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SUSD6 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1759404

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SUSD6, Polyclonal Antibody
Full Product Name
Anti-SUSD6 Antibody Picoband
Product Gene Name
anti-SUSD6 antibody
Product Synonym Names
DAP3 binding cell death enhancer 1; DAP3-binding cell death enhancer 1; Death ligand signal enhancer; DELE1; DELE; KIAA0141
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Monkey
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
32,090 Da
Chromosome Location
Chromosome: 14; NC_000014.8 (70078310..70181861). Location: 14q24.1
3D Structure
ModBase 3D Structure for Q92537
Immunogen
E coli-derived human SUSD6 recombinant protein (Position: L44-A303).
Subcellular Localization
Mitochondrion.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Essential for the induction of death receptor-mediated apoptosis through the regulation of caspase activation.

Product Categories/Family for anti-SUSD6 antibody

Primary Antibodies; Signal Transduction
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-SUSD6 antibody
Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-SUSD6 antibody
WB: 0.25-0.5ug/ml, Human, Monkey
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-SUSD6 antibody

Figure 1. Western blot analysis of SUSD6 using anti-SUSD6 antibody (MBS1759404).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Daudi whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: monkey COS-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUSD6 antigen affinity purified polyclonal antibody (#MBS1759404) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for SUSD6 at approximately 35 kDa. The expected band size for SUSD6 is at 32 kDa.
Rabbit Anti-SUSD6 Polyclonal Antibody – Cat# MBS1759404

Flow Cytometry (FC/FACS) of anti-SUSD6 antibody

Figure 2. Flow Cytometry analysis of Jurkat cells using anti-SUSD6 antibody (MBS1759404).
Overlay histogram showing Jurkat cells stained with MBS1759404 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUSD6 Antibody (MBS1759404, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Rabbit Anti-SUSD6 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1759404

Product References and Citations for anti-SUSD6 antibody

1. Hartz, P. A. Personal Communication. Baltimore, Md. 1/15/2016.2. Nagase, T., Seki, N., Ishikawa, K., Ohira, M., Kawarabayasi, Y., Ohara, O., Tanaka, A., Kotani, H., Miyajima, N., Nomura, N. Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain. DNA Res. 3: 321-329, 1996. 3. Polato, F., Rusconi, P., Zangrossi, S., Morelli, F., Boeri, M., Musi, A., Marchini, S., Castiglioni, V., Scanziani, E., Torri, V., Broggini, M. DRAGO (KIAA0247), a new DNA damage-responsive, p53-inducible gene that cooperates with p53 as oncosuppressor. J. Nat. Cancer Inst. 106: dju053, 2014. Note: Electronic Article. Erratum: J. Nat. Cancer Inst. 106: dju199, 2014.

Other Notes

Small volumes of anti-SUSD6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SUSD6 purchase

MBS1759404 is an antibody targeting SUSD6. Also known as Anti-SUSD6 Antibody Picoband. Reactive in Human, Monkey. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SUSD6. It may not necessarily be applicable to this product.

NCBI GI #
119601407
NCBI GeneID
9766
NCBI Accession #
EAW81001.1
UniProt Primary Accession #
Q92537
UniProt Related Accession #
Q92537
NCBI Official Full Name
KIAA0247
NCBI Official Synonym Full Names
KIAA0247
NCBI Official Symbol
KIAA0247
UniProt Protein Name
Uncharacterized protein KIAA0247
Protein Family
Sushi domain-containing protein
UniProt Gene Name
KIAA0247
UniProt Entry Name
K0247_HUMAN
NCBI Protein Information
uncharacterized protein KIAA0247

UniProt Comments for SUSD6

Subcellular location: Membrane; Single-pass type I membrane protein

Potential.

Sequence similarities: Contains 1 Sushi (CCP/SCR) domain.

Sequence caution: The sequence BAA13378.2 differs from that shown. Reason: Erroneous initiation.

Research Articles on SUSD6

1. This is the first description of a linkage between KIAA0247 and colorectal cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-SUSD6 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2 publications with SUSD6 and Inflammation

Organs/Tissues associated with anti-SUSD6 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2 publications with SUSD6 and Blood
 Nerve Antibodies  >1 publications with SUSD6 and Nerve
 Intestine Antibodies  >1 publications with SUSD6 and Intestine
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