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PTGER4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247972

Prostaglandin E receptor EP4 is a Prostanoid Receptor with a relaxing effect on smooth muscle. It may play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function. More details

$630 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PTGER4 / EP4, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human PTGER4 / EP4
Product Gene Name
anti-PTGER4 antibody
Product Synonym Gene Name
EP4
Product Synonym Names
Anti-PTGER4 / EP4 Antibody (Extracellular Domain) IHC-plus; PTGER4; EP4; EP4 prostaglandin receptor; EP4R; PGE receptor EP4 subtype; Prostanoid EP4 receptor; Prostaglandin E receptor 4; PGE receptor; EP4 subtype; PGE2 receptor EP4 subtype; Human PTGER4; EP4
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Monkey
Form/Format
PBS, 0.1% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
0.9 mg/ml (lot specific)
Specificity
Human PTGER4 / EP4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Preparation and Storage
Aliquot and store undiluted at -20°C or below for up to a 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles
OMIM
601586
3D Structure
ModBase 3D Structure for P35408
Target Species
Human
Epitope
Extracellular Domain
Immunogen Type
Synthetic peptide
Immunogen
PTGER4 / EP4 antibody was raised against synthetic 17 amino acid peptide from 3rd extracellular domain of human PTGER4 / EP4. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%); Guinea pig (88%); Elephant, Bat, Rabbit, Horse (82%).
Immunogen Species
PTGER4 / EP4 antibody was raised against human
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-PTGER4 antibody

Family: GPCR; Subfamily: Prostanoid
Applications Tested/Suitable for anti-PTGER4 antibody
Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC)
Application Notes for anti-PTGER4 antibody
ICC, IHC-P (9 - 18 ug/ml)

(Optimal dilution to be determined by the researcher)

Immunohistochemistry (IHC) of anti-PTGER4 antibody

Human, Liver: Formalin-Fixed Paraffin-Embedded (FFPE)
Rabbit Anti-PTGER4 Polyclonal Antibody – Cat# MBS247972

Transfection of anti-PTGER4 antibody

 
Rabbit Anti-PTGER4 Polyclonal Antibody, Transfection – Cat# MBS247972

Transfection Control of anti-PTGER4 antibody

 
Rabbit Anti-PTGER4 Polyclonal Antibody, Transfection Control – Cat# MBS247972

Other Notes

Small volumes of anti-PTGER4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PTGER4 purchase

MBS247972 is an antibody targeting PTGER4. Also known as Rabbit Polyclonal to Human PTGER4 / EP4. Reactive in Human, Monkey. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PTGER4. It may not necessarily be applicable to this product.

NCBI GI #
4506259
NCBI GeneID
5734
NCBI Accession #
NP_000949.1
UniProt Primary Accession #
P35408
UniProt Secondary Accession #
Q3MJ87
UniProt Related Accession #
P35408
GenBank Nucleotide #
NM_000958.2
NCBI Official Full Name
prostaglandin E2 receptor EP4 subtype
NCBI Official Synonym Full Names
prostaglandin E receptor 4 (subtype EP4)
NCBI Official Symbol
PTGER4
NCBI Official Synonym Symbols
EP4; EP4R
UniProt Protein Name
Prostaglandin E2 receptor EP4 subtype
UniProt Synonym Protein Names
Prostanoid EP4 receptor
Protein Family
Prostaglandin E2 receptor
UniProt Gene Name
PTGER4
UniProt Synonym Gene Names
PTGER2; PGE receptor EP4 subtype; PGE2 receptor EP4 subtype
UniProt Entry Name
PE2R4_HUMAN
NCBI Protein Information
prostaglandin E2 receptor EP4 subtype; prostanoid EP4 receptor; PGE receptor EP4 subtype; PGE receptor, EP4 subtype; PGE2 receptor EP4 subtype

NCBI Summary for PTGER4

The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor can activate T-cell factor signaling. It has been shown to mediate PGE2 induced expression of early growth response 1 (EGR1), regulate the level and stability of cyclooxygenase-2 mRNA, and lead to the phosphorylation of glycogen synthase kinase-3. Knockout studies in mice suggest that this receptor may be involved in the neonatal adaptation of circulatory system, osteoporosis, as well as initiation of skin immune responses. [provided by RefSeq, Jul 2008]
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UniProt Comments for PTGER4

PTGER4: Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p13.1

Cellular Component: integral to membrane; plasma membrane

Molecular Function: protein binding; prostaglandin E receptor activity

Biological Process: T-helper cell differentiation; negative regulation of cytokine secretion; response to lipopolysaccharide; negative regulation of integrin activation; G-protein signaling, coupled to cAMP nucleotide second messenger; response to mechanical stimulus; negative regulation of inflammatory response; regulation of stress fiber formation; JNK cascade; immune response; regulation of ossification; positive regulation of inflammatory response; positive regulation of cytokine secretion
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Research Articles on PTGER4

1. potential relevance of the COX-2/mPGES-1/EP-4 axis in the AAA-associated hypervascularization

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PTGER4 antibody

 Reference Product  PubMed Publications
 PTGER2 antibody  >208 publications with PTGER4 and PTGER2
 PTGS2 antibody  >147 publications with PTGER4 and PTGS2
 PTH antibody  >7 publications with PTGER4 and PTH
 PTGIR antibody  >5 publications with PTGER4 and PTGIR
 POMC antibody  >1 publications with PTGER4 and POMC

Pathways associated with anti-PTGER4 antibody

 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Eicosanoid Ligand-binding Receptors Pathway antibodies  Eicosanoid Ligand-binding Receptors Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Inflammatory Mediator Regulation Of TRP Channels Pathway antibodies  Inflammatory Mediator Regulation Of TRP Channels Pathway Diagram
 Inflammatory Mediator Regulation Of TRP Channels Pathway antibodies  Inflammatory Mediator Regulation Of TRP Channels Pathway Diagram

Diseases associated with anti-PTGER4 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >83 publications with PTGER4 and Inflammation
 Disease Models, Animal Antibodies  >59 publications with PTGER4 and Disease Models, Animal
 Necrosis Antibodies  >48 publications with PTGER4 and Necrosis
 Cardiovascular Diseases Antibodies  >42 publications with PTGER4 and Cardiovascular Diseases
 Carcinoma Antibodies  >33 publications with PTGER4 and Carcinoma
 Nervous System Diseases Antibodies  >32 publications with PTGER4 and Nervous System Diseases
 Heart Diseases Antibodies  >15 publications with PTGER4 and Heart Diseases
 Kidney Diseases Antibodies  >14 publications with PTGER4 and Kidney Diseases
 Neoplasms, Experimental Antibodies  >14 publications with PTGER4 and Neoplasms, Experimental
 Breast Neoplasms Antibodies  >14 publications with PTGER4 and Breast Neoplasms

Organs/Tissues associated with anti-PTGER4 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >127 publications with PTGER4 and Blood
 Vascular Antibodies  >62 publications with PTGER4 and Vascular
 Kidney Antibodies  >41 publications with PTGER4 and Kidney
 Brain Antibodies  >40 publications with PTGER4 and Brain
 Intestine Antibodies  >30 publications with PTGER4 and Intestine
 Lung Antibodies  >30 publications with PTGER4 and Lung
 Eye Antibodies  >22 publications with PTGER4 and Eye
 Connective Tissue Antibodies  >19 publications with PTGER4 and Connective Tissue
 Liver Antibodies  >19 publications with PTGER4 and Liver
 Skin Antibodies  >16 publications with PTGER4 and Skin
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