• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

AP2M1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6274086

This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton… More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
AP2M1, Polyclonal Antibody
Full Product Name
AP2M1, CT (AP2M1, CLAPM1, KIAA0109, AP-2 complex subunit mu, AP-2 mu chain, Adapter-related protein complex 2 mu subunit, Adaptin-mu2, Adaptor protein complex AP-2 subunit mu, Clathrin assembly protein complex 2 medium chain, Clathrin coat assembly protei
Product Gene Name
anti-AP2M1 antibody
Product Synonym Names
AP2M1; CLAPM1; KIAA0109; AP-2 complex subunit mu; AP-2 mu chain; Adapter-related protein complex 2 mu subunit; Adaptin-mu2; Adaptor protein complex AP-2 subunit mu; Clathrin assembly protein complex 2 medium chain; Clathrin coat assembly protein AP50; Clathrin coat-associated protein AP50; HA2 50kD subunit; Plasma membrane adaptor AP-2 50kD protein
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
435
Chromosome Location
Chromosome: 3; NC_000003.11 (183892634..183901879). Location: 3q28
OMIM
601024
3D Structure
ModBase 3D Structure for Q96CW1
Immunogen
AP2M1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 386-415 amino acids from the C-terminal region of human AP2M1.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-AP2M1 antibody

Antibodies; Clathrin
Applications Tested/Suitable for anti-AP2M1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-AP2M1 antibody
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-AP2M1 antibody

AP2M1 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the AP2M1 antibody detected the AP2M1 protein (arrow).
Rabbit Anti-AP2M1 Polyclonal Antibody – Cat# MBS6274086

Flow Cytometry (FC/FACS) of anti-AP2M1 antibody

AP2M1 Antibody (C-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-AP2M1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6274086

Product References and Citations for anti-AP2M1 antibody

Kahlfeldt, N., et al. J. Biol. Chem. 285(4):2734-2749(2010). Boucrot, E., et al. PLoS ONE 5 (5), E10597 (2010): Yoshida, T., et al. Int. J. Mol. Med. 24(2):233-246(2009). Levecque, C., et al. Am. J. Physiol., Cell Physiol. 297 (1), C160-C168 (2009): Stanasila, L., et al. Mol. Pharmacol. 74(3):562-573(2008).

Other Notes

Small volumes of anti-AP2M1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AP2M1 purchase

MBS6274086 is an antibody targeting AP2M1. Also known as AP2M1, CT (AP2M1, CLAPM1, KIAA0109, AP-2 complex subunit mu, AP-2 mu chain, Adapter-related protein complex 2 mu subunit, Adaptin-mu2, Adaptor protein complex AP-2 subunit mu, Clathrin assembly protein complex 2 medium chain, Clathrin coat assembly protei. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for AP2M1. It may not necessarily be applicable to this product.

NCBI GI #
68799814
NCBI GeneID
1173
NCBI Accession #
NP_001020376.1
NCBI Related Accession #
NP_004059.2
UniProt Primary Accession #
Q96CW1
UniProt Secondary Accession #
P20172; P53679; A6NE12; D3DNT1
UniProt Related Accession #
Q96CW1
GenBank Nucleotide #
NM_001025205.1
NCBI Official Full Name
AP-2 complex subunit mu isoform b
NCBI Official Synonym Full Names
adaptor-related protein complex 2, mu 1 subunit
NCBI Official Symbol
AP2M1
NCBI Official Synonym Symbols
mu2; AP50; CLAPM1
UniProt Protein Name
AP-2 complex subunit mu
UniProt Synonym Protein Names
AP-2 mu chain; Adaptin-mu2; Adaptor protein complex AP-2 subunit mu; Adaptor-related protein complex 2 subunit mu; Clathrin assembly protein complex 2 mu medium chain; Clathrin coat assembly protein AP50; Clathrin coat-associated protein AP50; HA2 50 kDa subunit; Plasma membrane adaptor AP-2 50 kDa protein
UniProt Gene Name
AP2M1
UniProt Synonym Gene Names
CLAPM1; KIAA0109
UniProt Entry Name
AP2M1_HUMAN
NCBI Protein Information
AP-2 complex subunit mu; adaptin-mu2; AP-2 mu chain; AP-2 mu 2 chain; HA2 50 kDA subunit; clathrin coat adaptor protein AP50; clathrin coat assembly protein AP50; clathrin coat-associated protein AP50; adaptor protein complex AP-2 subunit mu; clathrin ada

UniProt Comments for AP2M1

AP2M1: a protein of the adaptor complexes medium subunit family. A subunit of the heterotetrameric coat assembly protein complex 2 (AP2) which links clathrin to receptors in coated vesicles. Interacts with the cytoplasmic tails of membrane proteins and polyphosphoinositide-containing lipids. Is not required for clathrin-coated vesicle formation at the plasma membrane, but that it is one of several endocytic adaptors required for the uptake of certain cargo proteins. Is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP-2 is a heterotetramer composed of two large chains (alpha and beta), a medium chain (AP50) and a small chain (AP17).

Protein type: Vesicle; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: mitochondrion; lysosomal membrane; plasma membrane; AP-2 adaptor complex; cytosol

Molecular Function: protein binding; low-density lipoprotein receptor binding; transporter activity; signal sequence binding; lipid binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; intracellular protein transport; axon guidance; synaptic transmission; viral reproduction; nerve growth factor receptor signaling pathway; ephrin receptor signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class II; regulation of defense response to virus by virus
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-AP2M1 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Arf1 Pathway antibodies  Arf1 Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 EGFR Downregulation Pathway antibodies  EGFR Downregulation Pathway Diagram
 Endocrine And Other Factor-regulated Calcium Reabsorption Pathway antibodies  Endocrine And Other Factor-regulated Calcium Reabsorption Pathway Diagram
 Endocrine And Other Factor-regulated Calcium Reabsorption Pathway antibodies  Endocrine And Other Factor-regulated Calcium Reabsorption Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram

Diseases associated with anti-AP2M1 antibody

 Disease Name  Pubmed Publications
 Hepatitis Antibodies  >1 publications with AP2M1 and Hepatitis
 Liver Diseases Antibodies  >1 publications with AP2M1 and Liver Diseases
 Fibrosis Antibodies  >1 publications with AP2M1 and Fibrosis
 Carcinoma, Hepatocellular Antibodies  >1 publications with AP2M1 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-AP2M1 antibody

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >1 publications with AP2M1 and Heart
 Liver Antibodies  >1 publications with AP2M1 and Liver
 Brain Antibodies  >1 publications with AP2M1 and Brain
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000