Rabbit Anti-ASAH2 Polyclonal Antibody – Cat# MBS6275475

Western Blot analysis in mouse bladder tissue lysates (35ug/lane) usingMBS6005122. This demonstrates that MBS6005122 detected the ASAH2 protein (arrow).

ASAH2 Antibody – Rabbit Polyclonal

Name: ASAH2 Antibody; Polyclonal; Rabbit; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6275475
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6275475

Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its… More details

$1060 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ASAH2, Polyclonal Antibody

Full Product Name

ASAH2, CT (ASAH2, HNAC1, Neutral ceramidase, Acylsphingosine deacylase 2, BCDase, LCDase, N-acylsphingosine amidohydrolase 2, Non-lysosomal ceramidase, Neutral ceramidase soluble form) (MaxLight 550)

Product Gene Name

anti-ASAH2 antibody

Product Synonym Names

ASAH2; HNAC1; Neutral ceramidase; Acylsphingosine deacylase 2; BCDase; LCDase; N-acylsphingosine amidohydrolase 2; Non-lysosomal ceramidase; Neutral ceramidase soluble form

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

Purity/Purification

Purified by Protein A Affinity Chromatography.

Specificity

Recognizes human ASAH2. Species Crossreactivity: mouse

Preparation and Storage

Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Molecular Weight

18,026 Da

OMIM

611202

3D Structure

ModBase 3D Structure for Q9NR71

Immunogen

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 734-762 in the C-terminal region of human ASAH2, UniProt Accession #NP_001072984.1; Q9NR71; P0C7U2; P0C7U1.

Conjugate

MaxLight550

Note

Preservative Free

Product Categories/Family for anti-ASAH2 antibody

Antibodies; Enzymes

Applications Tested/Suitable for anti-ASAH2 antibody

Western Blot (WB), FLISA

Application Notes for anti-ASAH2 antibody

WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Product References and Citations for anti-ASAH2 antibody

1. Uchida, Y., et al. J. Invest. Dermatol. 130(10):2472-2480(2010). 2. Hong, K.K., et al. J. Korean Med. Sci. 22(5):862-867(2007). 3. Ohlsson, L., et al. Biochimie 89(8):950-960(2007). 4. Avramopoulos, D., et al. Neurogenetics 8(2):111-120(2007). 5. Galadari, S., et al. Biochem. J. 393 (PT 3), 687-695 (2006).

Other Notes

Small volumes of anti-ASAH2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ASAH2 purchase

MBS6275475 is an antibody targeting ASAH2. Also known as ASAH2, CT (ASAH2, HNAC1, Neutral ceramidase, Acylsphingosine deacylase 2, BCDase, LCDase, N-acylsphingosine amidohydrolase 2, Non-lysosomal ceramidase, Neutral ceramidase soluble form) (MaxLight 550). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ASAH2. It may not necessarily be applicable to this product.

NCBI GI #

118442845

NCBI GeneID

653308

NCBI Accession #

NP_001072984.1

UniProt Primary Accession #

Q9NR71

UniProt Secondary Accession #

P0C7U2; P0C7U1

UniProt Related Accession #

P0C7U1

GenBank Nucleotide #

NM_001079516.3

NCBI Official Full Name

putative inactive neutral ceramidase B isoform 1

NCBI Official Synonym Full Names

N-acylsphingosine amidohydrolase 2B

NCBI Official Symbol

ASAH2B

NCBI Official Synonym Symbols

ASAH2C; ASAH2L; bA98I6.3; bA449O16.3

UniProt Protein Name

Neutral ceramidase

UniProt Synonym Protein Names

Acylsphingosine deacylase 2; BCDase; LCDase; hCD; N-acylsphingosine amidohydrolase 2; Non-lysosomal ceramidaseNeutral ceramidase soluble form

Protein Family

Neutral ceramidase

UniProt Gene Name

ASAH2

UniProt Synonym Gene Names

HNAC1; N-CDase; NCDase; hCD

UniProt Entry Name

ASAH2_HUMAN

NCBI Protein Information

putative inactive neutral ceramidase B

UniProt Comments for ASAH2

ASAH2: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. Belongs to the neutral ceramidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Hydrolase; EC 3.5.1.23; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 10q11.21

Cellular Component: mitochondrion; plasma membrane; integral to membrane

Molecular Function: ceramidase activity

Biological Process: sphingolipid metabolic process; apoptosis; glycosphingolipid metabolic process; ceramide metabolic process; signal transduction

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ASAH2 antibody

Products by Pathway	Pathway Diagram
Ceramide Degradation Pathway antibodies	Ceramide Degradation Pathway Diagram
Sphingosine And Sphingosine-1-phosphate Metabolism Pathway antibodies	Sphingosine And Sphingosine-1-phosphate Metabolism Pathway Diagram