Rabbit Anti-TRIM63 Polyclonal Antibody – Cat# MBS1753484

Figure 1. Western blot analysis of MURF1/TRIM63 using anti-MURF1/TRIM63 antibody (MBS1753484).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: monkey heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-MURF1/TRIM63 antigen affinity purified polyclonal antibody (Catalog # MBS1753484) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for MURF1/TRIM63 at approximately 40KD. The expected band size for MURF1/TRIM63 is at 40KD.

MURF1/TRIM63 (TRIM63) Antibody – Rabbit Polyclonal

Name: TRIM63 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1753484
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1753484

E3 ubiquitin-protein ligase TRIM63 is an enzyme that in humans is encoded by the TRIM63 gene. This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that… More details

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Technical Datasheet View PDF

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Product Overview

Online Datasheet in PDF

Product Name

MURF1/TRIM63, Polyclonal Antibody

Full Product Name

Anti-MURF1/TRIM63 Antibody

Product Gene Name

anti-TRIM63 antibody

Product Synonym Names

TRIM63; IRF; MURF1; RNF28; SMRZ; E3 ubiquitin-protein ligase TRIM63; EC 2. 3. 2. 27; Iris RING finger protein; Muscle-specific RING finger protein 1; MuRF-1; MuRF1; RING finger protein 28; RING-type E3 ubiquitin transferase TRIM63; Striated muscle RING zinc finger protein; Tripartite motif-containing protein 63; tripartite motif containing 63

Clonality

Polyclonal

Isotype

Rabbit IgG

Host

Rabbit

Species Reactivity

Human, Monkey

Form/Format

Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purity/Purification

Immunogen affinity purified.

Specificity

Rabbit IgG polyclonal antibody for MURF1/TRIM63 detection.

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

353

OMIM

606131

3D Structure

ModBase 3D Structure for Q969Q1

Immunogen

E Coli-derived human MURF1/TRIM63 recombinant protein (Position: D2-E329).

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Recommended Detection Systems

Recommended Detection Systems||Lab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-TRIM63 antibody

Western Blot (WB), Direct ELISA (EIA)

Application Notes for anti-TRIM63 antibody

WB: 0.25-0.5ug/ml|Monkey|
Direct ELISA: 0.1-0.5ug/ml|Human|

Product References and Citations for anti-TRIM63 antibody

1. Bodine, S. C., Latres, E., Baumhueter, S., Lai, V. K. -M., Nunez, L., Clarke, B. A., Poueymirou, W. T., Panaro, F. J., Na, E., Dharmarajan, K., Pan, Z. -Q., Valenzuela, D. M., DeChiara, T. M., Stitt, T. N., Yancopoulos, G. D., Glass, D. J. Identification of ubiquitin ligases required for skeletal muscle atrophy. Science 294: 1704-1708, 2001.
2. Cai, D., Frantz, J. D., Tawa, N. E., Jr., Melendez, P. A., Oh, B. -C., Lidov, H. G. W., Hasselgren, P. -O., Frontera, W. R., Lee, J., Glass, D. J., Shoelson, S. E. IKK-beta/NF-kappa-B activation causes severe muscle wasting in mice. Cell 119: 285-298, 2004.
3. Centner, T., Yano, J., Kimura, E., McElhinny, A. S., Pelin, K., Witt, C. C., Bang, M. -L., Trombitas, K., Granzier, H., Gregorio, C. C., Sorimachi, H., Labeit, S. Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain. J. Molec. Biol. 306: 717-726, 2001.

Other Notes

Small volumes of anti-TRIM63 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TRIM63 purchase

MBS1753484 is an antibody targeting TRIM63. Also known as Anti-MURF1/TRIM63 Antibody. Reactive in Human, Monkey. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TRIM63. It may not necessarily be applicable to this product.

