• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

XRCC6 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6364126

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of… More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
XRCC6, Polyclonal Antibody
Full Product Name
XRCC6, ID (XRCC6, G22P1, X-ray repair cross-complementing protein 6, 5'-deoxyribose-5-phosphate lyase Ku70, 70kD subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70kD subunit, CTC box-binding factor 75kD subunit
Product Gene Name
anti-XRCC6 antibody
Product Synonym Names
XRCC6; G22P1; X-ray repair cross-complementing protein 6; 5'-deoxyribose-5-phosphate lyase Ku70; 70kD subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70kD subunit; CTC box-binding factor 75kD subunit; DNA repair protein XRCC6; Lupus Ku autoantigen protein p70; Thyroid-lupus autoantigen; X-ray repair complementing defective repair in Chinese hamster cells 6
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
69,843 Da
Chromosome Location
Chromosome: 22; NC_000022.10 (42017295..42060052). Location: 22q13.2
OMIM
152690
3D Structure
ModBase 3D Structure for P12956
Immunogen
XRCC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-262 amino acids from the Central region of human XRCC6.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-XRCC6 antibody

Antibodies; DNA Replication; Repair
Applications Tested/Suitable for anti-XRCC6 antibody
Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-XRCC6 antibody
FC/FACS: 1:10-50
FLISA: 1:1,000
IF: 1:10-50
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-XRCC6 antibody

XRCC6 Antibody (Center) western blot analysis in 293,A549 cell line lysates (35ug/lane).This demonstrates the XRCC6 antibody detected the XRCC6 protein (arrow).
Rabbit Anti-XRCC6 Polyclonal Antibody – Cat# MBS6364126

Immunohistochemistry (IHC) of anti-XRCC6 antibody

XRCC6 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the XRCC6 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-XRCC6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6364126

Flow Cytometry (FC/FACS) of anti-XRCC6 antibody

XRCC6 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-XRCC6 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6364126

Immunofluorescence (IF) of anti-XRCC6 antibody

Confocal immunofluorescent analysis of XRCC6 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-XRCC6 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6364126

Product References and Citations for anti-XRCC6 antibody

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Liu, Y., et al. Carcinogenesis 31(10):1762-1769(2010). Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010). Pace, P., et al. Science 329(5988):219-223(2010). Sobczuk, A., et al. Pol J Pathol 61(1):27-31(2010).

Other Notes

Small volumes of anti-XRCC6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for XRCC6 purchase

MBS6364126 is an antibody targeting XRCC6. Also known as XRCC6, ID (XRCC6, G22P1, X-ray repair cross-complementing protein 6, 5'-deoxyribose-5-phosphate lyase Ku70, 70kD subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70kD subunit, CTC box-binding factor 75kD subunit. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for XRCC6. It may not necessarily be applicable to this product.

NCBI GI #
4503841
NCBI GeneID
2547
NCBI Accession #
NP_001460.1
UniProt Primary Accession #
P12956
UniProt Secondary Accession #
Q6FG89; Q9UCQ2; Q9UCQ3
UniProt Related Accession #
P12956
GenBank Nucleotide #
NM_001469.3
NCBI Official Full Name
X-ray repair cross-complementing protein 6
NCBI Official Synonym Full Names
X-ray repair complementing defective repair in Chinese hamster cells 6
NCBI Official Symbol
XRCC6
NCBI Official Synonym Symbols
ML8; KU70; TLAA; CTC75; CTCBF; G22P1
UniProt Protein Name
X-ray repair cross-complementing protein 6
UniProt Synonym Protein Names
5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; Lupus Ku autoantigen protein p70; Ku70; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 6
Protein Family
X-ray repair cross-complementing protein
UniProt Gene Name
XRCC6
UniProt Synonym Gene Names
G22P1; 5'-dRP lyase Ku70; CTC75; CTCBF; Ku70; TLAA
UniProt Entry Name
XRCC6_HUMAN
NCBI Protein Information
X-ray repair cross-complementing protein 6; 5'-dRP lyase Ku70; Ku autoantigen, 70kDa; DNA repair protein XRCC6; Ku autoantigen p70 subunit; 70 kDa subunit of Ku antigen; thyroid-lupus autoantigen p70; lupus Ku autoantigen protein p70; 5'-deoxyribose-5-pho

UniProt Comments for XRCC6

Ku70: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.

