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ZNF521 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS150459

ZNF521 Antibody: The zinc finger protein 521 (ZNF521) is a transcription factor containing an N-terminal transcriptional repressor motif and 30 zinc finger domains. It plays a role in both erythroid cell and osteoblast differentiation during development,… More details

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Product Overview

Online Datasheet in PDF
Product Name
ZNF521, Polyclonal Antibody
Full Product Name
ZNF521 Antibody
Product Gene Name
anti-ZNF521 antibody
Product Synonym Names
ZNF521; EHZF; Evi3; EHZF; LIP3; Zinc finger protein 521; Early hematopoietic zinc finger protein; zinc finger protein 521
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
ZNF521 Antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
ZNF521 antibody is human specific.
Preparation and Storage
ZNF521 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
ZNF521 peptide (MBS153010) is used for blocking the activity of ZNF521 antibody (MBS150459)
Molecular Weight
147,866 Da
OMIM
610974
3D Structure
ModBase 3D Structure for Q96K83
Conjugate
Unconjugated
Immunogen
ZNF521 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ZNF521.
Buffer
ZNF521 Antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-ZNF521 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-ZNF521 antibody
ZNF521 antibody can be used for detection of ZNF521 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 2.5 mug/mL. For immunofluorescence start at 2.5 mug/mL.

Western Blot (WB) of anti-ZNF521 antibody

Western blot analysis of ZNF521 in HeLa cell lysate with ZNF521 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Rabbit Anti-ZNF521 Polyclonal Antibody – Cat# MBS150459

Immunocytochemistry (IHC) of anti-ZNF521 antibody

Immunocytochemistry of ZNF521 in HeLa cells with ZNF521 antibody at 2.5 μg/mL.
Rabbit Anti-ZNF521 Polyclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS150459

Immunofluorescence (IF) of anti-ZNF521 antibody

Immunofluorescence of ZNF521 in HeLa cells with ZNF521 antibody at 20 μg/mL.
Rabbit Anti-ZNF521 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS150459

Other Notes

Small volumes of anti-ZNF521 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ZNF521 purchase

MBS150459 is an antibody targeting ZNF521. Also known as ZNF521 Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ZNF521. It may not necessarily be applicable to this product.

NCBI GI #
24308069
NCBI GeneID
25925
NCBI Accession #
NP_056276
UniProt Primary Accession #
Q96K83
UniProt Secondary Accession #
Q8IXI0; Q8TES6; Q9C065; Q9HAL5; Q9UFK4; A3QVP7; B0YJB7
UniProt Related Accession #
Q96K83
GenBank Nucleotide #
NM_015461.2
NCBI Official Full Name
zinc finger protein 521
NCBI Official Synonym Full Names
zinc finger protein 521
NCBI Official Symbol
ZNF521
NCBI Official Synonym Symbols
EHZF; Evi3
UniProt Protein Name
Zinc finger protein 521
UniProt Synonym Protein Names
Early hematopoietic zinc finger protein; LYST-interacting protein 3
Protein Family
Zinc finger protein
UniProt Gene Name
ZNF521
UniProt Synonym Gene Names
EHZF; LIP3
UniProt Entry Name
ZN521_HUMAN
NCBI Protein Information
zinc finger protein 521; LYST-interacting protein 3; early hematopoietic zinc finger protein

UniProt Comments for ZNF521

ZNF521: Transcription factor that can both act as an activator or a repressor depending on the context. Involved in BMP signaling and in the regulation of the immature compartment of the hematopoietic system. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved specification of B-cell lineage; this interaction preventing EBF1 to bind DNA and activate target genes. A chromosomal aberration involving ZNF521 is found in acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with PAX5. The translocation generates the PAX5-ZNF521 oncogene consisting of the N-terminus part of PAX5 and the C-terminus part of ZNF521. Belongs to the krueppel C2H2-type zinc-finger protein family.

Protein type: Oncoprotein; Transcription, coactivator/corepressor; C2H2-type zinc finger protein; DNA-binding

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: nucleus

Molecular Function: protein domain specific binding; DNA binding; metal ion binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; multicellular organismal development; cell differentiation
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Research Articles on ZNF521

1. Data strongly support the notion that ZNF521, through the recruitment of the NuRD complex, contributes to the clonogenic growth, migration and tumorigenicity of medulloblastoma cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ZNF521 antibody

 Reference Product  PubMed Publications
 EBF1 antibody  >3 publications with ZNF521 and EBF1
 BMP2 antibody  >1 publications with ZNF521 and BMP2
 GATA1 antibody  >1 publications with ZNF521 and GATA1

Diseases associated with anti-ZNF521 antibody

 Disease Name  Pubmed Publications
 Multiple Myeloma Antibodies  >1 publications with ZNF521 and Multiple Myeloma
 Prenatal Injuries Antibodies  >1 publications with ZNF521 and Prenatal Injuries

Organs/Tissues associated with anti-ZNF521 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >2 publications with ZNF521 and Brain
 Bone Antibodies  >1 publications with ZNF521 and Bone
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