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PSMA5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758822

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Product Overview

Online Datasheet in PDF
Product Name
PSMA5, Polyclonal Antibody
Full Product Name
Anti-PSMA5 Antibody Picoband
Product Gene Name
anti-PSMA5 antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Monkey, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
20,035 Da
OMIM
176844
3D Structure
ModBase 3D Structure for P28066
Immunogen
E coli-derived human PSMA5 recombinant protein (Position: M1-I241). Human PSMA5 shares 100% and 98.8% amino acid (aa) sequence identity with mouse and rat PSMA5, respectively.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.

Product Categories/Family for anti-PSMA5 antibody

Primary Antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-PSMA5 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-PSMA5 antibody
WB: 0.25-0.5ug/ml, Human, Monkey, Mouse, Rat
ICC/IF: 5ug/ml, Human
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-PSMA5 antibody

Figure 1. Western blot analysis of PSMA5 using anti-PSMA5 antibody (MBS1758822).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMA5 antigen affinity purified polyclonal antibody (#MBS1758822) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for PSMA5 at approximately 26 kDa. The expected band size for PSMA5 is at 26 kDa.
Rabbit Anti-PSMA5 Polyclonal Antibody – Cat# MBS1758822

Immunofluorescence (IF) of anti-PSMA5 antibody

Figure 2. IF analysis of PSMA5 using anti-PSMA5 antibody (MBS1758822).
PSMA5 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (#MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-PSMA5 Antibody (MBS1758822) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG (MBS177405) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Rabbit Anti-PSMA5 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1758822

Flow Cytometry (FC/FACS) of anti-PSMA5 antibody

Figure 3. Flow Cytometry analysis of Hela cells using anti-PSMA5 antibody (MBS1758822).
Overlay histogram showing Hela cells stained with MBS1758822 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMA5 Antibody (MBS1758822, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-PSMA5 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758822

Product References and Citations for anti-PSMA5 antibody

1. Coux, O., Tanaka, K., Goldberg, A. L. Structure and functions of the 20S and 26S proteasomes. Ann. Rev. Biochem. 65: 801-847, 1996. 2. DeMartino, G. N., Orth, K., McCullough, M. L., Lee, L. W., Munn, T. Z., Moomaw, C. R., Dawson, P. A., Slaughter, C. A. The primary structures of four subunits of the human, high molecular weight proteinase, macropain (proteasome), are distinct but homologous. Biochim. Biophys. Acta 1079: 29-38, 1991. 3. Mayau, V., Baron, B., Buttin, G., Debatisse, M. Twelve genes, including the unassigned proteasome zeta subunit gene, ordered within the human 1p13 region. Mammalian Genome 9: 331-333, 1998.

Other Notes

Small volumes of anti-PSMA5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PSMA5 purchase

MBS1758822 is an antibody targeting PSMA5. Also known as Anti-PSMA5 Antibody Picoband. Reactive in Human, Monkey, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PSMA5. It may not necessarily be applicable to this product.

NCBI GI #
315138981
NCBI GeneID
5686
NCBI Accession #
NP_001186701.1
UniProt Primary Accession #
P28066
UniProt Secondary Accession #
Q3T1C1; Q6IBF7; B2R8F6; B4E2V4
UniProt Related Accession #
P28066
GenBank Nucleotide #
NM_001199772.1
NCBI Official Full Name
proteasome subunit alpha type-5 isoform 2
NCBI Official Synonym Full Names
proteasome (prosome, macropain) subunit, alpha type, 5
NCBI Official Symbol
PSMA5
NCBI Official Synonym Symbols
PSC5; ZETA
UniProt Protein Name
Proteasome subunit alpha type-5
UniProt Synonym Protein Names
Macropain zeta chain; Multicatalytic endopeptidase complex zeta chain; Proteasome zeta chain
UniProt Gene Name
PSMA5
UniProt Entry Name
PSA5_HUMAN
NCBI Protein Information
proteasome subunit alpha type-5; macropain subunit zeta; macropain zeta chain; multicatalytic endopeptidase complex zeta chain; proteasome alpha 5 subunit; proteasome component 5; proteasome subunit zeta; proteasome zeta chain

NCBI Summary for PSMA5

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]
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UniProt Comments for PSMA5

PSMA5: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. PSMA5 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly. Up-regulated in colon cancer cell lines. Up-regulated in fetal Down syndrome (DS) brain. May be the target of the transcriptional activator NFE2L2. Expressed in fetal brain. Belongs to the peptidase T1A family.

Protein type: Protease; Proteasome complex; EC 3.4.25.1

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; cytoplasm; nucleus; cytosol

Molecular Function: threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
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Research Articles on PSMA5

1. Selective upregulation of the ubiquitin-proteasome proteolytic pathway proteins, proteasome zeta chain and isopeptidase T in fetal Down syndrome.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PSMA5 antibody

 Reference Product  PubMed Publications
 PSMA1 antibody  >6 publications with PSMA5 and PSMA1
 PSMA3 antibody  >6 publications with PSMA5 and PSMA3
 PSMA6 antibody  >3 publications with PSMA5 and PSMA6
 PSMB2 antibody  >3 publications with PSMA5 and PSMB2
 PSMB1 antibody  >2 publications with PSMA5 and PSMB1
 PSMA7 antibody  >2 publications with PSMA5 and PSMA7
 PSMA2 antibody  >2 publications with PSMA5 and PSMA2
 PSMB4 antibody  >1 publications with PSMA5 and PSMB4
 PSMA4 antibody  >1 publications with PSMA5 and PSMA4

Pathways associated with anti-PSMA5 antibody

 Products by Pathway  Pathway Diagram
 AMER1 Mutants Destabilize The Destruction Complex Pathway antibodies  AMER1 Mutants Destabilize The Destruction Complex Pathway Diagram
 APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway antibodies  APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway Diagram
 APC Truncation Mutants Have Impaired AXIN Binding Pathway antibodies  APC Truncation Mutants Have Impaired AXIN Binding Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Securin Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Securin Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway antibodies  APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway Diagram
 APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway antibodies  APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway Diagram
 AUF1 (hnRNP D0) Destabilizes MRNA Pathway antibodies  AUF1 (hnRNP D0) Destabilizes MRNA Pathway Diagram
 AXIN Missense Mutants Destabilize The Destruction Complex Pathway antibodies  AXIN Missense Mutants Destabilize The Destruction Complex Pathway Diagram

Diseases associated with anti-PSMA5 antibody

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >4 publications with PSMA5 and Immune System Diseases
 Necrosis Antibodies  >2 publications with PSMA5 and Necrosis
 Hypertension Antibodies  >1 publications with PSMA5 and Hypertension
 Lung Neoplasms Antibodies  >1 publications with PSMA5 and Lung Neoplasms
 Adenocarcinoma Antibodies  >1 publications with PSMA5 and Adenocarcinoma
 Carcinoma, Hepatocellular Antibodies  >1 publications with PSMA5 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-PSMA5 antibody

 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >2 publications with PSMA5 and Prostate
 Blood Antibodies  >2 publications with PSMA5 and Blood
 Lung Antibodies  >2 publications with PSMA5 and Lung
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