Names
SAC2 suppressor of actin mutations 2-like protein
Protein Family
Vacuolar protein sorting-associated protein
UniProt Gene Name
VPS52
UniProt Synonym Gene Names
SACM2L
UniProt Entry Name
VPS52_HUMAN
NCBI Protein Information
vacuolar protein sorting-associated protein 52 homolog; SAC2 suppressor of actin mutations 2-like protein

UniProt Comments for VPS52

Function: May be involved in retrograde transport of early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Ref.6 Ref.7

Subunit structure: Component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. Interacts with RAB6A and STX10. Ref.6 Ref.8

Subcellular location: Golgi apparatus › trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein Ref.6 Ref.7.

Sequence similarities: Belongs to the VPS52 family.

Sequence caution: The sequence CAI95619.1 differs from that shown. Reason: Erroneous gene model prediction.
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Research Articles on VPS52

1. Golgi-associated retrograde protein orchestrates retrograde transport from endosomes to the trans-Golgi network by promoting vesicle tethering and assembly of SNARE complexes in consecutive, independent steps.[Golgi associated retrograde protein]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-VPS52 antibody

 Reference Product  PubMed Publications
 VPS54 antibody  >9 publications with VPS52 and VPS54
 VPS53 antibody  >8 publications with VPS52 and VPS53
 RPS18 antibody  >1 publications with VPS52 and RPS18

Diseases associated with anti-VPS52 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >3 publications with VPS52 and Inflammation
 Nervous System Diseases Antibodies  >2 publications with VPS52 and Nervous System Diseases
 Brain Diseases Antibodies  >1 publications with VPS52 and Brain Diseases
 Weight Loss Antibodies  >1 publications with VPS52 and Weight Loss
 Gastrointestinal Diseases Antibodies  >1 publications with VPS52 and Gastrointestinal Diseases
 Seizures Antibodies  >1 publications with VPS52 and Seizures
 Drug-Induced Liver Injury Antibodies  >1 publications with VPS52 and Drug-Induced Liver Injury

Organs/Tissues associated with anti-VPS52 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >2 publications with VPS52 and Liver
 Brain Antibodies  >2 publications with VPS52 and Brain
 Embryonic Tissue Antibodies  >1 publications with VPS52 and Embryonic Tissue
 Blood Antibodies  >1 publications with VPS52 and Blood
 Connective Tissue Antibodies  >1 publications with VPS52 and Connective Tissue
 Skin Antibodies  >1 publications with VPS52 and Skin
 Testis Antibodies  >1 publications with VPS52 and Testis
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