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HEXIM1 Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8243164

MBS8243164 is an antibody targeting HEXIM1. Reactive in Human, Mouse, Rat, Monkey. Rabbit polyclonal antibody to HEXIM1 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
HEXIM1, Polyclonal Antibody
Full Product Name
Anti-HEXIM1 Antibody
Product Gene Name
anti-HEXIM1 antibody
Product Synonym Names
CLP1; EDG1; HIS1; MAQ1; Protein HEXIM1; Cardiac lineage protein 1; Estrogen down-regulated gene 1 protein; Hexamethylene bis-acetamide-inducible protein 1; Menage a quatre protein 1
Clonality
Polyclonal
Species Reactivity
Human, Mouse, Rat, Monkey
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of HEXIM1 protein.
Preparation and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
HEXIM1 peptide (MBS8243682) is used for blocking the activity of HEXIM1 antibody (MBS8243164)
Molecular Weight
40,623 Da
OMIM
607328
3D Structure
ModBase 3D Structure for O94992
Source
Rabbit
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HEXIM1. The exact sequence is proprietary.
Applications Tested/Suitable for anti-HEXIM1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-HEXIM1 antibody
Western Blot: (1/500 - 1/1000)
Immunohistochemistry: (1/100 - 1/200)
Immunofluorescence/Immunocytochemistry: (1/100 - 1/500)

Western Blot (WB) of anti-HEXIM1 antibody

Western blot analysis of HEXIM1 expression in A431 (A), Jurkat (B), NIH3T3 (C), rat heart (D) whole cell lysates.
Anti-HEXIM1 Polyclonal Antibody – Cat# MBS8243164

Immunohistochemistry (IHC) of anti-HEXIM1 antibody

Immunohistochemical analysis of HEXIM1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Anti-HEXIM1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS8243164

Immunofluorescence (IF) of anti-HEXIM1 antibody

Immunofluorescent analysis of HEXIM1 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Anti-HEXIM1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS8243164

Other Notes

Small volumes of anti-HEXIM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HEXIM1. It may not necessarily be applicable to this product.

NCBI GI #
74735573
NCBI GeneID
10614
NCBI Accession #
O94992.1
UniProt Primary Accession #
O94992
UniProt Secondary Accession #
B2R8Y5
UniProt Related Accession #
O94992
NCBI Official Full Name
Protein HEXIM1
NCBI Official Synonym Full Names
hexamethylene bis-acetamide inducible 1
NCBI Official Symbol
HEXIM1
NCBI Official Synonym Symbols
CLP1; EDG1; HIS1; MAQ1
UniProt Protein Name
Protein HEXIM1
UniProt Synonym Protein Names
Cardiac lineage protein 1; Estrogen down-regulated gene 1 protein; Hexamethylene bis-acetamide-inducible protein 1; Menage a quatre protein 1
Protein Family
Protein
UniProt Gene Name
HEXIM1
UniProt Synonym Gene Names
CLP1; EDG1; HIS1; MAQ1
UniProt Entry Name
HEXI1_HUMAN
NCBI Protein Information
protein HEXIM1

NCBI Summary for HEXIM1

Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns. [provided by RefSeq, Jul 2008]

UniProt Comments for HEXIM1

HEXIM1: Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Homooligomer and heterooligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA. Up-regulated by HMBA (hexamethylene bisacetamide). Down-regulated by estrogen. Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues. Belongs to the HEXIM family.

Protein type: Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; snRNA binding

Biological Process: heart development; negative regulation of cyclin-dependent protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; transcription, DNA-dependent
Show more

Research Articles on HEXIM1

1. PPM1G phosphatase directly binds 7SK RNA and the kinase inhibitor Hexim1 once P-TEFb has been released from the 7SK snRNP.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HEXIM1 antibody

 Reference Product  PubMed Publications
 CDK9 antibody  >55 publications with HEXIM1 and CDK9
 CCNT1 antibody  >24 publications with HEXIM1 and CCNT1
 BRD4 antibody  >21 publications with HEXIM1 and BRD4
 LARP7 antibody  >14 publications with HEXIM1 and LARP7
 MEPCE antibody  >8 publications with HEXIM1 and MEPCE
 CCNT2 antibody  >4 publications with HEXIM1 and CCNT2
 MDM2 antibody  >2 publications with HEXIM1 and MDM2

Diseases associated with anti-HEXIM1 antibody

 Disease Name  Pubmed Publications
 Hypertrophy Antibodies  >18 publications with HEXIM1 and Hypertrophy
 Heart Diseases Antibodies  >14 publications with HEXIM1 and Heart Diseases
 Disease Models, Animal Antibodies  >5 publications with HEXIM1 and Disease Models, Animal
 Inflammation Antibodies  >3 publications with HEXIM1 and Inflammation
 Abnormalities, Multiple Antibodies  >2 publications with HEXIM1 and Abnormalities, Multiple
 Lung Diseases Antibodies  >2 publications with HEXIM1 and Lung Diseases
 Fibrosis Antibodies  >2 publications with HEXIM1 and Fibrosis
 Necrosis Antibodies  >2 publications with HEXIM1 and Necrosis
 Abnormalities, Drug-Induced Antibodies  >1 publications with HEXIM1 and Abnormalities, Drug-Induced
 Hyperplasia Antibodies  >1 publications with HEXIM1 and Hyperplasia

Organs/Tissues associated with anti-HEXIM1 antibody

 Organ/Tissue Name  Pubmed Publications
 Mammary Gland Antibodies  >3 publications with HEXIM1 and Mammary Gland
 Pancreas Antibodies  >1 publications with HEXIM1 and Pancreas
 Thymus Antibodies  >1 publications with HEXIM1 and Thymus
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