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ATG5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6276200

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves… More details

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Product Overview

Online Datasheet in PDF
Product Name
ATG5, Polyclonal Antibody
Full Product Name
ATG5, NT (ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein) (MaxLight 490)
Product Gene Name
anti-ATG5 antibody
Product Synonym Names
ATG5; APG5L; ASP; Autophagy protein 5; APG5-like; Apoptosis-specific protein
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
275
OMIM
604261
3D Structure
ModBase 3D Structure for Q9H1Y0
Immunogen
ATG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-term of human APG5L.
Conjugate
MaxLight490
Note
Preservative Free

Product Categories/Family for anti-ATG5 antibody

Antibodies; Autophagy
Applications Tested/Suitable for anti-ATG5 antibody
Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-ATG5 antibody
IF: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.

Immunofluorescence (IF) of anti-ATG5 antibody

Fluorescent image of U251 cells stained with ATG5 (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1812a ATG5 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Rabbit Anti-ATG5 Polyclonal Antibody – Cat# MBS6276200

Western Blot (WB) of anti-ATG5 antibody

APG5L Antibody (D3) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the APG5L antibody detected the APG5L protein (arrow).
Rabbit Anti-ATG5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6276200

Western Blot (WB) of anti-ATG5 antibody

The anti-APG5L Pab is used in Western blot to detect APG5L in mouse brain tissue lysate.
Rabbit Anti-ATG5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6276200

Western Blot (WB) of anti-ATG5 antibody

Western blot analysis of APG5L (arrow) using rabbit polyclonal APG5L Antibody (D3). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5L gene.
Rabbit Anti-ATG5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6276200

Immunohistochemistry (IHC) of anti-ATG5 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-ATG5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6276200

Product References and Citations for anti-ATG5 antibody

Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005). Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005). Greenberg JT. Dev Cell. 8(6):799-801. (2005). Levine B. Cell. 120(2):159-62. (2005). Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004). Hammond E.M., et al. FEBS Lett. 425:391-395(1998). Strausberg R.L., et al. PNAS 99:16899-16903(2002). Grand R.J.A., et al. Exp. Cell Res. 218:439-451(1995) Mizushima N., et al. J. Biol. Chem. 273:33889-33892(1998). Mizushima N., et al. J. Cell Biol. 152:657-668(2001).

Other Notes

Small volumes of anti-ATG5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATG5 purchase

MBS6276200 is an antibody targeting ATG5. Also known as ATG5, NT (ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein) (MaxLight 490). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG5. It may not necessarily be applicable to this product.

NCBI GI #
17366828
NCBI GeneID
9474
NCBI Accession #
Q9H1Y0.2
UniProt Primary Accession #
Q9H1Y0
UniProt Secondary Accession #
O60875; Q5JVR2; Q68DI4; Q9H2B8; Q9HCZ7
UniProt Related Accession #
Q9H1Y0
NCBI Official Full Name
Autophagy protein 5
NCBI Official Synonym Full Names
autophagy related 5
NCBI Official Symbol
ATG5
NCBI Official Synonym Symbols
ASP; APG5; APG5L; hAPG5; APG5-LIKE
UniProt Protein Name
Autophagy protein 5
UniProt Synonym Protein Names
APG5-like; Apoptosis-specific protein
Protein Family
Autophagy protein
UniProt Gene Name
ATG5
UniProt Synonym Gene Names
APG5L; ASP
UniProt Entry Name
ATG5_HUMAN
NCBI Protein Information
autophagy protein 5; apoptosis-specific protein; ATG5 autophagy related 5 homolog

UniProt Comments for ATG5

ATG5: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5- ATG12 conjugate. By apoptotic stimuli. Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Belongs to the ATG5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Autophagy; Apoptosis

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: membrane; pre-autophagosomal structure membrane; cytoplasm; autophagic vacuole; axoneme

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: response to drug; response to fungus; apoptosis; ventricular cardiac muscle cell development; C-terminal protein lipidation; post-translational protein modification; vasodilation; regulation of release of sequestered calcium ion into cytosol; cellular response to nitrogen starvation; heart contraction; regulation of cytokine secretion during immune response; mitochondrion degradation; autophagy; innate immune response; negative regulation of protein ubiquitination; blood vessel remodeling; negative regulation of interferon type I production; otolith development; autophagic vacuole formation; negative regulation of apoptosis
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Research Articles on ATG5

1. Globular adiponectin restores ethanol-suppressed autophagy-related protein 5 (Atg5) expression in HepG2 cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ATG5 antibody

 Reference Product  PubMed Publications
 ATG12 antibody  >223 publications with ATG5 and ATG12
 BECN1 antibody  >194 publications with ATG5 and BECN1
 ATG3 antibody  >53 publications with ATG5 and ATG3
 ATG16L1 antibody  >48 publications with ATG5 and ATG16L1
 CASP8 antibody  >27 publications with ATG5 and CASP8
 FADD antibody  >21 publications with ATG5 and FADD
 SIRT1 antibody  >8 publications with ATG5 and SIRT1
 DDX58 antibody  >5 publications with ATG5 and DDX58
 MAVS antibody  >4 publications with ATG5 and MAVS
 WDFY3 antibody  >1 publications with ATG5 and WDFY3

Pathways associated with anti-ATG5 antibody

 Products by Pathway  Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Negative Regulators Of RIG-I/MDA5 Signaling Pathway antibodies  Negative Regulators Of RIG-I/MDA5 Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 RIG-I-like Receptor Signaling Pathway antibodies  RIG-I-like Receptor Signaling Pathway Diagram
 RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways antibodies  RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
 Regulation Of Autophagy Pathway antibodies  Regulation Of Autophagy Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram
 Shigellosis Pathway antibodies  Shigellosis Pathway Diagram

Diseases associated with anti-ATG5 antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >57 publications with ATG5 and Necrosis
 Cardiovascular Diseases Antibodies  >55 publications with ATG5 and Cardiovascular Diseases
 Inflammation Antibodies  >55 publications with ATG5 and Inflammation
 Disease Models, Animal Antibodies  >47 publications with ATG5 and Disease Models, Animal
 Liver Diseases Antibodies  >42 publications with ATG5 and Liver Diseases
 Gastrointestinal Diseases Antibodies  >35 publications with ATG5 and Gastrointestinal Diseases
 Heart Diseases Antibodies  >34 publications with ATG5 and Heart Diseases
 Carcinoma, Hepatocellular Antibodies  >27 publications with ATG5 and Carcinoma, Hepatocellular
 Breast Neoplasms Antibodies  >25 publications with ATG5 and Breast Neoplasms
 Liver Neoplasms Antibodies  >24 publications with ATG5 and Liver Neoplasms

Organs/Tissues associated with anti-ATG5 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >91 publications with ATG5 and Blood
 Brain Antibodies  >88 publications with ATG5 and Brain
 Liver Antibodies  >69 publications with ATG5 and Liver
 Muscle Antibodies  >59 publications with ATG5 and Muscle
 Lung Antibodies  >58 publications with ATG5 and Lung
 Heart Antibodies  >52 publications with ATG5 and Heart
 Kidney Antibodies  >35 publications with ATG5 and Kidney
 Eye Antibodies  >31 publications with ATG5 and Eye
 Bone Antibodies  >31 publications with ATG5 and Bone
 Prostate Antibodies  >25 publications with ATG5 and Prostate
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