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Beta-Tubulin (TUBB3) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9610328

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha-and beta-tubulin, each of about 55, 000 kDa. Antibodies against beta Tubulin are useful as… More details

$235 / 0.05 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
beta-Tubulin (TUBB3), Monoclonal Antibody
Full Product Name
beta-Tubulin Mouse Monoclonal Antibody
Product Gene Name
anti-TUBB3 antibody
Product Synonym Names
TUBB3, CDCBM, Beta III Tubulin, Class III beta-tubulin, TUBB4, Tubulin, beta 3, Tubulin beta-III, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin, beta 3 class III, CFEOM3A
Clonality
Monoclonal
Isotype
IgG1
Host
Mouse
Species Reactivity
Human, Mouse, Rat, Sheep, Rabbit, Dog, Monkey, Hamster, Chicken
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl.
Purity/Purification
Peptide affinity purification
Concentration
1mg/ml (lot specific)
Specificity
beta-Tubulin Mouse Monoclonal antibody detects endogenous levels of total beta-Tubulin protein.
Molecular Weight
55 kD
3D Structure
ModBase 3D Structure for Q13509
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
Full-length beta-tubulin protein of human
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-TUBB3 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF)/Immunocytochemistry (ICC)
Application Notes for anti-TUBB3 antibody
WB: 1:1000-1:500000
IHC: 1:200
IF/ICC: 1:200

The optimal dilutions should be determined by the end user.

Western Blot (WB) of anti-TUBB3 antibody

Western blot analysis of hsc70 using various lysates.Lanes 1 - 2: Merged signal (red and green). Green - AF5187 observed at 70kDa. Red - loading control, MBS9610328, observed at 55 kDa. Blots were developed with Goat Anti-Rabbit IgG(H+L)FITC–conjugated (MBS9612520) and Goat Anti-Mouse IgG(H+L) AlexaFluor 594–conjugated (MBS9612517) secondary antibodies
Mouse Anti-TUBB3 Monoclonal Antibody – Cat# MBS9610328

Immunofluorescence (IF) of anti-TUBB3 antibody

IF/ICC staining of rat intestine tissue using beta-Tubulin MouseMonoclonal Antibody.
Mouse Anti-TUBB3 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS9610328

Immunohistochemistry (IHC) of anti-TUBB3 antibody

MBS9610328 at 1/100 staining Human colorectal cancer by IHC-P.The sample was formaldehyde fixed and a heat mediatedantigen retrieval step in citrate buffer was performed. Thesample was then blocked and incubated with the primaryantibody at 4°C overnight. An HRP conjugated anti-Mouseantibody was used as the secondary antibody.
Mouse Anti-TUBB3 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9610328

Product References and Citations for anti-TUBB3 antibody

Deng H, Xu H, Zhang X, Sun Y, Wang R, Brann D, Yang F; Journal: Toxicol Appl Pharmacol. Protective effect of Ac-SDKP on alveolar epithelial cells through inhibition of EMT via TGF-beta1/ROCK1 pathway in silicosis in rat. Yingying X, Caijuan W, Yenan Y, Yuqin T, Xueqin C, Zhongming W; Journal: Med Sci Monit.

Other Notes

Small volumes of anti-TUBB3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TUBB3 purchase

MBS9610328 is an antibody targeting TUBB3. Also known as beta-Tubulin Mouse Monoclonal Antibody. Reactive in Human, Mouse, Rat, Sheep, Rabbit, Dog, Monkey, Hamster, Chicken. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TUBB3. It may not necessarily be applicable to this product.

NCBI GI #
308235963
NCBI GeneID
10381
NCBI Accession #
NP_001184110.1
UniProt Primary Accession #
Q13509
UniProt Secondary Accession #
Q9BTZ0; Q9BW10; A8K854
UniProt Related Accession #
Q13509
GenBank Nucleotide #
NM_001197181.1
NCBI Official Full Name
tubulin beta-3 chain isoform 2
NCBI Official Synonym Full Names
tubulin beta 3 class III
NCBI Official Symbol
TUBB3
NCBI Official Synonym Symbols
CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A
UniProt Protein Name
Tubulin beta-3 chain
UniProt Synonym Protein Names
Tubulin beta-4 chain; Tubulin beta-III
Protein Family
Tubulin
UniProt Gene Name
TUBB3
UniProt Synonym Gene Names
TUBB4
NCBI Protein Information
tubulin beta-3 chain

NCBI Summary for TUBB3

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]
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UniProt Comments for TUBB3

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.

Research Articles on TUBB3

1. The remarkable high prevalence of TUBB3 expression in head and neck squamous cell carcinomas emphasizes its putative relevance as a target for future drugs targeting TUBB3

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TUBB3 antibody

 Products by Pathway  Pathway Diagram
 Chaperonin-mediated Protein Folding Pathway antibodies  Chaperonin-mediated Protein Folding Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway antibodies  Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway Diagram
 Diurnally Regulated Genes With Circadian Orthologs Pathway antibodies  Diurnally Regulated Genes With Circadian Orthologs Pathway Diagram
 Formation Of Tubulin Folding Intermediates By CCT/TriC Pathway antibodies  Formation Of Tubulin Folding Intermediates By CCT/TriC Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram

Diseases associated with anti-TUBB3 antibody

 Disease Name  Pubmed Publications
 Fibrosis Of Extraocular Muscles, Congenital, 3A, with or without Extraocular Involvement Antibodies  >514 publications with TUBB3 and Fibrosis Of Extraocular Muscles, Congenital, 3A, with or without Extraocular Involvement
 Neoplasms Antibodies  >47 publications with TUBB3 and Neoplasms
 Nervous System Diseases Antibodies  >16 publications with TUBB3 and Nervous System Diseases
 Ovarian Neoplasms Antibodies  >12 publications with TUBB3 and Ovarian Neoplasms
 Breast Neoplasms Antibodies  >9 publications with TUBB3 and Breast Neoplasms
 Fibrosis Antibodies  >8 publications with TUBB3 and Fibrosis
 Neoplasms, Experimental Antibodies  >5 publications with TUBB3 and Neoplasms, Experimental
 Brain Diseases Antibodies  >4 publications with TUBB3 and Brain Diseases
 Carcinoma, Squamous Cell Antibodies  >4 publications with TUBB3 and Carcinoma, Squamous Cell
 Eye Diseases Antibodies  >4 publications with TUBB3 and Eye Diseases

Organs/Tissues associated with anti-TUBB3 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >22 publications with TUBB3 and Lung
 Brain Antibodies  >20 publications with TUBB3 and Brain
 Nerve Antibodies  >10 publications with TUBB3 and Nerve
 Muscle Antibodies  >9 publications with TUBB3 and Muscle
 Eye Antibodies  >9 publications with TUBB3 and Eye
 Heart Antibodies  >8 publications with TUBB3 and Heart
 Embryonic Tissue Antibodies  >6 publications with TUBB3 and Embryonic Tissue
 Testis Antibodies  >4 publications with TUBB3 and Testis
 Lymph Antibodies  >3 publications with TUBB3 and Lymph
 Lymph Node Antibodies  >3 publications with TUBB3 and Lymph Node
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