NCBI GI #

21362898

NCBI GeneID

84676

NCBI Accession #

Q969Q1.1

UniProt Primary Accession #

Q969Q1

UniProt Secondary Accession #

Q5T2I1; Q96BD3; Q96KD9; Q9BYV4; B4DN95

UniProt Related Accession #

Q969Q1

NCBI Official Full Name

E3 ubiquitin-protein ligase TRIM63

NCBI Official Synonym Full Names

tripartite motif containing 63, E3 ubiquitin protein ligase

NCBI Official Symbol

TRIM63

NCBI Official Synonym Symbols

IRF; SMRZ; MURF1; MURF2; RNF28

UniProt Protein Name

E3 ubiquitin-protein ligase TRIM63

UniProt Synonym Protein Names

Iris RING finger protein; Muscle-specific RING finger protein 1; MuRF-1; MuRF1; RING finger protein 28; Striated muscle RING zinc finger protein; Tripartite motif-containing protein 63

Protein Family

E3 ubiquitin-protein ligase

UniProt Gene Name

TRIM63

UniProt Synonym Gene Names

IRF; MURF1; RNF28; SMRZ; MuRF-1; MuRF1

UniProt Entry Name

TRI63_HUMAN

NCBI Protein Information

E3 ubiquitin-protein ligase TRIM63; ring finger protein 28; iris ring finger protein; muscle specific ring finger protein 2; muscle-specific RING finger protein 1; tripartite motif-containing protein 63; striated muscle RING zinc finger protein

NCBI Summary for TRIM63

This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase. [provided by RefSeq, Feb 2012]

UniProt Comments for TRIM63

MURF1: E3 ubiquitin ligase. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric- associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.

Protein type: EC 6.3.2.-; Ligase; EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1p34-p33

Cellular Component: microtubule; cytoplasm; M band; Z disc; nucleus

Molecular Function: signal transducer activity; protein binding; zinc ion binding; ubiquitin-protein ligase activity; titin binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; skeletal muscle atrophy; muscle contraction; regulation of gene expression; protein ubiquitination; response to electrical stimulus involved in regulation of muscle adaptation; signal transduction

Research Articles on TRIM63

1. Data reveal that Titin protein is a pseudokinase with non-detectable catalytic output but is a high-affinity binding locus for MuRF1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TRIM63 antibody

Reference Product	PubMed Publications
TTN antibody	>6 publications with TRIM63 and TTN
SQSTM1 antibody	>3 publications with TRIM63 and SQSTM1
PKM2 antibody	>1 publications with TRIM63 and PKM2
MYOZ1 antibody	>1 publications with TRIM63 and MYOZ1
SUMO2 antibody	>1 publications with TRIM63 and SUMO2

Pathways associated with anti-TRIM63 antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies	Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies	Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
TWEAK Signaling Pathway antibodies	TWEAK Signaling Pathway Diagram

Diseases associated with anti-TRIM63 antibody

Disease Name	Pubmed Publications
Atrophy Antibodies	>237 publications with TRIM63 and Atrophy
Nervous System Diseases Antibodies	>170 publications with TRIM63 and Nervous System Diseases
Muscular Atrophy Antibodies	>161 publications with TRIM63 and Muscular Atrophy
Hypertrophy Antibodies	>57 publications with TRIM63 and Hypertrophy
Disease Models, Animal Antibodies	>53 publications with TRIM63 and Disease Models, Animal
Weight Loss Antibodies	>43 publications with TRIM63 and Weight Loss
Necrosis Antibodies	>36 publications with TRIM63 and Necrosis
Cardiovascular Diseases Antibodies	>35 publications with TRIM63 and Cardiovascular Diseases
Inflammation Antibodies	>34 publications with TRIM63 and Inflammation
Heart Diseases Antibodies	>33 publications with TRIM63 and Heart Diseases

Organs/Tissues associated with anti-TRIM63 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>338 publications with TRIM63 and Muscle
Heart Antibodies	>59 publications with TRIM63 and Heart
Vascular Antibodies	>13 publications with TRIM63 and Vascular
Lung Antibodies	>13 publications with TRIM63 and Lung
Liver Antibodies	>12 publications with TRIM63 and Liver
Embryonic Tissue Antibodies	>9 publications with TRIM63 and Embryonic Tissue
Brain Antibodies	>5 publications with TRIM63 and Brain
Skin Antibodies	>2 publications with TRIM63 and Skin
Uncharacterized Tissue Antibodies	>1 publications with TRIM63 and Uncharacterized Tissue