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ATG12 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6463477

MBS6463477 is an antibody targeting ATG12. Also known as ATG12, NT (APG12, APG12L, HAPG12, APG12 Autophagy 12-like, APG12-like, Apg12 (Autophagy, yeast) Homolog, Autophagy-related Protein 12) (PE). Reactive in Human, Mouse. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ATG12, Polyclonal Antibody
Full Product Name
ATG12, NT (APG12, APG12L, HAPG12, APG12 Autophagy 12-like, APG12-like, Apg12 (Autophagy, yeast) Homolog, Autophagy-related Protein 12) (PE)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein G Affinity Chromatography.
Specificity
Recognizes human APG12L. Species Crossreactivity: mouse.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
21kDa
OMIM
609608
3D Structure
ModBase 3D Structure for O94817
Immunogen
Synthetic peptide selected from the N-terminal region of human APG12L (KLH).
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-ATG12 antibody

Applications Tested/Suitable for anti-ATG12 antibody
Immunofluorescence (IF), FLISA, Western Blot (WB)
Application Notes for anti-ATG12 antibody
IF: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-ATG12 antibody

Western blot analysis of hAPG12L-T23 in MCF-7 cell line lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.
Rabbit Anti-ATG12 Polyclonal Antibody – Cat# MBS6463477

Western Blot (WB) of anti-ATG12 antibody

Western blot analysis of hAPG12L-T23 in mouse brain tissue lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.
Rabbit Anti-ATG12 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6463477

Testing Data of anti-ATG12 antibody

Mouse leukaemic monocyte macrophage cells treated with vinblastine for 1 hr. APG12L antibody detected punctuate staining indicative of nascent autophagic vacuole structures. Data courtesy of Dr. Barry Boland, Department of Pharmacology, Oxford University.
Rabbit Anti-ATG12 Polyclonal Antibody, Testing Data – Cat# MBS6463477

Product References and Citations for anti-ATG12 antibody

1. Tanida, I., et al., Biochem. Biophys. Res. Commun. 296(5):1164-1170 (2002). 2. Tanida, I., et al., J. Biol. Chem. 277(16):13739-13744 (2002). 3. Mizushima, N., et al., J. Biol. Chem. 273(51):33889-33892 (1998). 4. Ueno, K., et al., Hum. Genet. 102(1):63-68 (1998).

Other Notes

Small volumes of anti-ATG12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG12. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ubiquitin-like protein ATG12 isoform 1
NCBI Official Synonym Full Names
autophagy related 12
NCBI Official Symbol
ATG12
NCBI Official Synonym Symbols
APG12; FBR93; APG12L; HAPG12
UniProt Protein Name
Ubiquitin-like protein ATG12
UniProt Synonym Protein Names
Autophagy-related protein 12; APG12-like
Protein Family
UniProt Gene Name
ATG12
UniProt Synonym Gene Names
APG12; APG12L; APG12-like
UniProt Entry Name
ATG12_HUMAN
NCBI Protein Information
ubiquitin-like protein ATG12

NCBI Summary for ATG12

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]
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UniProt Comments for ATG12

ATG12: Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5. Ubiquitous. Belongs to the ATG12 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 5q21-q22

Cellular Component: pre-autophagosomal structure membrane

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: mitochondrion degradation; innate immune response; C-terminal protein lipidation; negative regulation of interferon type I production; cellular response to nitrogen starvation; autophagic vacuole formation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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