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NCL Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9201499

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, isinvolved in the synthesis and maturation of ribosomes. It islocated mainly in dense fibrillar regions of the nucleolus. HumanNCL gene consists of 14 exons with 13 introns and spansapproximately 11kb.… More details

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Product Overview

Online Datasheet in PDF
Product Name
NCL, Polyclonal Antibody
Full Product Name
NCL Antibody (Center E443)
Product Gene Name
anti-NCL antibody
Product Synonym Names
Nucleolin; Protein C23; NCL
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human (Predicted Reactivity: Monkey)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Concentration
Vial Concentration: 0.31 (lot specific)
Specificity
This NCL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 428-455 amino acids from the Central region of human NCL.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
NCL peptide (MBS9218556) is used for blocking the activity of NCL antibody (MBS9201499)
Molecular Weight
76614
Immunogen Sequence Positions
428-455
OMIM
164035
3D Structure
ModBase 3D Structure for P19338
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Applications Tested/Suitable for anti-NCL antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-NCL antibody
WB~~1:1000

Western Blot (WB) of anti-NCL antibody

NCL Antibody (Center E443) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NCL antibody detected the NCL protein (arrow).
Rabbit Anti-NCL Polyclonal Antibody – Cat# MBS9201499

Western Blot (WB) of anti-NCL antibody

Western blot analysis of NCL (arrow) using rabbit polyclonal NCL Antibody (Center E443). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCL gene.
Rabbit Anti-NCL Polyclonal Antibody, Western Blot (WB) – Cat# MBS9201499

Immunohistochemistry (IHC) of anti-NCL antibody

NCL Antibody (Center E443) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NCL Antibody (Center E443) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-NCL Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9201499

Immunofluorescence (IF) of anti-NCL antibody

Fluorescent confocal image of Hela cell stained with NCL Antibody (Center E443). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NCL primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NCL immunoreactivity is localized to nucleus significantly.
Rabbit Anti-NCL Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9201499

Product References and Citations for anti-NCL antibody

Ishimaru, D., et al. J. Biol. Chem. 285(35):27182-27191(2010)
Tulchin, N., et al. Am. J. Pathol. 176(3):1203-1214(2010)
Strang, B.L., et al. J. Virol. 84(4):1771-1784(2010)
Bertrand, L., et al. J. Virol. 84(1):109-118(2010)
Jerke, U., et al. PLoS ONE 4 (12), E8302 (2009) :

Other Notes

Small volumes of anti-NCL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NCL purchase

MBS9201499 is an antibody targeting NCL. Also known as NCL Antibody (Center E443). Reactive in Human (Predicted Reactivity: Monkey). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NCL. It may not necessarily be applicable to this product.

NCBI GI #
55956788
NCBI GeneID
4691
NCBI Accession #
NP_005372.2
UniProt Primary Accession #
P19338
UniProt Secondary Accession #
Q53SK1; Q8NB06; Q9UCF0; Q9UDG1
UniProt Related Accession #
P19338
GenBank Nucleotide #
NM_005381.2
NCBI Official Full Name
nucleolin
NCBI Official Synonym Full Names
nucleolin
NCBI Official Symbol
NCL
NCBI Official Synonym Symbols
C23
UniProt Protein Name
Nucleolin
UniProt Synonym Protein Names
Protein C23
Protein Family
Nucleolin
UniProt Gene Name
NCL
UniProt Entry Name
NUCL_HUMAN
NCBI Protein Information
nucleolin

NCBI Summary for NCL

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]
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UniProt Comments for NCL

NCL: the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and preribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-RNA transcription and ribosome assembly.

Protein type: Helicase; Nucleolus; DNA-binding; RNA-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: nucleoplasm; membrane; nucleolus; cell cortex; ribonucleoprotein complex; nucleus

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; RNA binding; nucleotide binding; telomeric DNA binding

Biological Process: angiogenesis
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Research Articles on NCL

1. Data show that nucleolin and endogenous galectin-3 exist in the same complexes in the nucleolus, the cytoplasm, and on the cell surface of melanoma cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NCL antibody

 Reference Product  PubMed Publications
 MYC antibody  >12 publications with NCL and MYC
 MDM2 antibody  >10 publications with NCL and MDM2
 TP53 antibody  >7 publications with NCL and TP53
 NPM1 antibody  >3 publications with NCL and NPM1

Diseases associated with anti-NCL antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1306 publications with NCL and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >381 publications with NCL and Cardiovascular Diseases
 Heart Diseases Antibodies  >169 publications with NCL and Heart Diseases
 Neurobehavioral Manifestations Antibodies  >165 publications with NCL and Neurobehavioral Manifestations
 Disease Models, Animal Antibodies  >165 publications with NCL and Disease Models, Animal
 Liver Diseases Antibodies  >148 publications with NCL and Liver Diseases
 Atrophy Antibodies  >136 publications with NCL and Atrophy
 Pain Antibodies  >130 publications with NCL and Pain
 Seizures Antibodies  >111 publications with NCL and Seizures
 Kidney Diseases Antibodies  >105 publications with NCL and Kidney Diseases

Organs/Tissues associated with anti-NCL antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >845 publications with NCL and Brain
 Blood Antibodies  >707 publications with NCL and Blood
 Muscle Antibodies  >256 publications with NCL and Muscle
 Heart Antibodies  >237 publications with NCL and Heart
 Liver Antibodies  >222 publications with NCL and Liver
 Vascular Antibodies  >216 publications with NCL and Vascular
 Skin Antibodies  >194 publications with NCL and Skin
 Nerve Antibodies  >192 publications with NCL and Nerve
 Lung Antibodies  >163 publications with NCL and Lung
 Bone Antibodies  >158 publications with NCL and Bone
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