Protein type: Helicase; EC 3.6.4.-; DNA-binding; DNA repair, damage; Nuclear receptor co-regulator; EC 4.2.99.-; RNA-binding

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: nucleoplasm; transcription factor complex; nuclear telomere cap complex; membrane; nucleolus; cytosol; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein C-terminus binding; protein binding; DNA binding; double-stranded DNA binding; damaged DNA binding; double-stranded telomeric DNA binding; 5'-deoxyribose-5-phosphate lyase activity; ATP binding

Biological Process: positive regulation of neurogenesis; V(D)J recombination; transcription, DNA-dependent; viral reproduction; positive regulation of transcription, DNA-dependent; DNA repair; double-strand break repair via nonhomologous end joining; DNA duplex unwinding; DNA ligation; double-strand break repair; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; brain development; negative regulation of transcription, DNA-dependent; telomere maintenance
Show more

Research Articles on XRCC6

1. XRCC6 C1310G polymorphism is associated with risk of cancer development.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-XRCC6 antibody

 Reference Product  PubMed Publications
 XRCC5 antibody  >14 publications with XRCC6 and XRCC5
 PRKDC antibody  >4 publications with XRCC6 and PRKDC
 PCNA antibody  >2 publications with XRCC6 and PCNA
 MRE11A antibody  >2 publications with XRCC6 and MRE11A
 PARP1 antibody  >1 publications with XRCC6 and PARP1
 CHEK1 antibody  >1 publications with XRCC6 and CHEK1
 CDKN1A antibody  >1 publications with XRCC6 and CDKN1A
 APEX1 antibody  >1 publications with XRCC6 and APEX1
 WRN antibody  >1 publications with XRCC6 and WRN
 TP53 antibody  >1 publications with XRCC6 and TP53

Pathways associated with anti-XRCC6 antibody

 Products by Pathway  Pathway Diagram
 2-LTR Circle Formation Pathway antibodies  2-LTR Circle Formation Pathway Diagram
 BARD1 Signaling Events Pathway antibodies  BARD1 Signaling Events Pathway Diagram
 Coregulation Of Androgen Receptor Activity Pathway antibodies  Coregulation Of Androgen Receptor Activity Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 DNA-PK Complex Pathway antibodies  DNA-PK Complex Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Double-Strand Break Repair Pathway antibodies  Double-Strand Break Repair Pathway Diagram
 Early Phase Of HIV Life Cycle Pathway antibodies  Early Phase Of HIV Life Cycle Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram

Diseases associated with anti-XRCC6 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >22 publications with XRCC6 and Neoplasms
 Carcinoma Antibodies  >10 publications with XRCC6 and Carcinoma
 Adenocarcinoma Antibodies  >8 publications with XRCC6 and Adenocarcinoma
 Carcinoma, Hepatocellular Antibodies  >4 publications with XRCC6 and Carcinoma, Hepatocellular
 Breast Neoplasms Antibodies  >4 publications with XRCC6 and Breast Neoplasms
 Lung Neoplasms Antibodies  >3 publications with XRCC6 and Lung Neoplasms
 Liver Diseases Antibodies  >3 publications with XRCC6 and Liver Diseases
 Lymphoma Antibodies  >3 publications with XRCC6 and Lymphoma
 Liver Neoplasms Antibodies  >3 publications with XRCC6 and Liver Neoplasms
 Leukemia Antibodies  >2 publications with XRCC6 and Leukemia

Organs/Tissues associated with anti-XRCC6 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5 publications with XRCC6 and Blood
 Liver Antibodies  >4 publications with XRCC6 and Liver
 Lung Antibodies  >3 publications with XRCC6 and Lung
 Kidney Antibodies  >2 publications with XRCC6 and Kidney
 Testis Antibodies  >2 publications with XRCC6 and Testis
 Brain Antibodies  >2 publications with XRCC6 and Brain
 Prostate Antibodies  >1 publications with XRCC6 and Prostate
 Connective Tissue Antibodies  >1 publications with XRCC6 and Connective Tissue
 Embryonic Tissue Antibodies  >1 publications with XRCC6 and Embryonic Tissue
 Skin Antibodies  >1 publications with XRCC6 and Skin
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000