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ZNF234 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8109401

ZNF234 (Zinc Finger Protein 234), also known as HZF4 or ZNF269, is located on chromosome 19q13.31. ZNF226 is an important paralog of ZNF234 gene. ZNF234 belongs to the krueppel C2H2-type zinc-finger protein family. Zinc finger proteins have been shown to… More details

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Product Overview

Online Datasheet in PDF
Product Name
ZNF234, Polyclonal Antibody
Full Product Name
Anti-ZNF234 Antibody, Rabbit Polyclonal
Product Gene Name
anti-ZNF234 antibody
Product Synonym Names
ZNF234 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-HZF4 Antibody; Anti-ZNF269 Antibody
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Purity/Purification
Protein A & Antigen Affinity
Specificity
Human ZNF234
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunogen
E Coli-derived Human ZNF234 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human ZNF234 fragment, and purified by antigen affinity chromatography.
Applications Tested/Suitable for anti-ZNF234 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes for anti-ZNF234 antibody
WB: 1:500-1:2000
IHC-P: 1:50-1:200
IP: 1-5uL/mg of lysate

Western Blot (WB) of anti-ZNF234 antibody

Anti-ZNF234 rabbit polyclonal antibody at 1:500 dilution
Lane A: HCT116 Whole Cell Lysate
Lane B: U-251 MG Whole Cell Lysate
Lysates/proteins at 30 ug per lane.
Secondary
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:81 kDa
Observed band size:81 kDa
Rabbit Anti-ZNF234 Polyclonal Antibody – Cat# MBS8109401

Immunohistochemistry (IHC) of anti-ZNF234 antibody

Immunochemical staining of human ZNF234 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Rabbit Anti-ZNF234 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS8109401

Immunohistochemistry (IHC) of anti-ZNF234 antibody

Immunochemical staining of human ZNF234 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Rabbit Anti-ZNF234 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS8109401

Immunoprecipitation (IP) of anti-ZNF234 antibody

ZNF234 was immunoprecipitated using:
Lane A:0.5 mg U-251 MG Whole Cell Lysate
4 uL anti-ZNF234 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.
Primary antibody:
Anti-ZNF234 rabbit polyclonal antibody,at 1:100 dilution
Secondary antibody:
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 81 kDa
Observed band size :81 kDa
Rabbit Anti-ZNF234 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS8109401

Product References and Citations for anti-ZNF234 antibody

Wang J, et al. (2008) Protein interaction data set highlighted with human ras-mapk/pi3k signaling pathways. J Proteome Res 7 (9): 3879-3889.Shannon M, et al. (2003) Differential expansion of zinc-finger transcription factor loci in homologous human and mouse gene clusters. Genome Res 13 (6a): 1097-1110.Abrink M, et al. (1995) Isolation of cdna clones for 42 different kruppel-related zinc finger proteins expressed in the human monoblast cell line u-937. DNA Cell Biol 14 (2): 125-136.

Other Notes

Small volumes of anti-ZNF234 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ZNF234 purchase

MBS8109401 is an antibody targeting ZNF234. Also known as Anti-ZNF234 Antibody, Rabbit Polyclonal. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ZNF234. It may not necessarily be applicable to this product.

Protein Family
Zinc finger protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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