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RUFY4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1759488

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
RUFY4, Polyclonal Antibody
Full Product Name
Anti-RUFY4 Antibody Picoband
Product Gene Name
anti-RUFY4 antibody
Product Synonym Names
Transmembrane protein 240; kelch repeat and BTB domain containing 2; Kelch repeat and BTB domain-containing protein 2; BTB and kelch domain-containing protein 1; KBTBD2; BKLHD1; KIAA1489
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Monkey
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
64,350 Da
3D Structure
ModBase 3D Structure for Q6ZNE9
Immunogen
E coli-derived human RUFY4 recombinant protein (Position: M1-K553).
Subcellular Localization
Cul3-RING ubiquitin ligase complex
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.

Product Categories/Family for anti-RUFY4 antibody

Primary Antibodies; Cell Biology
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-RUFY4 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-RUFY4 antibody
WB: 0.25-0.5ug/ml, Human, Monkey
IHC(Paraffin-embedded Section): 2-5ug/ml, Human
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-RUFY4 antibody

Figure 1. Western blot analysis of RUFY4 using anti-RUFY4 antibody (MBS1759488).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: monkey COS-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUFY4 antigen affinity purified polyclonal antibody (#MBS1759488) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for RUFY4 at approximately 64 kDa. The expected band size for RUFY4 is at 64 kDa.
Rabbit Anti-RUFY4 Polyclonal Antibody – Cat# MBS1759488

Immunohistochemistry (IHC) of anti-RUFY4 antibody

Figure 2. IHC analysis of RUFY4 using anti-RUFY4 antibody (MBS1759488).
RUFY4 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RUFY4 Antibody (MBS1759488) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (#MBS176453) with DAB as the chromogen.
Rabbit Anti-RUFY4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1759488

Flow Cytometry (FC/FACS) of anti-RUFY4 antibody

Figure 3. Flow Cytometry analysis of HEL cells using anti-RUFY4 antibody (MBS1759488).
Overlay histogram showing HEL cells stained with MBS1759488 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RUFY4 Antibody (MBS1759488, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Rabbit Anti-RUFY4 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1759488

Flow Cytometry (FC/FACS) of anti-RUFY4 antibody

Figure 4. Flow Cytometry analysis of THP-1 cells using anti-RUFY4 antibody (MBS1759488).
Overlay histogram showing THP-1 cells stained with MBS1759488 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RUFY4 Antibody (MBS1759488, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Rabbit Anti-RUFY4 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1759488

Product References and Citations for anti-RUFY4 antibody

1. Terawaki, S. , Camosseto, V. , Prete, F. , Wenger, T. , Papadopoulos, A. , & Rondeau, C. , et al. (2015). Run and fyve domain-containing protein 4 enhances autophagy and lysosome tethering in response to interleukin-4. The Journal of Cell Biology, 210(7), 1133-1152.2. Chen, S. , Robert, F. , Lambertus, K. , Yuan, L. , Wang, J. , & Kathryn, R. , et al. (2018). An interactome perturbation framework prioritizes damaging missense mutations for developmental disorders. Nature Genetics.3. Haenig, C. , Atias, N. , Taylor, A. K. , Mazza, A. , & Wanker, E. E. . (2020). Interactome mapping provides a network of neurodegenerative disease proteins and uncovers widespread protein aggregation in affected brains. Cell Reports, 32(7), 108050.

Other Notes

Small volumes of anti-RUFY4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RUFY4 purchase

MBS1759488 is an antibody targeting RUFY4. Also known as Anti-RUFY4 Antibody Picoband. Reactive in Human, Monkey. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RUFY4. It may not necessarily be applicable to this product.

NCBI GI #
282165733
NCBI GeneID
285180
NCBI Accession #
NP_940885.2
UniProt Primary Accession #
Q6ZNE9
UniProt Secondary Accession #
Q6ZR96
UniProt Related Accession #
Q6ZNE9
GenBank Nucleotide #
NM_198483.3
NCBI Official Full Name
RUN and FYVE domain-containing protein 4
NCBI Official Synonym Full Names
RUN and FYVE domain containing 4
NCBI Official Symbol
RUFY4
UniProt Protein Name
RUN and FYVE domain-containing protein 4
Protein Family
RUN and FYVE domain-containing protein
UniProt Gene Name
RUFY4
UniProt Entry Name
RUFY4_HUMAN
NCBI Protein Information
RUN and FYVE domain-containing protein 4

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-RUFY4 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1 publications with RUFY4 and Muscle
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