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XPA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6506294

MBS6506294 is an antibody targeting XPA. Also known as XPA, CT (Xeroderma Pigmentosum Complementation Group A, Xeroderma Pigmentosum Group A Complementing Protein, Xpac, DNA Repair Protein Complementing XP A Cells, DNA Repair Protein Complementing XPA Cells,… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
XPA, Polyclonal Antibody
Full Product Name
XPA, CT (Xeroderma Pigmentosum Complementation Group A, Xeroderma Pigmentosum Group A Complementing Protein, Xpac, DNA Repair Protein Complementing XP A Cells, DNA Repair Protein Complementing XPA Cells, DNA Repair Protein Complementing XP-A Cells, Excisi
Product Gene Name
anti-XPA antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Immunoaffinity chromatography.
Specificity
Recognizes human XPA.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
31,368 Da
Chromosome Location
Chromosome: 9; NC_000009.11 (100437191..100459691, complement). Location: 9q22.3
OMIM
278700
3D Structure
ModBase 3D Structure for P23025
Immunogen
Synthetic peptide selected from the C-term region of human XPA (KLH).
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-XPA antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger
Applications Tested/Suitable for anti-XPA antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-XPA antibody
WB: 1:100-1:500
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-XPA antibody

Western blot analysis of XPA Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). XPA (arrow) was detected using the purified Pab.
Rabbit Anti-XPA Polyclonal Antibody – Cat# MBS6506294

Immunohistochemistry (IHC) of anti-XPA antibody

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with XPA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-XPA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6506294

Flow Cytometry (FC/FACS) of anti-XPA antibody

XPA Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-XPA Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6506294

Product References and Citations for anti-XPA antibody

1. States J.C., et.al., Hum. Mutat. 12:103-113(1998).

Other Notes

Small volumes of anti-XPA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for XPA. It may not necessarily be applicable to this product.

NCBI GI #
4507937
NCBI GeneID
7507
NCBI Accession #
NP_000371.1
UniProt Primary Accession #
P23025
UniProt Secondary Accession #
Q5T1U9; Q6LCW7; Q6LD02
UniProt Related Accession #
P23025
GenBank Nucleotide #
NM_000380.3
NCBI Official Full Name
DNA repair protein complementing XP-A cells
NCBI Official Synonym Full Names
xeroderma pigmentosum, complementation group A
NCBI Official Symbol
XPA
NCBI Official Synonym Symbols
XP1; XPAC
UniProt Protein Name
DNA repair protein complementing XP-A cells
UniProt Synonym Protein Names
Xeroderma pigmentosum group A-complementing protein
Protein Family
DNA repair protein
UniProt Gene Name
XPA
UniProt Synonym Gene Names
XPAC
UniProt Entry Name
XPA_HUMAN
NCBI Protein Information
DNA repair protein complementing XP-A cells; excision repair-controlling; xeroderma pigmentosum group A-complementing protein

NCBI Summary for XPA

This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]
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UniProt Comments for XPA

XPA: Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. Interacts with GPN1 and RPA1. Interacts (via N-terminus) with CEP164 upon UV irradiation. Interacts with HERC2. Expressed in various cell lines and in skin fibroblasts. Belongs to the XPA family.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 9q22.3

Cellular Component: nucleoplasm; Golgi apparatus; intercellular bridge; DNA replication factor A complex; cytoplasm; nucleus

Molecular Function: protein domain specific binding; protein binding; protein homodimerization activity; metal ion binding; damaged DNA binding

Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; nucleotide-excision repair; response to toxin; multicellular organism growth; nucleotide-excision repair, DNA damage removal; response to oxidative stress; DNA repair; response to UV

Disease: Xeroderma Pigmentosum, Complementation Group A
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Research Articles on XPA

1. APIM in XPA is a functional PCNA interacting motif.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-XPA antibody

 Reference Product  PubMed Publications
 ERCC1 antibody  >155 publications with XPA and ERCC1
 RPA1 antibody  >61 publications with XPA and RPA1
 ERCC6 antibody  >29 publications with XPA and ERCC6
 DDB2 antibody  >22 publications with XPA and DDB2
 ATR antibody  >20 publications with XPA and ATR
 ATM antibody  >20 publications with XPA and ATM
 PRKDC antibody  >6 publications with XPA and PRKDC
 XAB2 antibody  >4 publications with XPA and XAB2
 RPA2 antibody  >4 publications with XPA and RPA2
 GTF2H1 antibody  >2 publications with XPA and GTF2H1

Pathways associated with anti-XPA antibody

 Products by Pathway  Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 Dual Incision Reaction In GG-NER Pathway antibodies  Dual Incision Reaction In GG-NER Pathway Diagram
 Formation Of Incision Complex In GG-NER Pathway antibodies  Formation Of Incision Complex In GG-NER Pathway Diagram
 Global Genomic NER (GG-NER) Pathway antibodies  Global Genomic NER (GG-NER) Pathway Diagram
 Nucleotide Excision Repair Pathway antibodies  Nucleotide Excision Repair Pathway Diagram
 Nucleotide Excision Repair Pathway antibodies  Nucleotide Excision Repair Pathway Diagram
 Nucleotide Excision Repair Pathway antibodies  Nucleotide Excision Repair Pathway Diagram

Diseases associated with anti-XPA antibody

 Disease Name  Pubmed Publications
 Xeroderma Pigmentosum Antibodies  >657 publications with XPA and Xeroderma Pigmentosum
 Neoplasms Antibodies  >427 publications with XPA and Neoplasms
 Precancerous Conditions Antibodies  >242 publications with XPA and Precancerous Conditions
 Skin Neoplasms Antibodies  >71 publications with XPA and Skin Neoplasms
 Drug Toxicity Antibodies  >68 publications with XPA and Drug Toxicity
 Nervous System Diseases Antibodies  >58 publications with XPA and Nervous System Diseases
 Disease Models, Animal Antibodies  >57 publications with XPA and Disease Models, Animal
 Lung Neoplasms Antibodies  >42 publications with XPA and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >41 publications with XPA and Neoplasms, Experimental
 Liver Neoplasms Antibodies  >22 publications with XPA and Liver Neoplasms

Organs/Tissues associated with anti-XPA antibody

 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >159 publications with XPA and Skin
 Lung Antibodies  >67 publications with XPA and Lung
 Liver Antibodies  >52 publications with XPA and Liver
 Blood Antibodies  >52 publications with XPA and Blood
 Brain Antibodies  >30 publications with XPA and Brain
 Kidney Antibodies  >14 publications with XPA and Kidney
 Bone Antibodies  >14 publications with XPA and Bone
 Ganglia Antibodies  >11 publications with XPA and Ganglia
 Mouth Antibodies  >6 publications with XPA and Mouth
 Prostate Antibodies  >6 publications with XPA and Prostate
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