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ATG10 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9413459

Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ATG10, Polyclonal Antibody
Full Product Name
ATG10 Antibody
Product Gene Name
Product Synonym Names
APG10; APG10L; pp12616
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Purity/Purification
Antigen affinity purification.
Concentration
2.4 mg/ml (lot specific)
Specificity
The antibody detects endogenous levels of total ATG10 protein.
Preparation and Storage
Store at -20 degree C
Molecular Weight
14,217 Da
OMIM
610800
3D Structure
ModBase 3D Structure for Q9H0Y0
Immunogen Type
Recombinant Protein
Immunogen Description
Fusion protein corresponding to a region derived from internal residues of human autophagy related 10
Target Name
ATG10

Product Categories/Family for anti-ATG10 antibody

Applications Tested/Suitable for anti-ATG10 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ATG10 antibody
Western blotting: 1:200-1:1000
Immunohistochemistry: 1:50-1:200

Testing Data of anti-ATG10 antibody

Gel: 10%SDS-PAGE Lysates (from left to right): Mouse liver tissue Amount of lysate: 50ug per lane Primary antibody: 1/500 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute
Rabbit Anti-ATG10 Polyclonal Antibody – Cat# MBS9413459

Immunohistochemistry (IHC) of anti-ATG10 antibody

Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/60.
Rabbit Anti-ATG10 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9413459

Other Notes

Small volumes of anti-ATG10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATG10 purchase

MBS9413459 is an antibody targeting ATG10. Also known as ATG10 Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG10. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ubiquitin-like-conjugating enzyme ATG10
NCBI Official Synonym Full Names
autophagy related 10
NCBI Official Symbol
ATG10
NCBI Official Synonym Symbols
APG10; APG10L; pp12616
UniProt Protein Name
Ubiquitin-like-conjugating enzyme ATG10
UniProt Synonym Protein Names
Autophagy-related protein 10; APG10-like
UniProt Gene Name
ATG10
UniProt Synonym Gene Names
APG10L; APG10-like
UniProt Entry Name
ATG10_HUMAN
NCBI Protein Information
ubiquitin-like-conjugating enzyme ATG10

NCBI Summary for ATG10

Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM, Mar 2008]
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UniProt Comments for ATG10

ATG10: E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Belongs to the ATG10 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Ubiquitin conjugating system; Autophagy

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: cytoplasm; intracellular

Molecular Function: APG12 conjugating enzyme activity; ligase activity

Biological Process: protein transport; protein modification by small protein conjugation; protein amino acid lipidation; positive regulation of protein modification process; autophagy
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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